Browse Journals
Volume 62, Number 1, February 2025
Peer Reviewed Manuscripts
Utilizing Boron to Improve Sugarbeet Yield, Quality, and Cercospra Leaf Spot Management
Lacie Thomas1, Jaime Willbur1, and Kurt Steinke1
1 Dept. of Plant, Soil, and Microbial Sciences, Plant and Soil Sciences Building, 1066 Bogue Street, Michigan State University, East Lansing, MI 48824
Corresponding author: Kurt Steinke ([email protected])
Abstract View Article
Cercospora beticola, the causal pathogen of Cercospora leaf spot (CLS), reduces sugarbeet yield up to 40% when not managed appropriately. Plant defoliation caused by the disease impacts root size, sugar quality, leaf regrowth, and impurity concentrations. Management strategies including boron-containing compounds have shown fungistatic properties potentially reducing field disease severity. Field studies were established to investigate the effects of foliar applied boron (B) on sugarbeet growth and CLS severity. Treatments included a standard fungicide program, three foliar B treatments (0.11, 0.28, or 0.56 kg sodium tetraborate ha-1 (0.1, 0.25, or 0.5 lb A-1)) applied at 10 to 14 day intervals individually, the same three foliar B treatments applied in conjunction with a standard fungicide program, and a non-treated check for comparison. Foliar B did not reduce CLS in field environments across site years. In vitro analysis of C. beticola response to B demonstrated lower EC50 values with sodium tetraborate than boric acid. However, both sodium tetraborate and boric acid increased EC50 values compared to the thiophanate-methyl control. Reduced control options combined with increased CLS resistance will require further evaluation of alternative control measures possibly including various B timings and increased B concentrations.
Introgression of the Cercospora Leaf Spot (CLS) Disease Resistance Trait From KEMS06 Sugar Beet Germplasm Into Two Double-Haploid Breeding Lines, KDH4-9 and KDH13
Eric D. Vincill1*, Imad A. Eujayl1, Rajtilak Majumdar1, and Carl A. Strausbaugh1
1United States Department of Agriculture-Agricultural Research Service
Northwest Irrigation and Soils Research Laboratory (USDA-ARS NWISRL)
Kimberly, ID 83341 U.S.A.
Abstract View Article
Cercospora leaf spot (CLS), caused by the fungal pathogen Cercospora beticola, is considered one of the most destructive foliar diseases of sugar beet (Beta vulgaris). CLS causes leaf damage often followed by intensive foliage regrowth which can result in considerable sugar losses unless timely applications of fungicides are applied throughout the growing season. While progress is being made towards improving CLS resistance in commercially available varieties of sugar beet, disease-prone areas still require multiple applications of fungicides during the growing season to keep sugar beet crop losses to a minimum. Recently, the sugar beet germplasm KEMS06 (PI 683514) derived from an ethyl methanesulfonate (EMS)-mutagenized population was found to have high CLS resistance. Sibling cohorts of KEMS06 have shown diverse and tractable phenotypes suggesting that the CLS resistance trait from KEMS06 may contain genetic underpinnings that have not been leveraged before in commercially available sugar beet varieties. In this report, we describe the use of greenhouse CLS assays to characterize the CLS resistance trait from the KEMS06 sugar beet germplasm. Two different hybrid (resistant x susceptible) crosses were generated, and the disease resistance segregation patterns with corresponding variance scores in the successive filial generations were measured. The results presented in this report strongly suggest the CLS resistance trait in KEMS06 is directly linked to underlying genetic determinants that are segregating in-step across the population in a quantitative manner. Heritability of the KEMS06 CLS resistance trait was tractable into the F2, F3, and F4 filial generations. By following a single plant descent methodology—expedited by the use of self-fertility traits in the parental lines—complete introgression of the KEMS06 CLS resistance trait was observed in progeny families. In addition, the greenhouse methods described within this report accelerated our CLS resistance trait discovery and development pre-breeding timeline by reducing the time that would normally be required to introgress and fix disease resistance traits into new sugar beet germplasm by one to
Microbial Isolates From North American Sugar Beet Factory Juices and Biofilms
Yunci Qi 1, Evan Terrell2, Gillian O. Bruni2, Zianab Yassin3, and Shyam L. Kandel4
1 National Center for Agricultural Utilization Research, Peoria, IL 61604 USA; 2 Southern Regional Research Center, New Orleans, LA 70124 USA; 3 Beet Sugar Development Foundation, Denver, CO 80203, and 4Edward T. Schafer Agricultural Research Center, Fargo, ND 58102-2765;
Abstract View Article
During sugar beet processing, microbes from infected roots, storage piles, and soils carry over throughout sugar extraction, creating operational challenges and resulting in sucrose losses. In this study, diffusion tower juice and biofilm samples were obtained from 18 North American sugar beet factories for microbial sampling to characterize issues relating to raw sugar manufacturing, such as increased sample viscosity from bacterial exopolysaccharides (EPS) production. A broad sampling strategy was applied to obtain diverse microbial isolates for experimental characterization. In total, 379 isolates, belonging to 22 genera, were obtained from 33 diffuser tower juice samples, and 233 isolates, belonging to 26 genera, were obtained from 21 biofilm samples. A subset of 54 strains representing the most common genera (Leuconostoc, Peribacillus, Bacillus, Pantoea, Rahnella, Acinetobacter, and Weissella) were grown in high sucrose-containing medium. Among these, 10 strains were identified as capable of greatly increasing viscosity in the flask cultures, likely due to EPS production.
Abstracts from the latest meeting
Agronomy - Oral
Adjusting fertilizer strategies to address new challenges in sugarbeet production including early harvest, varietal differences, and sugar quality.
STEINKE, KURT*, STORM SOAT, and ANDREW CHOMAS, Michigan State University, Plant and Soil Sciences Building, Department of Plant, Soil, and Microbial Sciences, 1066 Bogue Street, East Lansing, MI 48824.
Abstract View Article
Climate variability including extended wet and dry periods during the growing season or increased freeze-thaw cycling during processing operations can adversely impact sugar quality. To complete sugar processing prior to warmer spring temperatures, the Michigan sugarbeet harvest season now extends from early harvest (i.e., mid-late August) through conventional harvest (beginning mid-late October). A recent initiative that emphasizes improved beet quality as compared to yield has practitioners questioning which fertilizer management strategies to target across a more variable climate, early harvest intervals, updated varietal characteristics, and when managing an N-responsive cropping system. Although management has mostly evolved to being site- or field-specific, little work has been done investigating how nutrient management may change based on sugarbeet varietal characteristics. More defensive varieties with greater disease tolerance may respond differently compared to more aggressive varieties with greater tonnage. Multi-year field studies were established to evaluate sugarbeet varietal response (defensive vs. aggressive) to specific fertilizer management strategies in combination with early and conventional harvest intervals. The study was blocked by two harvest timings (early and conventional) and two varieties. All treatments (other than the check) received 67 kg N ha-1 applied 5 cm x 5 cm at planting. Five fertilizer strategies consisted of 1) non-treated control (NTC), 2) 67 kg N ha-1 applied 5×5 at planting (5×5 N only), 3) 67 kg N ha-1 applied 5×5 followed by 112 kg N ha-1 subsurface banded at 2-4 leaf growth stage for total N rate of 179 kg N ha-1 (5×5 + Sidedress N), 4) ) 67 kg N ha-1 applied 5×5 followed by 112 kg K2O ha-1 surface banded at 20-leaf growth stage (5×5 + liquid K), and 5) 67 kg N ha-1 applied 5×5 followed by 112 kg N ha-1 subsurface banded at 2-4 leaf growth stage plus 112 kg K2O ha-1 surface banded at 20-leaf growth stage for a total of 179 kg N ha-1 and 112 kg K2O ha-1 (All). Across early and conventional harvest timings, 179 kg N ha-1 produced optimal root yields and recoverable sucrose per hectare but peak recoverable sucrose per Mg was produced by starter N only in both years of early harvest timing and one of two years for conventional harvest timing. Liquid K2O application to either starter N only or split-applied N did not increase root yield or quality from similar treatments receiving only N fertilizer suggesting K management may be part of a broader soil management strategy for areas with below critical K concentrations rather than specifically targeted to enhance sugar production.
Simulated hail timing and severity on sugarbeet root yield and sucrose content.
PETERS, THOMAS J.*1, ALEXA L. LYSTAD2 and MARK E. ZARNSTORFF3, 1North Dakota State University and the University of Minnesota, Fargo 58102, 2KayJay Ag Services, Inc., Horace, ND 58047,3National Crop Insurance Services, Overland Park, KS 66210.
Abstract View Article
An unintended outcome of crop production is losses due to abiotic factors including wind, water, and temperature. Defoliation of sugarbeet from hail can occur in any month throughout the growing season and can cause sugarbeet damage ranging from virtually undetectable damage to complete defoliation of vegetative plant material. Sugarbeet will almost always recover and continue their growth cycle unless hail causes stand loss which occurs mostly when sugarbeet are seedlings. Modern-day sugarbeet are diploid varieties as compared to tetraploid and triploid varieties characterized by heavy leaf texture and a large bush style plant architecture produced in previous decades. Experiments were conducted at multiple locations in 2021, 2022, and 2023 to measure vegetative index, root yield, and percent sucrose following simulated hail treatments at different growth stages and intensity with diploid varieties. Experiments were initiated in April and May when conditions were appropriate for cultivation by seeding at approximately 161,700 seed per hectare. Treatments were six sugarbeet rows spaced 56 cm apart and 9.1 m in length. Stands were thinned to approximately 180 sugarbeet per 30.5 m row in rows two through five when sugarbeet were at the 2-lf stage. Treatments were 0%, 25%, 50%, 75% and 100% (only petioles remaining) loss of canopy to simulate hail implemented by removing leaves from plants with hand shears in mid-July, early August and end of August. Health and density of the sugarbeet canopy was quantified approximately 21, 42, and 63 days after treatment (DAT) by collecting normalized difference vegetation index (NDVI) data with a RapidScan CS-45 active sensor (Holland Scientific, Lincoln, NE). Sugarbeet was defoliated and harvested mechanically in mid to late September from the center two rows of each plot and weighed. A 10-kg sample was collected from each plot and analyzed for sucrose content and sugar loss to molasses by American Crystal Sugar Company (East Grand Forks, ND). Sugarbeet canopy 21 days after the 25% and 50% canopy loss simulated hail treatment was greater than 90% of the 0% treatment at Crookston in 2022. All treatments were greater than 90% of the 0% treatment, 42 DAT. Further, canopy regrowth from simulated hail in late-August occurred at the same rate as canopy regrowth from simulated hail in mid-July or early August. Root yield and percent sucrose loss expressed as percent of the 0% simulate hail treatment increased as severity of the simulated hail event increased. Loss of root yield was greatest when simulated hail event occurred in July and was least in late August or calendar date closest to sugarbeet harvest. However, percent loss of sucrose was least in early July and greatest when simulated hail occurred in late August or calendar date closest to sugarbeet harvest. Finally, loss of root yield and sucrose was influenced by growing conditions following the simulated hail event, especially at the 75% simulated hail intensity. These outcomes tended to match results in the historical literature or from experiments conducted in the 1950 and 1960s with tetraploid and triploid varieties.
Effect of cover crop presence on herbicide efficacy.
WRIGHT, MADISON L.*1, RAM YADAV1, and ANDREW R. KNISS1, 1University of Wyoming, Plant Sciences, Dept. 3354, 1000 E. University Ave, Laramie, WY 82071.
Abstract View Article
Shade avoidance syndrome is a plant response to neighboring vegetation. It was hypothesized that cover crops could alter herbicide efficacy due to the relationship between shade avoidance syndrome and herbicide modes of action. Field experiments in sugar beet were conducted in 2023 and 2024 near Lingle, WY, using a split-plot randomized complete block design with four replicates in 2023 and three replicates in 2024. Winter wheat cover crop (presence or absence) was the whole-plot, and herbicide treatments were applied as the split-plot, allowing herbicide injury to be evaluated with and without the presence of wheat. The wheat cover crop was terminated at the sugar beet two true-leaf stage. The herbicides acifluorfen, clopyralid, and a pre-mix of phenmedipham + desmedipham + ethofumesate were applied in the sugar beet study at the 4 true-leaf stage. Weeds were removed by hand to exclude confounding effects of weed competition. In 2024, an additional field study was completed in Laramie, Wyoming to evaluate Chenopodium album and Bassia scoparia response to herbicide in the presence or absence of a cover crop. Herbicide treatments included basagran, dicamba, glufosinate, glyphosate, imazamox, and pyraflufen. Crop and weed injury were generally worse when cover crop and herbicides were combined, but the effect was additive in most cases rather than synergistic.
Stale seedbed for weed management in sugar beet.
TEYE-DOKU, NEWMAN B.*1, NEVIN LAWRENCE2, JENNA MEEKS1, RAMAWATAR YADAV1, and ANDREW R. KNISS1, 1University of Wyoming, Plant Sciences, Dept. 3354, 1000 E. University Ave, Laramie, WY 82071, 2University of Nebraska-Lincoln, Panhandle Research & Extension Center, 4502 Ave I, Scottsbluff, NE 69361.
Abstract View Article
Herbicides are widely used in North American sugar beet production due to the economic benefits and simplicity of weed control. Frequent exposure of weeds to herbicides has selected for herbicide-resistant weed species. Weeds in sugar beet fields are currently known to exhibit resistance to glyphosate, ALS-inhibitors, and dicamba, as well as other herbicides. Widespread herbicide resistance requires an integrated weed management program that reduces reliance on herbicides. Field studies were conducted near Lingle, WY and Scottsbluff, NE in 2023 and 2024 to evaluate the effectiveness of a stale seedbed approach to weed management in sugar beet. Treatments included four different sugar beet planting dates with 10 days interval between subsequent dates and three pre-emergence (PRE) herbicide treatments. In 2023, PRE herbicide treatments included ethofumesate, EPTC, or no PRE; while in 2024 herbicide treatments included metamitron + ethofumesate, a hand-weeded check and no PRE. Delaying planting date can effectively reduce mid-season density of some weed species could be an effective strategy to manage early emerging weeds in the absence of effective postemergence herbicides.
Mass trapping of adult sugar beet root maggot flies using pheromone-lure-baited bucket traps.
WENNINGER, ERIK J.* and BECK AKHIWU, University of Idaho, Kimberly Research & Extension Center, 3806 N 3600 E, Kimberly, ID 83341.
Abstract View Article
Larval feeding by the sugar beet root maggot (Tetanops myopaeformis) reduces yield in key sugar beet growing regions of North America. Management is heavily reliant on broad-spectrum insecticides that face growing threats from regulatory restrictions, underscoring the need to develop alternative management strategies. We first developed a new trap that optimized attraction of adult flies to visual and chemical cues, featuring a long-lasting pheromone lure and high capture capacity. Black bucket traps baited with polyethylene pheromone lures captured large numbers of female flies with little maintenance required. Next, field plot experiments were conducted in Idaho at four sites in 2023 and two sites in 2024. The study compared yield responses in a mass trapping treatment (i.e., plots surrounded by bucket traps) versus aldicarb-treated and non-treated check plots. Mass trapping and aldicarb treatments consistently performed similarly, both showing higher clean yield and estimated recoverable sucrose across most site-years of the study. Future studies should evaluate this approach at the whole field scale as well as determine the minimum number of traps required to achieve successful control. These findings demonstrate the potential for mass trapping of sugar beet root maggot as a new tool for management of this important pest of sugar beet.
Manure legacy effects on sugarbeet production and quality.
TARKALSON, DAVID D.*1, DAVID L. BJORNEBERG1, CHROSTOPHER W. ROGERS1 and ROBERT S. DUNGAN1, 1USDA-ARS, Northwest Irrigation and Soils Research Laboratory, Kimberly, ID 83341.
Abstract View Article
This long-term study assessed the effects of fertilizer and past manure applications on soil nutrient cycling and soil microbial function in the irrigated semiarid climate of southern Idaho. This data can help guide future nutrient inputs for subsequent crop production. In this paper we focus on crop (including sugarbeet) production and soil health indicators. From 2004 to 2009, solid dairy manure treatments were applied to plots at cumulative rates of 0, 134, and 237 dry Mg ha-1 (34-56 dry Mg ha-1 yr-1) in a randomized complete block with three replicates from 10 to 15 years post manure application, soil samples were taken from each treatment at 0-15 and 15-30 cm depth, and sugarbeet, corn, and barley yields and quality factors were determined. In general, the 237 Mg ha-1 treatment had greater yields compared to the 0 Mg ha-1 treatment. Many of the soil chemical and biological indicators were different between the manure and control treatments. In general, soil organic carbon (SOC) and biological indicators were significantly greater in the 134 Mg ha-1 and 237 Mg ha-1 treatments as compared to the 0 Mg ha-1. Manure had a legacy effect on crop and soil health factors up to at least 15 years.
Early season UAN applications in sugar beets.
REGITNIG, PETER J.* and JAY F. ANDERSON, Lantic Inc., 5405 64th Street, Taber, Alberta, T1G 2C4.
Abstract View Article
Urea ammonium nitrate (UAN) fertilizer can be applied in-season on commercial sugar beet fields in Alberta through irrigation pivots. If applications are made, they are generally applied to fully canopied sugar beets in mid-June to mid-July. There was interest in evaluating preemergence, at planting and early season postemergence applications of UAN to assess effects on plant stand, early season vigor and final root yield. Root yield was used as the primary harvest metric since any response in extractable sugar/acre in these trials was generally driven by root yield. UAN applications between 5 and 60 lbs N/acre in addition to recommended preplant incorporated (ppi) urea rates were included in 13 trials over an 11-year period between 2014 and 2024 to assess root yield gains. In-furrow UAN applications of 5 and 10 lbs N/acre significantly reduced sugar beet stand in a 2014 trial and because of this phytotoxicity these treatments were not included in further testing. In trials after 2014, UAN applications of 20 lbs N/acre or more were broadcast preemergence or postemergence using a plot sprayer followed by irrigation water or rainfall for incorporation. Three trials had statistically significant root yield increases averaging 2.2 tons/acre when the best UAN + ppi urea treatment was compared to a ppi urea alone treatment. Three trials had non-significant root yield increases averaging 1.9 tons/acre when the best UAN + ppi urea treatment was compared to ppi urea alone. A further 4 trials had non-significant root yield increases averaging 0.6 tons/acre when the best UAN + ppi urea treatment was compared to ppi urea alone. Spring UAN applications did not have any effect on sugar beet root yield in 3 trials. In 3 trials that included UAN treatments applied without ppi urea in nitrogen deficient conditions, root yield was significantly increased by an average of 1.7 tons/acre with a 20 lb N/acre UAN application and by an average of 3.0 tons/acre with a 40 lb N/acre UAN application compared to an unfertilized check. No injury was observed to sugar beets in 2 trials where a UAN rate of 25 lbs N/acre was mixed with 31 oz/acre glyphosate and applied postemergence. Trials over 11 years suggested there was a 23% chance of getting a significant root yield response to early season UAN applications and a further 23% chance of seeing a non-significant but notably positive root yield response.
Simulated hail timing and severity on sugarbeet root yield and sucrose content.
PETERS, THOMAS J.*1, ALEXA L. LYSTAD2 and MARK E. ZARNSTORFF3, 1North Dakota State University and the University of Minnesota, Fargo 58102, 2KayJay Ag Services, Inc., Horace, ND 58047,3National Crop Insurance Services, Overland Park, KS 66210.
Abstract View Article
An unintended outcome of crop production is losses due to abiotic factors including wind, water, and temperature. Defoliation of sugarbeet from hail can occur in any month throughout the growing season and can cause sugarbeet damage ranging from virtually undetectable damage to complete defoliation of vegetative plant material. Sugarbeet will almost always recover and continue their growth cycle unless hail causes stand loss which occurs mostly when sugarbeet are seedlings. Modern-day sugarbeet are diploid varieties as compared to tetraploid and triploid varieties characterized by heavy leaf texture and a large bush style plant architecture produced in previous decades. Experiments were conducted at multiple locations in 2021, 2022, and 2023 to measure vegetative index, root yield, and percent sucrose following simulated hail treatments at different growth stages and intensity with diploid varieties. Experiments were initiated in April and May when conditions were appropriate for cultivation by seeding at approximately 161,700 seed per hectare. Treatments were six sugarbeet rows spaced 56 cm apart and 9.1 m in length. Stands were thinned to approximately 180 sugarbeet per 30.5 m row in rows two through five when sugarbeet were at the 2-lf stage. Treatments were 0%, 25%, 50%, 75% and 100% (only petioles remaining) loss of canopy to simulate hail implemented by removing leaves from plants with hand shears in mid-July, early August and end of August. Health and density of the sugarbeet canopy was quantified approximately 21, 42, and 63 days after treatment (DAT) by collecting normalized difference vegetation index (NDVI) data with a RapidScan CS-45 active sensor (Holland Scientific, Lincoln, NE). Sugarbeet was defoliated and harvested mechanically in mid to late September from the center two rows of each plot and weighed. A 10-kg sample was collected from each plot and analyzed for sucrose content and sugar loss to molasses by American Crystal Sugar Company (East Grand Forks, ND). Sugarbeet canopy 21 days after the 25% and 50% canopy loss simulated hail treatment was greater than 90% of the 0% treatment at Crookston in 2022. All treatments were greater than 90% of the 0% treatment, 42 DAT. Further, canopy regrowth from simulated hail in late-August occurred at the same rate as canopy regrowth from simulated hail in mid-July or early August. Root yield and percent sucrose loss expressed as percent of the 0% simulate hail treatment increased as severity of the simulated hail event increased. Loss of root yield was greatest when simulated hail event occurred in July and was least in late August or calendar date closest to sugarbeet harvest. However, percent loss of sucrose was least in early July and greatest when simulated hail occurred in late August or calendar date closest to sugarbeet harvest. Finally, loss of root yield and sucrose was influenced by growing conditions following the simulated hail event, especially at the 75% simulated hail intensity. These outcomes tended to match results in the historical literature or from experiments conducted in the 1950 and 1960s with tetraploid and triploid varieties.
High nitrogen and variety performance.
OLSEN, DAVEY*1, ARIANNA HEGE1 and CRAIG FINDLAY1, 1Amalgamated Sugar-SBR Research, 911 W 8th Street Burley, Idaho 83318.
Abstract View Article
For many Idaho growers, annual manure applications are a necessary part of their farming system. This often results in high soil available nitrogen, frequently greater than 400 lb/A (3ft soil test). High nitrogen tends to lower sugarbeet sugar % and reduce sugar juice quality. These high nitrogen soils are problematic, and there are no established agronomic guidelines for how to optimize sugarbeet productivity on these soils. Anecdotally, growers report that some sugarbeet varieties perform better than others at high nitrogen levels. Perhaps variety selection provides the key to maximizing returns on these high nitrogen soils. Over two years we established field trials in Liberty, Idaho to evaluate the performance of commercial sugarbeet varieties under high nitrogen conditions (500 lb. N/A). Our results show that varieties performed differently relative to one another, and that some varieties could return high yields and good sugar % when grown under high nitrogen conditions. The interaction effect between sugarbeet variety and nitrogen level has broader implications for our industry and suggests that there is an optimal nitrogen level for each variety and that we may need to reconsider our one-rate-fits all approach to nitrogen recommendations.
Identification of volatile organic compounds as biomarkers for early detection of storage rots in sugar beets.
NKANPIRA, KELLY*1, MALICK BILL2, SHYAM L. KANDEL2, and EWUMBUA MONONO1, 1North Dakota State University, Department of Agriculture and Biosystems Engineering, Fargo, North Dakota, USA, 2USDA-ARS, Edward T. Schafer Agricultural Research Center, Fargo, North Dakota, USA.
Abstract View Article
Postharvest storage rots in sugar beets can result to significant sucrose losses during storage and in processing streams thus resulting in negative economic impact to the sugar industry in the U.S. Several pathogens including Penicillium and Leuconostoc species have been previously identified as important storage pathogens in beet piles in the U.S. Early detection of infection process of postharvest pathogens in sugar beet roots would be a promising approach to implement effective disease control strategies. This study aimed to profile the volatile organic compounds (VOC) released during the infection process of major storage pathogens in sugar beet roots. An in vitro assay was conducted to assess the VOC profiles of sugar beet root tissues inoculated with major storage pathogens including Penicillium expansum, Penicillium paneum and Leuconostoc mesenteroides. The VOCs were sampled on day 0, 3, 7 and 15 post inoculation at 30 ℃ using the solid-phase microextraction method and thereafter profiled using gas chromatography-mass spectrometry (GC-MS). The GC-MS analysis showed that ethanol and propanoic acid were the common VOCs in both uninoculated control and inoculated sugar beet root tissue samples. Penicillium expansum infections further resulted in increased amounts of ethanol compared to both P. paneum and Leuconostoc mesenteroides. Meanwhile, ethyl acetate was only observed in artificially inoculated sugar beet root tissues. Dimethyl ether and acetic acid were unique to P. expansum and P. paneum infected tissue. Our further studies using different sugarbeet cultivars are underway. Our ultimate goal is to develop the smart sensor platform to detect VOC biomarkers early on which will be helpful employing mitigation strategies to minimize the sucrose loss in postharvest sugar beets.
Nitrogen fertilizer management for Beta vulgaris in southern Minnesota.
METTLER, DAVID C.*1, MARK W. BLOOMQUIST1, and JOHN A. LAMB1, 1Southern Minnesota Beet Sugar Cooperative, 83550 County Road 21, Renville, MN 56284.
Abstract View Article
Nitrogen management is a priority for production of high-quality sugar beets. The use of nitrogen placement could offset the input cost of nitrogen and lower the overall use rate through more efficient use and availability. The objectives of these studies were to determine: 1) the optimal rate of spring broadcast urea, 2) if placement of UAN close to the row would improve sugar beet production over comparable broadcast urea rates, 3) evaluate sugar beet response to nitrogen rates following different previous crops, and 4) if biological products could improve nitrogen use efficiency. These trials were conducted as randomized complete blocks with four replications and repeated over three years (2022-2024) across two locations each year. To achieve the objectives of this study, the following treatments were used. Urea N applications of 30, 60, 90, 120, 150, and 180lbs of N were broadcast and incorporated in the spring at each location. UAN at a rate of 30 and 60lbs of N was applied in placements of 3” either side of the row x 1” deep and 3” either side of the row x 0” deep at planting time. Three sites were established following field corn, and three sites were established after soybean in the rotation. In this study, sites following field corn all responded to the application of additional nitrogen. Sites following soybean only responded to additional nitrogen one out of three years. Of the sites that responded to additional nitrogen, the placement of UAN closer to the row versus broadcast urea did not improve crop production and none of the biological products tested improved yield over the standard broadcast urea equivalent.
Evaluation of EcoRobotix green-on-green herbicide sprayer in sugarbeet.
LAWRENCE, NEVIN C.*1 and KNISS, ANDREW R.2, 1University of Nebraska, 4502 Ave I, Scottsbluff, NE 69361, 2University of Wyoming, Dept. 3354, 1000 E University Ave. Laramie, WY 82071.
Abstract View Article
Green-on-green sprayer technology allows an herbicide to be applied only to weeds while avoiding contact with the crop. This technology may allow use of herbicides that normally would cause severe injury or crop loss while controlling herbicide-resistant or herbicide-tolerant weeds within the same field, potentially using less herbicide per-hectare compared to a traditional broadcast application. In 2024, a series of studies were conducted to determine potential herbicides for use within in sugarbeet including high concentrations of urea, acetic acid, sulfuric acid, phenmedipham, desmedipham, and standard use rates of ammonium nonanoate and glufosinate on herbicide-resistant kochia. A later field trial was conducted to test urea, ammonium nonanoate, and glufosinate with the EcoRobotix sprayer when applied at the cotyledon, two-true leaf, and four-true leaf growth stage. Crop safety was excellent with no loss of stand or observed sugar beet injury. Weed control was also excellent with ammonium nonanoate and glufosinate, however coverage decreased as sugarbeet increased in size. Green-on-green spray technology can effectively manage herbicide-resistant weeds in sugarbeet when applied early in the season but should be paired with soil active herbicides and other weed control practices to maximize weed control and crop safety.
Potassium and nitrogen applications for sugarbeet yield and quality in Southern Minnesota and Imperial Valley of California.
LAMB, JOHN A.*1, DAVID C. METTLER2, and MARK W. BLOOMQUIST2, 1University of Minnesota, 2182 Woodbridge Way, Woodbury, MN 55125, 2Southern Minnesota Beet Sugar Cooperative, 83550 County Road 21, Renville, MN 56284.
Abstract View Article
The use of K and N fertilizer for sugar beet growth and quality have been a concern in the sugar beet growing areas of Southern Minnesota and California for several years. Concerns include the need for optimum root yield and the effect of K and N on quality. Both nutrients are impurities for the extraction of sucrose from the beet root. The objective of these studies was to assess and compare the effects of K and N in high soil test K soils of the Imperial Valley (IV) and the Southern Minnesota Beet Sugar Cooperative (SMBSC) growing areas. Three studies were conducted. The first in the SMBSC growing area had six sites from 2010 to 2012. The treatments included a factorial arrangement of four N rates and six K rates. The second study was conducted in the IV growing area had three sites from 2013 to 2015 with four N rates and six K rates. The final study was conducted from 2021 to 2023 in the SMBSC growing area with three N rates and five K rates. Comparing the results of the two SMBSC studies, in the 2010 to 2012 study, K application increased root yield and quality at 50 % of the sites with no quality issues from the excessive K application rates. Nitrogen application increased root yield at 50% of the sites and decreased quality at 75 % of the sites. In the 2021 to 2023 SMBSC study, K application did not affect root yield or quality, and N application reduced quality at 67 % of the sites. In the IV study, there was little interaction between N and K for root yield and quality. Excess application of K did not reduce sugar beet quality. In general, the excess application of K did not affect the quality in any of the studies.
Forty years of kochia (Bassia scoparia) control with herbicides in sugar beet.
KNISS, ANDREW R.*, University of Wyoming, Plant Sciences, Dept. 3354, 1000 E.
University Ave, Laramie, WY 82071.
Abstract View Article
Kochia remains a problematic weed in sugar beet, especially in the Western United States.
Kochia has evolved resistance to ALS-inhibiting herbicides, PPO-inhibiting herbicides, triazine herbicides, synthetic auxin herbicides including dicamba, and glyphosate. In an
effort to manage this weed, older herbicides have received new interest. Annual herbicide
efficacy reports starting in 1984 were reviewed and summarized to provide insight into the
history of kochia control in sugar beet.
Low concentration of chlorine dioxide gas reduced invert sugars and raffinose accumulation in postharvest sugarbeet roots.
KANDEL, SHYAM L.*1, SANDESH DANGI1,2, GRANT HERGES1, and DAVID J. SMITH1,
1USDA-ARS, Edward T. Schafer Agricultural Research Center, Fargo, ND 58102,
2North Dakota State University, Department of Plant Pathology, Fargo, ND, 58102.
Abstract View Article
Sugarbeet roots are susceptible to postharvest storage diseases in storage piles. During
infection, postharvest pathogens metabolize sucrose into invert sugars (i.e., glucose and
fructose), raffinose, and other carbohydrate impurities which interfere with the sugar refinery
process. Chlorine dioxide gas (ClO2) is an effective antimicrobial agent commonly
used to disinfect surfaces in some industrial and agricultural applications. In this study, we
hypothesized that low concentrations of ClO2 gas would improve the postharvest storage
quality of sugarbeet roots and limit the formation of invert sugars and raffinose in treated
roots. Sugarbeet roots were stored for 7 weeks at 5°C and treated with dry granules of
sodium chlorite compound with an acid activator sachet (ICA TriNova; Marietta, GA)
aiming to generate approximately at 0, 25, 50, 75, and 100 mg of ClO2 per kilogram of
sugarbeet roots. Treatment had no significant effect on sucrose content (P > 0.05). But
invert sugars decreased significantly (P < 0.05), when the roots were treated with ClO2
media when compared to the controls across all levels of ClO2. Raffinose accumulation
was also numerically reduced in the sugarbeet roots across all levels of ClO2, however
significant (P < 0.05) reductions occurred in roots treated with 75 and 100 mg of ClO2
kg-1 root. The experiment was repeated in 2024 with slightly aged roots and carbohydrate
analysis is underway. Sugarbeet roots treated with all levels of ClO2 had no visible signs
of storage disease in both years. Our data suggest that use of ClO2 might be a viable option
to minimize storage disease and improve storage quality of sugarbeet roots.
Methods of collecting temperature of sugar beet storage piles at Southern Minnesota Beet Sugar Cooperative for research purposes.
GROEN, CODY J.*, Southern Minnesota Beet Sugar Cooperative, 83550 County
Road 21, Renville, MN 56284.
Abstract View Article
Researchers at Southern Minnesota Beet Sugar Cooperative (SMBSC) have utilized various
methods for determining storability of sugarbeets. Temperature of sugarbeet piles has
been used as an indicator of how well sugarbeets are storing. SMBSC researchers have
developed a robust set of techniques that can be used to accurately track temperature at
various depths of sugarbeet piles. The objective of this presentation is to share the techniques
used by SMBSC researchers, the development of the techniques, and the beneficial
and detrimental aspects of each.
Storage conditions and plant genetics affect sugar transporter gene expression with likely effects on postharvest sucrose losses.
FUGATE, KAREN K.*1, JOHN D. EIDE1, and FERNANDO L. FINGER2, 1USDA-ARS,
Edward T. Schafer Agricultural Research Center, Fargo, ND 58102; 2Universidade
Federal de Viçosa, Departamento de Agronomia, Viçosa, Brazil.
Abstract View Article
Sugar transporters effect movement of sugars across cellular membranes and play a critical
role in relocalizing carbon substrates within cells, tissues, and organs to support their metabolism.
Sugar transporters are especially important for postharvest sugarbeet roots since
the export of sucrose from the vacuole of parenchymal storage cells not only fuels root
metabolism but also drives sucrose loss during storage. Despite the obvious importance of
sugar transporters to sugarbeet root postharvest metabolism, the identity and expression of
sugarbeet root sugar transporters during storage have never been examined. Therefore, the
expression of sugar transporters in harvested and stored sugarbeet roots was determined with
respect to storage duration and temperature and in genotypes that likely differed in rates of
postharvest sucrose utilization due to differences in respiration rate. Highly and differentially
expressed sugar transporters largely belonged to the SWEET (sugars will eventually be
exported transporters) and TST (tonoplast sugar transporters) classes of sugar transporters.
Eight SWEET genes and two TST genes were expressed in postharvest sugarbeet roots with
expression of SWEET and TST genes generally increasing with time in storage, but only
minimally affected by storage temperature. SWEET N3 and TST 1 were the most highly expressed
and upregulated sugar transporter genes during storage and were also differentially
expressed in lines with genetic differences in storage respiration rate. Overall, these results
highlight the likely importance of SWEET and TST genes for postharvest sugarbeet root
metabolism and identify gene candidates that may have roles in storage sucrose loss.
United Beet Seeds (UBS): A joint Venture that forms a strong and reliable player in the sugar beet industry.
ESCALON, LILIAN*1, NIKLAS ANDERSSON 1, and HENDRIK TSCHOEP1,
1United Beet Seeds, Industriepark 15, 3300 Tienen, Belgium.
Abstract View Article
On September 17, 2024, DLF Seeds A/S and Groupe Florimond Desprez announced the
formation of United Beet Seeds (UBS), a new joint venture that brings together the sugar
beet, fodder beet, and industrial chicory seed businesses, along with their R&D activities.
This strategic partnership will position UBS as a leading player in the international beet
seed market. By joining forces, UBS aims to contribute to the evolving beet seed industry
in a way that balances the demands of growers, the environment, and the market. The sugar
beet sector is currently facing new challenges, driven by legislative changes, the need for
climate resilience, and advances in technology. Recognizing the importance of working
together to overcome these challenges, UBS combines the knowledge and experience of its
founding partners to offer innovative yet practical solutions. At the heart of UBS’s mission
is a commitment to research, sustainability, and continuous improvement, all focused on
supporting farmers and the broader industry as they adapt to agro-ecological changes. This
collaboration reflects a shared belief that sustainable progress in agriculture is possible
through partnership, dedication, and investment in innovation. We are excited to explore
the journey ahead and invite you to join us in learning more about our vision, strategic
priorities, and how UBS aims to contribute to the future of the beet and chicory industry.
Advances in sugar beet storage systems in Nordic countries: A decade of research and development.
EKELÖF, JOAKIM*1, 1Nordic Beet Research Foundation, Borgeby Slottsväg 11, SE
– 237 91 Bjärred.
Abstract View Article
This presentation offers a comprehensive overview of the advancements in sugar beet
storage systems in Nordic countries over the past decade. Focused on enhancing the efficiency
and sustainability of sugar beet storage, the research evaluates both the physical
and chemical properties of various sugar beet varieties from a storage perspective. Notable
innovations include the development of quick tests to evaluate storability, which
significantly improve the monitoring and maintenance of beet quality during storage. Additionally,
the presentation covers the setup of large-scale temperature monitoring systems
for clamps, which ensures a full overview of temperatures and improves decision-making
to prevent spoilage and preserve beet quality. Research into the frost tolerance of beet
material is also presented, emphasizing the importance of selecting the correct varieties
and management techniques to withstand cold temperatures. Furthermore, the practical
evolution of clamp covering techniques is discussed, with improvements designed to better
protect sugar beets from adverse weather conditions and minimize losses. Looking forward,
the presentation outlines future strategies and plans for optimizing storage systems,
ensuring continued progress and adaptation to climate challenges. This work underscores
the critical role of innovative storage practices in the sugar beet industry, contributing to
sustainability and economic viability in the region.
Effects of post-harvest cold storage in bolting and disease susceptibility (Botrytis cinerea and Fusarium gramminearum) in sugar beet roots.
BUITRAGO-ACOSTA, MARIA C.*1 and RACHEL P. NAEGELE1,2, 1Michigan State
University, Department of Plant, Soil, and Microbial Sciences, 1066 Bogue St, East
Lansing, MI 48824, USA, 2USDA-ARS Sugarbeet and Bean Research Unit, Michigan
State University, 1066 Bogue St., East Lansing, MI 48824, USA.
Abstract View Article
Two traits that could enhance sugar beet production are bolting tolerance and disease tolerance/
resistance to post-harvest storage pathogens. Bolting refers to the elongation of
the stem usually followed by flowering; sugar beets are biennial, requiring vernalization
(prolonged cold period) to induce bolting. Improving bolting tolerance in sugar beet roots
could prolong the growing time of sugar beets, make winter beets a more viable option for
the industry and increase sucrose content. Sucrose losses caused by post-harvest storage
pathogens can be significant, particularly in areas where storage piles are unable to remain
frozen. Developing tolerant/resistant sugar beets to post-harvest storage pathogens could
increase sugar recovery during prolonged storage. To understand the effect of cold storage
in sugar beet roots, we (1) phenotypically characterized bolting and disease susceptibility
for eleven genotypes from the USDA-MSU germplasm across variable durations of cold
treatment at 4°C and (2) analyzed the transcriptomic changes for three of those genotypes
across cold durations of 0, 4, 8, 13 and 17 weeks. We observed differences in bolting response
between genotypes and within plants of each genotype. The number of bolting
plants increased as cold storage time increased, and the time between planting and bolting
decreased as cold storage period increased. For the disease susceptibility, we observed larger
lesions caused by B. cinerea compared to F. gramminearum. Subtle differences in lesion
diameter were noted across genotypes for both pathogens. Larger lesion diameter caused
by B. cinerea was noted as cold storage period increased across genotypes. Differences in
transcriptomic response associated with bolting and disease susceptibility were detected.
Artificial intelligence-based insect traps for monitoring sugarbeet root maggot fly activity.
BOETEL, MARK*1, JACOB RIKHUS1, and REED THOMA1, 1North Dakota State University,
School of Natural Resource Sciences, Dept. 7650, P.O. Box 6050, Fargo, ND
58108-6050.
Abstract View Article
The sugarbeet root maggot (SBRM), Tetanops myopaeformis (Röder) is a significant economic pest of sugarbeet in the Red River Valley growing area. It is also an important pest of the crop in several other states, and in the Canadian province of Alberta. Producers and pest management advisors have relied on manual sticky-stake traps or similar technology to monitor SBRM fly activity in commercial sugarbeet fields for several decades. However, artificial intelligence (AI) technology has the potential of improving the practice of insect pest monitoring. This technology involves the creation of a teachable machine (e.g., computer program and associated hardware) that is trained to work or react in response to information like a human being. The machine “learns” to react to specific information patterns and then performs a response function controlled by an algorithm. The machine continues to learn based on feedback into the algorithm, which can substantially improve its performance. This experiment involved field testing of two AI-based insect traps for monitoring sugarbeet root maggot fly activity in the Red River Valley during the 2018, 2019, and 2020 growing seasons. The following traps were compared for accuracy and efficiency with conventional sticky-stake traps: 1) the DTN SMART Trap, a modified Delta insect trap equipped with a high-definition camera mounted in its roof that collected images of insects captured on a sticky card placed inside the floor of the trap housing; and 2) the DTN Z-trap, which functioned as a low-voltage “bug zapper”, that counted SBRM flies based on electrical impedance produced by their bodies making contact with its electrodes. Both traps were equipped with cellular-enabled mobile reporting technology integrated with a data storage website and mobile device app, which collectively allowed for real time data access. Preliminary results from processed samples indicate that the camera-equipped SMART Trap exceeded 80% accuracy (before providing the algorithm with feedback), whereas the Z-trap tended to significantly overestimate counts as a result of flies in catch containers recovering from shock, repeatedly landing back on electrodes, and being recounted. That issue was resolved by adding a killing agent to the catch container in subsequent testing. Although sample processing and data compilation are ongoing, findings thus far suggest that this technology has strong potential to revolutionize the process of SBRM monitoring in sugarbeet, and it may also have applications in monitoring other important crop insect pests.
Reducing late rot in Imperial Valley sugar beet production using variety tolerance and irrigation.
BLOOMQUIST, MARK W.*1, JOAQUIN SANTIAGO2, and JOHN LAMB3. 1Southern
Minnesota Beet Sugar Cooperative, 83550 County Road 21, Renville, MN 56284,
2Spreckels Sugar, 395 West Keystone Road, Brawley, CA 92227, 3University of Minnesota,
2182 Woodbridge Way, Woodbury, MN 55125.
Abstract View Article
Sugar beet is harvested in the Imperial Valley of California from April through July. Daily
high temperatures often exceed 110-115 degrees F in late June and July. The extreme heat,
in combination with saturated soil conditions due to furrow irrigation, creates favorable
conditions for the development of late rot in sugar beet fields. Late rot is a complex of two
or more pathogens that can rapidly deteriorate a sugar beet root. Pythium aphanidermatum
and Phytophthora drechsleri are the two primary soil-borne pathogens contributing to late
root rot losses in fields. Late rot complex reduces the sucrose and purity content of the
sugar beets, which leads to processing difficulties and losses. Imperial Valley sugar beet
varieties have varied tolerance to late rot infection. Furrow irrigation is the predominant
irrigation type for sugar beet production in the Imperial Valley. Trials were established
to explore if the combination of variety tolerance and irrigation type can reduce late rot
development and increase yields during the final month of the harvest season. Trials were
established during the 2022-2023 and 2023-2024 growing seasons at the Imperial Valley
Research Center near Brawley, CA. The trials were randomized complete block designs
with a split-plot arrangement. Irrigation type (sprinkler vs. furrow) was the whole plot,
and variety (tolerant vs. susceptible) was the subplot. The trials were planted in early October
and harvested in early August. In both seasons, root yield and extractable sucrose per
acre were greater, and the percent rot was lower with the use of a tolerant variety. Sucrose
content was greater with the use of sprinkler irrigation in both seasons. The trial is being
repeated for the 2024-2025 growing season.
How does simulated hail damage timing affect sugar beet root yield and quality?
ANDERSON, JAY F.*1 and PETER J. REGITNIG1, 1Lantic Inc., 5405 64th Street, Taber,
Alberta, T1G 2C4.
Abstract View Article
Hail damage can cause significant root yield and quality reductions to a sugar beet crop.
In southern Alberta, damaging hailstorms usually occur in July and August and can sometimes
result in 100% defoliation, depending on storm intensity and duration. The objective
of this study was to determine how simulated hail damage treatments affected sugar beet
root yield and quality at harvest time. This test was conducted for 4 years during the 2019-
2022 growing seasons. Simulated hail damage treatments of 100% defoliation were applied
on four dates in July and four dates in August and were compared to an untreated check to
measure reductions. Treatments were applied using a 4-row single drum defoliator starting
on July 7 and were continued every 7 days until the end of August. At harvest, the center
2 rows of each test plot were lifted and weighed in the field. A subsample of 10-12 sugar
beets per plot were bagged and analyzed for sugar content and impurity levels. It was determined
that July hail simulation timings reduced root yield by 36.3% on average compared
to a 21.2% reduction for August hail simulation timings. Visual observations following
hail simulation treatments indicated that July defoliation timings had quicker, and fuller
regrowth compared to August timings. August hail simulation timings reduced extractable
sugar per ton (EST) by 19.6% compared to only an 8.2% reduction for July timings. Treatment
effect on percent sugar was very similar to EST response. Reductions in extractable
sugar per acre (ESA) increased gradually from the first hail simulation treatment and
reached a maximum reduction of 46% on July 28 before reductions started to decrease until
the final treatment date in late August. Late July to early August treatment timings affected
loss to molasses more than treatments outside of that date range. It was concluded that the
latest hail simulation timing in July caused the most significant damage overall, as indicated
by losses in ESA, and resulted in the worst possible outcome for producers.
Evaluation of metamitron safety and weed control based on 2024 Emergency Use Label.
AKUOKO, ABRAHAM*1, ANDREW R. KNISS2, and NEVIN C. LAWRENCE3, 1University
of Nebraska, 1400 R St, Lincoln, NE 68588, 2University of Wyoming, Dept.
3354, 1000 E University Ave. Laramie, WY 82071, 3University of Nebraska, 4502 Ave
I, Scottsbluff, NE 69361.
Abstract View Article
Metamitron, a Group 5 herbicide, was granted an emergency use label for 2024 in 10 and 14 counties in Colorado and Nebraska, respectively, for control of herbicide-resistant (HR) Palmer amaranth (Amaranthus palmeri) in sugar beet. The 2024 emergency use label, however, was restrictive, allowing sugar beet growers to only apply 3.27 kg ai ha-1 before sugar beet emergence. In situations where sugar beet growers miss a PRE application before HR-Palmer amaranth emergence, there is no option for HR-Palmer amaranth control before the 2TL stage. In 2024, studies were conducted in Scottsbluff, NE, to evaluate weed control efficacy under the terms of the 2024 label. Common lambsquarters (Chenopodium album) control was excellent with the use of metamitron compared with the non-treated check and ethofumesate. HR-Palmer amaranth was controlled with metamitron compared with the non-treated check. In a later study, sugar beet safety was assessed when herbicide is applied off-label. Weed control did not improve when metamitron was applied at the cotyledon stage; however, control was excellent when followed up with metamitron + ethofumesate + s-metolachlor at the 2 TL. Crop safety was excellent with no noticeable injury and decrease in crop stand. Metamitron can effectively manage HR-weeds in sugar beets but should be followed up with Group 15 herbicide to maximize efficacy.
Kochia control with Phenmedipham in sugarbeet.
ABERLE, ADAM D.*1 and THOMAS J. PETERS1, 1North Dakota State University and
University of Minnesota, NDSU, Dept. 7670, PO Box 6050, Fargo, ND 58108-6050.
Abstract View Article
Sugarbeet growers in Minnesota and North Dakota face significant challenges with glyphosate-
resistant (GR) weeds, including kochia (Bassia scoparia L.). Greenhouse and
field experiments to evaluate phenmedipham for kochia control have been ongoing since
2022. A 24(c) local needs label allows for phenmedipham (brand name Spin-Aid®) use
alone or in tank-mixtures for kochia, common lambsquarters, and common ragweed control
in sugarbeet. Sugarbeet visible growth reduction injury was greatest 14 to 21 days
after application and increased as the number of Spin-Aid applications increased from
one to three in the greenhouse and in field experiments. Injury was less after 21 days.
Injury also increased in the greenhouse when Spin-Aid application coincided with air
temperature greater than 24 C or when Spin-Aid was mixed with S-metolachlor. Two- or
three-times Spin-Aid application or Spin-Aid following ethofumesate reduced root yield
but did not affect sucrose content or recoverable sucrose per hectare. Kochia control from
Spin-Aid was dependent on kochia size, tank-mixture partner, or environmental conditions
at application. In the greenhouse, 3-times Spin-Aid application at 182, 273, and 363
g ai ha-1 with ethofumesate and methylated seed oil improved kochia control as compared
to 2-times Spin-Aid application at 182 and 363 g ha-1 with ethofumesate and methylated
seed oil. In the field, kochia control improved with 3-times Spin-Aid application as compared
to a single or 2-times Spin-Aid application. Application rate did not seem to matter
as much as multiple applications timed 5 to 7 days apart. Spin-Aid was mixed with ethofumesate
or ethofumesate and glyphosate with high surfactant methylated seed oil. Kochia
control was improved when Spin-Aid followed ethofumesate preemergence (PRE).
Future research will focus on optimizing Spin-Aid rate with environmental conditions. We
may also consider a 4-times Spin-Aid application.
Agronomy - Poster
Sugarbeet processing precipitated calcium carbonate lime effects on a crop rotation and soil properties: sites 2 and 3.
TARKALSON, DAVID D.*1, DAVID L. BJORNEBERG1, OLIVER T. NEHER2, DAVEY OLSEN2 and GREG DEAN2, 1USDA-ARS Northwest Soils and Irrigation Research Laboratory, Kimberly, ID, 2Amalgamated Sugar Company, Boise, ID.
Abstract View Article
Each year in Idaho and Oregon, 351,000 Mg of precipitated calcium carbonate (PCC) (a byproduct of sucrose extraction from sugarbeet) is produced and stockpiled at sugarbeet processing factories. Currently there are limited disposal strategies for the PCC and these stockpiles continue to grow over time. The simplest solution would be to apply the PCC directly to agricultural fields each year, however the effects of PCC on high pH soils and crop rotations in the growing area are not well understood and growers are understandably hesitant. Two studies were conducted at the USDA-ARS laboratory in Kimberly, ID to determine the effects of PCC application to a high pH silt loam soil on a sugarbeet, dry bean and barley rotation and soil properties. For each study, three PCC treatments (rate and timing) and an untreated control were evaluated. The PCC had no effects on crop yields and most soil properties. The only common effect of PCC treatments was an increase in soil phosphorus (P) concentrations compared to the control, indicating the PCC can serve as a P fertilizer. For all three crops in this study, PCC was applied at rates that resulted in applied P rates that were 1.6 to 5.3 times greater than even the highest published recommended agronomic P rates. Compared to the control, bicarbonate soil P concentrations increased by 139% and 84% when PCC was applied at a rate of 87.9 Mg ha-1 in Study1 and Study 2, respectively. The PCC used in this study can safely be applied at rates up to 87.9 Mg ha-1 to heavier textured alkaline soils in the local growing area. Disposing of PCC in this way represents a viable strategy for reducing PCC stockpiles.
Evaluation of root firmness and cell wall components in sugarbeet lines for reducing sugar loss during post-harvest piling storage.
ADDINGTON, JOLENE*1, AHMET BARKAN BOSTAN1, LISA PREISTER2, KAREN FUGATE2, NATHAN WYATT2, MELVIN BOLTON2, XUEHUI LI1 and CHENGGEN CHU*2, 1North Dakota State University, Department of Plant Sciences, Fargo, ND 58102, 2USDA-ARS, Edward T. Schafer Agricultural Research Center, Sugarbeet Research Unit, Fargo, ND 58102.
Abstract View Article
Roots of sugarbeet (Beta vulgaris L. ssp. vulgaris Doell.) are easily injured during harvesting or making piles for storage prior to processing in sugar factory, which not only increases the risk of roots being infected by pathogens to cause root rot and affecting sucrose extraction, but also enhances respiration of root tissue to provide energy for wound healing and causing significant sugar loss. Increase root firmness to reduce root injuries during harvesting and post-harvest piling storage will provide a solution to reduce post-harvest sugar loss. Root tissue firmness is determined by strength of tissue cells and cell wall structure could be a key determinant of tissue texture. Analyzing content of pectin types (mainly water-soluble pectin, sodium-carbonate-soluble pectin and chelator-soluble pectin) in cell wall will provide critical information of increasing root firmness via breeding selection focuses on cell wall components. In this study, we will evaluate root tissue firmness and cell wall components in 360 sugarbeet genotypes to find out the relationship between root firmness and cell wall pectin compositions before and after roots were cold stored for three months. A genome-wide association study (GWAS) will be conducted to identify genomic regions associated with root firmness and cell wall pectin components to develop markers linked to genes that increase root hardness. This research will identify genetic sources and develop markers for efficiently creating new germplasm with enhanced root hardness towards reducing sugar loss during harvesting and post-harvest piling storage.
Sugarbeet processing precipitated calcium carbonate lime effects on a crop rotation and soil properties: sites 2 and 3.
TARKALSON, DAVID D.*1, DAVID L. BJORNEBERG1, OLIVER T. NEHER2, DAVEY OLSEN2 and GREG DEAN2, 1USDA-ARS Northwest Soils and Irrigation Research Laboratory, Kimberly, ID, 2Amalgamated Sugar Company, Boise, ID.
Abstract View Article
Each year in Idaho and Oregon, 351,000 Mg of precipitated calcium carbonate (PCC) (a byproduct of sucrose extraction from sugarbeet) is produced and stockpiled at sugarbeet processing factories. Currently there are limited disposal strategies for the PCC and these stockpiles continue to grow over time. The simplest solution would be to apply the PCC directly to agricultural fields each year, however the effects of PCC on high pH soils and crop rotations in the growing area are not well understood and growers are understandably hesitant. Two studies were conducted at the USDA-ARS laboratory in Kimberly, ID to determine the effects of PCC application to a high pH silt loam soil on a sugarbeet, dry bean and barley rotation and soil properties. For each study, three PCC treatments (rate and timing) and an untreated control were evaluated. The PCC had no effects on crop yields and most soil properties. The only common effect of PCC treatments was an increase in soil phosphorus (P) concentrations compared to the control, indicating the PCC can serve as a P fertilizer. For all three crops in this study, PCC was applied at rates that resulted in applied P rates that were 1.6 to 5.3 times greater than even the highest published recommended agronomic P rates. Compared to the control, bicarbonate soil P concentrations increased by 139% and 84% when PCC was applied at a rate of 87.9 Mg ha-1 in Study1 and Study 2, respectively. The PCC used in this study can safely be applied at rates up to 87.9 Mg ha-1 to heavier textured alkaline soils in the local growing area. Disposing of PCC in this way represents a viable strategy for reducing PCC stockpiles.
Do harvest-aids improve sugarbeet topping?
WISHOWSKI, DAVID*1 and CHRISTY L. SPRAGUE2, 1Michigan State University Extension and Sugarbeet Advancement,2 Michigan State University, Department of Plant, Soil, and Microbial Sciences, 1066 Bogue Street, East Lansing, MI 48824.
Abstract View Article
Effectively topping sugarbeets during early harvest has been a struggle for Michigan sugarbeet growers. To address this concern various products were applied as harvest-aids 6 d prior to topping in early September 2024. Products used as harvest-aids were sodium chlorate (Defol 5) at 7.8 and 15.6 kg ha, calcium (Max-In Calcium) at 1.2 kg ha, paraquat (Gramoxone) at 0.55 kg ha, saflufenacil (Sharpen) at 50 g ha, diquat (Reglone) at 0.56 kg ha, and a paraquat + saflufenacil tank-mixture. Sodium chlorate and saflufenacil treatments were applied with methylated seed oil and a non-ionic surfactant was used with diquat and paraquat alone treatments. Leaf desiccation was evaluated 2 and 6 d after treatment (DAT) on a scale from 0 to 100%. Sugarbeet leaf regrowth was also evaluated. Sugarbeets were topped, and leaf removal was evaluated. Roots were sub-sampled and analyzed for purity, % sugar, and recoverable white sugar per kg (RWSkg) of root tissue. Sugarbeet leaf desiccation was the greatest with diquat (84%), 2 DAT. Desiccation with paraquat alone and with saflufenacil was ~75%, 2 DAT. All other treatment provided less than 10% leaf desiccation. By 6 DAT, leaf desiccation with diquat and paraquat treatments were similar and sodium chlorate leaf desiccation was 32 and 42% with the low and high rates, respectively. Calcium had virtually no visual effect on leaf tissue and saflufenacil only resulted in slight leaf speckling (12%). There was also 17-20% leaf regrowth with the diquat and paraquat treatments. No treatments improved the effectiveness of leaf removal from topping compared with the untreated control. In fact, leaf removal was significantly lower (10% or more) when leaf desiccation was high, diquat and paraquat treatments. All preharvest treatments with the exception of saflufenacil and calcium reduced RWSkg by 8% or more compared with the untreated control. In conclusion, the use of preharvest treatments were not effective in improving sugarbeet topping and other techniques or system changes will likely be needed to improve leaf removal from topping operations during early harvest.
The case for static range nitrogen management in Northwest U.S. sugarbeet production.
TARKALSON, DAVID D.*1, DAVEY OLSEN2, DAVID L. BJORNEBERG1 and CHRISTOPHER W. ROGERS1, 1USDA-ARS Northwest Soils and Irrigation Research Laboratory, Kimberly, ID, 2 Amalgamated Sugar Company, Boise, ID.
Abstract View Article
Nitrogen (N) management is important in sugarbeet (Beta vulgaris) production because it affects yield and quality. As Northwest U.S. sugarbeet yields continued to increase over the preceding decades, crop response data suggested that the established and utilized yield goal N management (YGNM) method for determining N requirements was leading to the over application of N fertilizer. This paper evaluates N supply effects on sugarbeet yields from three studies (26 research site-years) from 2005 to 2021. The use of an alternate static range N management (SRNM) approach was compared to the historically used YGNM approach. Historical data (1977-2021) shows that the N supply needed to produce maximum yields is within a narrow range. The N supply range required to produce maximum root and sucrose yields was 197 to 231 kg N ha-1. Our analysis shows that over time the YGNM approach has increasingly over recommended N supply. At the 12 responsive site-years, the YGNM N recommendations supplied an average of 61 kg N ha-1 more than needed to maximize yield. Over 80% of fields in the Amalgamated Sugar Company (ASCO) growing area are over supplying N relative to the SRNM range. Data suggests N supply affects sugarbeet quality factors to a lesser degree than historically thought. Nitrogen supply did not affect root yields in 14 of the 26 site-years, likely a result of high in season N mineralization and other microbiome effects. We recommend that SRNM (200 to 230 kg N ha-1) replace YGNM for sugarbeet production in the Northwest U.S.
Variations in stand counts affecting sugar beet yield and quality.
SEARLE, AARON*, GREG DEAN, LANCE PITCHER, ZACH LUEKENGA and SKYLEN HAWKENS, Amalgamated Sugar Company, Nampa, ID 83686.
Abstract View Article
From 2012 to 2014, agronomists with Amalgamated Sugar Company compared different plant populations and planting times and the effects on sugar beet yield and quality. Plant populations ranged from 55 to 255 beets per 100 ft of row in 40 beet increments; however, to assess the economic impact of different stand counts, the spread was too wide. In 2024, a study with populations ranging from 65, 70, 75, 80, 95 and 150 beets per 100 ft was initiated to provide further granularity. Plots were established by overseeding and thinning to a prescribed stand. Four-row plots were planted April 3, 2024, and the study area was managed according to standard grower practices and irrigated using drip-irrigation to maximize uniformity. On October 4, plots were harvested using a two-row harvester and two 25 lbs samples were for quality analysis.
Significant differences were only observed for estimated recoverable sucrose (lbs/A) between lowest (65 beets / 100 ft) and highest (150 beets / 100 ft) stands. However, as expected there were numerical differences between treatments for tons per acre.
Reevaluating common ragweed control in sugarbeet in Minnesota and North Dakota.
PETERS, THOMAS J.* and ADAM D. ABERLE, North Dakota State University and the University of Minnesota, NDSU, Dept. 7670, PO Box 6050, Fargo, ND 58108-6050.
Abstract View Article
Glyphosate resistant (GR) weeds dominate sugarbeet production in Minnesota and North Dakota. Sugarbeet growers attending the 2024 grower seminars categorized GR waterhemp [Amaranthus tuberculatus (Moq.) J. D. Sauer] as their most important weed control challenge on 61% of the sugarbeet hectares in Minnesota and North Dakota. GR common ragweed (Ambrosia artemisiifolia L.) was identified on 7% of sugarbeet hectares but was recognized as their most important weed control challenge on 29% of hectares in Traill and Grand Forks counties in North Dakota and Polk, Norman, and Marshall Counties in Minnesota. In 2024, ragweed may have escaped clopyralid application since below normal soil temperatures extended ragweed germination and emergence into June and early July. Two times clopyralid at 66-79 g ha-1 in combination with glyphosate on common ragweed less than 5 cm (2 lf sugarbeet) and 14 days later is the most frequent control program. Clopyralid must be applied before sugarbeets reach the 8 lf stage or approximately end of May in Minnesota and North Dakota. Common ragweed emerges in late April and May. Common ragweed must undergo a dormant period from late fall through winter before germinating the following year. Increasing air temperatures promote germination, but soil temperatures above 30C will halt germination and send the seeds back to dormancy until repeating the cold requirement the following winter. There are other accounts for ragweed escapes including biotypes more clopyralid tolerant. The objectives of our research were to improve common ragweed control from clopyralid and to extend control further into the season. Experiments were conducted with sugarbeet grower cooperators in Norman Counties in 2023 and 2024. Herbicides were applied POST with a bicycle wheel plot sprayer with a shielded boom to reduce particle drift and calibrated to deliver 159 L ha−1 through 8002XR nozzles (XR TeeJet® Flat Fan Spray Tips, TeeJet® Technologies, Glendale Heights, IL) spaced 51 cm apart and pressurized with CO2 at 207 kPa. Evaluations were a visible assessment of control by comparing the treated area in the plot to the bordering non-treated area. Mixing phenmedipham with clopyralid improved common ragweed control 11%, 21 days after application B (DAAB, 2-lf sugarbeet and 5-cm common ragweed) but provided similar control as clopyralid alone, 34 DAAB. Glufosinate mixed with clopyralid or glufosinate following clopyralid improved common ragweed control 22%, 28 DAAB (2-4 lf sugarbeet, 8 cm common ragweed) and extended common ragweed control 18% (40 DAAB) as compared to 2-times clopyralid application with glyphosate and Truvera™ sugarbeet. Clopyralid provides extremely effective common ragweed control in sugarbeet, however, its use has been confounded with signs of increasing common ragweed tolerance and extended emergence. Phenmedipham and glufosinate are herbicides potentially improving common ragweed control in sugarbeet.
Phosphorus – Testing our adequacy thresholds.
OLSEN, DAVEY*, ARIANNA HEGE and CRAIG FINDLAY, Amalgamated Sugar-SBR Research, 911 W 8th Street, Burley, ID 83318.
Abstract View Article
For healthy and productive sugarbeets, Amalgamated Sugar recommends soil test phosphorus (P) of at least 20-25 ppm-P in a 0–12-inch soil sample prior to planting. Although this critical threshold range has served us well for many years, we are often questioned by growers about why our P thresholds are so much lower than many of the commercial crop advisor companies that also service our industry. It is not uncommon for these advisors to recommend 100 lb or more P2O5 than we would for the same field. This discrepancy has led to some Amalgamated consultants and growers having a lack of confidence in our P recommendations. To address this, we established a phosphorus research trial over two years in 2021 and 2022. This micro-plot study evaluated sugarbeet productivity across a range of soil P levels from 5 ppm-P to 65 ppm-P. In addition, we also tested the ability of P-deficient beets to recover yield when amended with P2O5 at 2 or 4-leaf stage. Results showed that our current threshold range (20-25ppm-P) is adequate and that further increases in P level do not result in any additional yield or sugar.
Ethofumesate controls amaranthus spp. preemergence in sugarbeet.
MILLER, JULIANNA I.*1, THOMAS J. PETERS2, ADAM D. ABERLE2 and DAVID C. METTLER3, 1North Dakota State University, Dept. 7670, PO Box 6050, Fargo, ND 58108-6050, 2North Dakota State University and the University of Minnesota, NDSU, Dept. 7670, PO Box 6050, Fargo, ND 58108-6050, 3Southern Minnesota Beet Sugar Cooperative, 83550 County Road 21, Renville, MN 56284.
Abstract View Article
Ethofumesate use in sugarbeet in Minnesota and North Dakota has increased with the advent of glyphosate resistant weeds, especially glyphosate resistant waterhemp [Amaranthus tuberculatus (Moq.) J. D. Sauer]. Hectares treated with ethofumesate soil applied or postemergence increased from 28,045 to 163,047 or 480% between 2020 and 2023 at American Crystal Sugar Cooperative. Similar changes in grower practices have occurred at Southern Minnesota Beet Sugar Cooperative and Minn-Dak Farmers Cooperative. Multiple ethofumesate brands are used by producers including Nortron®, Maxtron 4SC®, Ethotron®, and Ethofumesate 4SC®. Three experiments were conducted at multiple locations in 2024 to evaluate: a) fall vs. spring ethofumesate application; b) waterhemp control with different brands; and c) incorporation (PPI) versus preemergent (PRE) ethofumesate application across rates and resultant length of redroot pigweed (Amaranthus retroflexus L.) control. Experiments were conducted near Beaver Creek, MN, Horace, ND and Moorhead, MN in 2024. Herbicides were applied using a bicycle wheel plot sprayer with a shielded boom to reduce particle drift and calibrated to deliver 159 L ha−1 through 8002XR nozzles (XR TeeJet® Flat Fan Spray Tips, TeeJet® Technologies, Glendale Heights, IL) spaced 51 cm apart and pressurized with CO2 at 207 kPa. Evaluations were a visible assessment of control by comparing the treated area in the plot to the bordering non-treated area. Waterhemp control following fall or spring ethofumesate application was the same 37 DAT (days after treatment) but control was better following at planting application, 47 DAT. Waterhemp control was the same across brands at Moorhead but Nortron, Maxtron and Ethotron controlled waterhemp better at Beaver Creek than Ethofumesate 4SC. Ethofumesate application PRE provided significantly better redroot pigweed control than PPI application. We attribute this to rainfall; site received 3 cm rainfall the evening of application. Ethofumesate PPI or PRE controlled pigweed better at 3.4-4.2 kg ha-1 than ethofumesate at 2.2 kg ha-1 24-44 days after application.
A comparison of different fertility programs.
OLSEN, DAVEY*, ARIANNA HEGE and CRAIG FINDLAY, Amalgamated Sugar-SBR Research, 911 W 8th Street, Burley, ID 83318.
Abstract View Article
Amalgamated Sugar provides fertility recommendations to growers with the goal of maximizing beet yield and sugar %, while minimizing beet juice impurities. These recommendations are based upon soil testing to a depth of 3 ft, and take into consideration historical performance of district, beet station, grower and field, as well as irrigation type, and prior crop. Although provided to growers as a free service, many growers choose instead to employ commercial agronomists and crop advisors to determine their fertility requirements for them. These companies determine their fertilizer requirements according to their own unique and propriety rationale. Their resulting fertilizer recommendations are usually very different from Amalgamated’s and often call for later season applications, more foliar micronutrient fertilizers, and higher levels of many crop nutrients than Amalgamated would recommend. This has resulted in much speculation from growers and crop advisors as to how each of these different fertility programs compare, and which is the most appropriate for sugarbeet production. We established a field trial to compare the fertility recommendations of three commercial advisory companies to those of Amalgamated Sugar. Results showed that Amalgamated’s fertility recommendations compare favorably to those of commercial advisors despite their much lower application rates.
Post-harvest application of atmospheric air cold plasma improves storage quality of sugar beet roots through alteration of microbiome and sugar metabolism.
MAJUMDAR, RAJTILAK*, CARL A. STRAUSBAUGH and PAIGE N. COX, USDA-ARS, Kimberly, ID 83341.
Abstract View Article
Post-harvest storage loss in sugar beets due to diseases and root respiration can lead to over 55% loss of sucrose. However, the application of an ecofriendly and convenient physical treatment, cold plasma (CP), can significantly improve post-harvest storage quality of sugar beet roots during prolong (>5-months) indoor storage. Using 4200 V plasma was created in a 48 cubic ft metal bin and sugar beet roots of two commercial cultivars were exposed for 15, 25, and 35 min at 3-week postharvest. Lower doses of CP reduced disease symptoms by 30-54% and higher dose (35 min) increased (16-25%) biomass retention. Sucrose retention increased (up to 18%) with CP, depending upon dose and genotype. Root microbiome analysis using 16S (bacteria) and ITS (fungi) sequencing, showed reduction of bacterial phyla such as Proteobacteria and Firmicutes, whereas Cyanobacteria increased with CP treatments. Altered beta diversity and significant reduction of potentially pathogenic (e.g. Proteobacteria) and biofilm producing (e.g. Actinobacteria) bacteria were observed in the CP treated samples. Cold plasma reduced fungal phylum, Basidiomycota, and the genera, Athelia sp. Overall, the results presented here demonstrate the potential of ecofriendly CP technology to improve post-harvest storage quality of sugar beets at commercial level in the future.
Finding the “sweet” spot: Nitrogen strategies for variable sugarbeet harvest timings.
FLEMING, SPENCER, ANDREW CHOMAS and KURT STEINKE*, Michigan State University, Department of Plant, Soil, and Microbial Sciences, Plant and Soil Sciences Building, 1066 Bogue Street, East Lansing, MI 48824.
Abstract View Article
Early sugarbeet (Beta vulgaris L.) harvest is a new challenge facing the Michigan sugarbeet industry. Due to sugar processing capacity limitations, earlier sugarbeet harvest is necessary to ensure beet processing is complete by mid- to late-March. Few data indicate how early or how late N can be applied and the subsequent effects on sugar quality. The objective of this field study was to evaluate the influence of harvest timing, planter-applied starter fertilizer, and in-season N fertilizer strategies on root yield, sugar quality, and in-season plant growth and development. Studies were arranged as a randomized complete block split-plot design with four replications. Main plots consisted of two harvest dates (early and conventional). Fertilizer strategy was the subplot factor and split into 1) two rates of starter N fertilizer (28-0-0) (0 and 60 lbs. N A) applied 2 inches to the side and 2 inches below the seed (2×2) at planting, and 2) seven sidedress (SD) in-season N fertilizer strategies. In-season N fertilizer strategies included: no N fertilizer application, 60 lbs. N band applied early June, 100 lbs. N A band applied early June, 60 lbs. N band applied early July, 100 lbs. N A band applied early July, 60 lbs. N band applied early August, and 100 lbs. N A band applied early August. In 2023, sugarbeet root yield and % sucrose were significantly impacted by harvest date with conventional harvest yielding +17.8 T/A and +4.3% greater sucrose compared to early harvest. For early harvest 2023, sidedress application timings had no impact on root yield where starter fertilizer was not applied. With starter fertilizer application during early harvest 2023, the low rate August SD and no SD treatments reduced root yield compared to remaining SD strategies. Starter decreased % sucrose -0.55 and -0.57% only for the June SD low and no SD treatments, respectively, during early harvest 2023. For conventional harvest 2023, an interaction between starter fertilizer and sidedress strategy occurred when starter was not applied. Due to starter fertilizer removing some yield variability, earlier N SD application resulted in greater root yield when starter was not applied. No interactions on % sucrose occurred for conventional harvest across fertilizer strategies. In 2024, early harvest root yield was greatest with June SD but no differences between low and high rates of June SD application. The June low SD rate yielded significantly greater than the low rates of July and August SD. Similarly, the June high SD rate yielded greater than the similar N SD rates applied in July and August. Results are not intended to highlight the downside or benefit from any single N management practice but rather to gather more data that will allow growers to remain flexible with N management strategies and allow growers to adjust in-season N practices based on variable harvest and climate scenarios.
Innovative extension using epoxy resin displays and time lapse photography.
FINDLAY, WALTER C.*, DAVEY OLSEN and ARIANNA HEGE, Amalgamated Sugar-SBR Research, 911 W 8th Street, Burley, ID 83318.
Abstract View Article
Agricultural extension is often more impactful if it relies on showing and demonstrating, as opposed to only telling. Using fresh plant materials in demonstrations and digging root pits for field days are two common extension resources currently used by ag researchers. Fresh plant material makes excellent extension content, particularly for showing leaf diseases. They can be passed around at grower meetings or field days and participants can see, touch, and feel the characteristics of a particular disease or weed. Unfortunately, however, they tend to degrade quickly, rely on time sensitive in-field collection, and are often season specific. Another common extension tool are root pits, which provide a great way to visualize the above and below ground portions of sugarbeets simultaneously. However, it is time consuming to dig and carefully wash the soil away to expose the roots. They are also immovable, non-permanent, and permanently disturb the soil footprint they are dug upon. To overcome the challenges of these extension materials, we developed methodologies for preserving fresh sugarbeet and weed plant material in epoxy resin blocks, and for using time lapse photography to show the above and below ground portions of a sugarbeet grown in custom-built plexi-glass boxes. Both of these methods have been very well received at grower meetings and field days and can be further customized and developed in the future.
A remote sensing approach to measuring in-season canopy cover percentage of sugarbeets.
FINDLAY, WALTER C.*, ARIANNA HEGE and DAVEY OLSEN, Amalgamated Sugar-SBR Research, 911 W 8th Street, Burley, ID 83318.
Abstract View Article
In agricultural research, optimizing and standardizing in-season data collection methodologies is critical for creating a workflow that produces accurate, reliable, and repeatable results. In trials where experimental treatments are expected to impact sugarbeet growth, canopy cover % provides valuable insights to help quantify treatment effects. Unfortunately, it is often time consuming and challenging to accurately capture canopy cover % in a truly representative way. Amalgamated Sugar agronomists have used a range of methods to measure canopy cover, including manually counting sugarbeet leaves and employing RGB imaging software such as the Canopeo App (http://www.canopeoapp.com). These methods have proven useful; however, they still have room for improvement. In 2024, we tried a remote sensing-based approach to address these concerns. We used unmanned aerial vehicle (UAV) technology paired with geospatial software to measure canopy cover percentages of field trials throughout the growing season. Images collected by the Mavic DJI 3M drone during autonomous flights were stitched using PIX4D Fields software, then uploaded into ArcGIS Pro where the Difference Vegetation Index (DVI) was used to calculate canopy cover percentage for the center two data rows of individual plots. This new method has greatly improved the accuracy of our canopy cover measurements, standardized our approach, and enabled us to confidently compare rates of canopy growth between different treatments in various locations and across multiple years. In 2025, we look to further expand on this work by using multispectral UAV technology to measure early season stand counts and a range of plant health metrics such as NDVI and NDRE.
Quick tests for sugar beet respiration.
EKELÖF, JOAKIM*1 and ANDRE WAUTERS2, 1Nordic Beet Research Foundation, Borgeby Slottsväg 11, SE – 237 91 Bjärred, 2IRBAB, Molenstraat 45, B – 3300 Tienen.
Abstract View Article
This study explores the development of a cost-effective and accelerated method for ranking sugar beet varieties based on their respiration rates, as a measure of storage stability. The COBRI group, in collaboration with NBR, utilized SenseCAP CO2 sensors to assess respiration in air-sealed containers, containing hand-harvested sugar beet samples. This new method, which provides results within 2 days, showed high correlation (r2 = 0,86) with advanced long term respiration measurements from IRBAB’s chambers, indicating its potential utility for quickly estimating storage-related sugar losses. The correlation between respiration and storage losses in this trial was high (r2 = 0,82). While the new method is only able to rank varieties according to respiration rate, this rate can be used to estimate storability of varieties. However, its weak correlation with total post-harvest sugar loss is due to its inability to capture losses from mold and rots during extended storage, particularly in machine-harvested beets in practice, which are significant sugar loss factors. Consequently, mechanical tests, such as penetration tests, may be more predictive of such deterioration-driven losses. This research provides a foundation for future iterations of rapid assessment tools to evaluate the storability of varieties, highlighting the need for developing quick tests for mold and rots to better estimate long-term storage losses.
Modelling the sugar beet storage environment.
ENGLISH, WILLIAM and JOAKIM EKELÖF*, Nordic Beet Research, Borgeby Slottsväg 11, 23791 Bjärred, Sweden.
Abstract View Article
Controlling and thus understanding the state of a sugarbeet postharvest storage system is important part of production. The rate of airflow is central in the thermodynamic processes that occur during storage. It determines the rate at which ambient air and the air inside the storage system exchange through advection, and the rates at which thermal energy and moisture transfer between the beets and the air. Unfortunately, the working environment has meant that direct measurement in a commercial setting has to date been beyond the available resources. Using Computational Fluid Dynamics (CFD) to model the airflow and thermodynamics in these systems has permitted insight to be gained. Using the open-source software programs R (physical model creation, and results analysis), OpenFOAM (CFD modelling), and ParaView (results visualization), a series of CFD models of stylized sugarbeet clamps were developed. The clamp was modeled as 9 m wide and 3 m high, and as either uncovered, or covered with the non-woven polypropylene fleece TopTex®. Experimental data for temperature in the clamp and ambient temperature and wind velocity was taken from experiments conducted by NBR, Sweden, in 2011/12. The porous medium approach was adopted, and Darcy-Forchheimer (D-F) parametrization applied. The D-F values for the clamp region were derived from previous research (Tabil et al, 2003), alternatively by applying a variant of the Ergun equation. D-F values for TopTex were supplied by SolMax (previously known as TenCate Industrial Fabrics). This poster will present results from the first version of the model, with a focus on the airflow dynamics. This project is part of a larger ongoing series looking to develop models of fluid- and thermodynamics in sugarbeet postharvest storage systems that will be used in research and in the information industry sends to growers.
Integrating multi-source remote sensing data and machine learning for large-scale sugarbeet yield forecasting in the Western High Plains region.
CABRERA, MARIA*, FELIU SERRA-BURRIEL, RAFAEL FORTES, ROCÍO GARCÍA, CARLOS FERRAZ, XAVIER SILVA, NICK MILHOUS, GIL SALA and PABLO MORAN, HEMAV Agritech Inc., 2655 N Le Jeune Rd, Suite 810, Miami, FL 33142.
Abstract View Article
Accurate yield forecasting is fundamental for agricultural management, yet existing models and procedures often struggle with scalability. The integration of artificial intelligence with remote sensing technologies presents an opportunity to improve prediction accuracy across large geographical areas. This study focused on developing and validating a predictive model for tonnage, sucrose content, and polarization in sugarbeet fields across the Yellowstone, North Platte, and South Platte River valleys. The model was trained using 1,379 field-seasons of historical data, combining multi-temporal satellite imagery and climate data with geo-referenced yield measurements. The data was processed through the LAYERS® PredTech infrastructure, employing machine learning algorithms to generate field-level predictions. The model achieved Mean Absolute Percentage Error (MAPE) below 5% for all three predicted variables (tonnage, sucrose, and polarization). These results demonstrate the potential for large-scale implementation of remote sensing-based yield forecasting in sugarbeet production. The study was validated through the 2024 season and will continue through the 2025 season.
Impact of defoliation timing.
BURT, EMMA L.*, MICHAEL S. METZGER, PAUL J. MOFFET and BRADLEY L. SCHMIDT, Minn-Dak Farmers Cooperative, 7525 Red River Rd, Wahpeton, ND 58075.
Abstract View Article
Defoliation timing was evaluated in Minnesota in 2021, 2022, and 2023. Sugarbeet was defoliated 7 days before, 5 days before, 3 days before, 1 day before, and on the same day as harvested. Sugarbeet quality decreased as the time between defoliation and harvest increased. Across the three-year run of the study, there was an average decrease in percent sugar of 0.2, which translated to an average loss of 65 pounds of recoverable sugar per acre just by defoliating 24 hours ahead of the harvester. There was a decrease in revenue of an average of $15 per acre per day for the first 5 days that the sugarbeets were defoliated ahead of the harvest date. This was based on a crop payment of $0.22 per pound of extractable sugar. A loss of $15 per acre per day defoliated ahead of the harvester is approximately $2,325 per day across an entire quarter.
Identification of genomic regions associated with root respiration rate during post-harvest cold storage.
BOSTAN, AHMET BARKAN*1, JOLENE ADDINGTON1, LISA PREISTER2, KAREN FUGATE2, NATHAN WYATT2, MELVIN BOLTON2, XUEHUI LI1 and CHENGGEN CHU*2, 1North Dakota State University, Department of Plant Sciences, Fargo, ND 58102, 2USDA-ARS, Edward T. Schafer Agricultural Research Center, Sugarbeet Research Unit, Fargo, ND 58102.
Abstract View Article
Sugarbeet (Beta vulgaris L. ssp. vulgaris Doell.) is primarily grown for sucrose production. Sugarbeet roots, which are the main part containing sucrose, are often stored in piles at outdoor under cold up to six months after harvesting. A significant sucrose loss during root storage occurs due to root respiration to provide energy for surviving as well as healing wounds from harvesting or making root files. Reducing root respiration rate during storage could help minimize sugar loss. In this study, sugarbeet lines along with some fodder and table beet genotypes were tested for root respiration rates after stored at 4 ˚ C for one to three months, and a genome-wide association study (GWAS) will be conducted to identify genomic regions associated with respiration rates in different genotypes and the changes of the respiration rate during cold storage. DNA markers will be developed from the associated genomic regions for utilizing genotypes with lower respiration rates for germplasm enhancement. This study will not only identify genotypes for developing germplasm with lower storage respiration rate, but also the understanding genetic control of respiration rate, which will be critical for efficiently breeding new cultivars with lower respiration rate during cold storage.
Sugarbeet yield and recoverable sucrose response to intensive nutrient management.
BEARSS, LANE S., ANDREW CHOMAS and KURT STEINKE*, Michigan State University, Department of Plant, Soil, and Microbial Sciences, Plant and Soil Sciences Building, 1066 Bogue Street, East Lansing, MI 48824.
Abstract View Article
Michigan sugarbeet (Beta vulgaris L.) nutrient management recommendations include 157-179 kg N ha with an initial 45 kg N ha applied at planting to promote canopy closure. While individually added inputs associated with yield gaps were previously investigated, synergistic influences when combined with a standard N program (SN) within an integrated management perspective have not been explored. This study investigated sugarbeet root yield and recoverable sucrose response to different fertilizer strategies along a stepwise increase in management intensity. In 2022, SN treatment averaged 90.1 Mg ha, 148.4 kg Mg-1and 13,327.9 kg ha-1in root yield, recoverable sugar per ton and recoverable sugar per hectare, respectively. The addition of in-furrow P negatively impacted root yield and recoverable sugar by -15.5 Mg ha and – 2,325.7 kg ha, respectively. In 2023, pre-plant broadcast lime, in-furrow P, and intensive management (combining all individual inputs) increased root yield by 13.7, 11.9, and 13.2 Mg ha, respectively. The intensive management and pre-plant broadcast lime increased recoverable sugar per Mg by +7.1 and +8.4 kg Mg, respectively, while also improving recoverable sugar per hectare by +2,329.8 and +2,278.0 kg ha, respectively. In-furrow P increased sugar per hectare by 2,186.3 kg ha. The inconsistent root yield and recoverable sucrose response to marketed inputs accentuate the importance of pre-plant soil analysis, in-season weather monitoring, and the use of disease models for developing a climate-smart agricultural system.
Weeds, catch crops and flower strips as potential risk for virus yellows transmission in sugar beet.
BAENSCH, SVENJA*1, TJARK P. PAULSEN2, NILS KLINGEMANN1 and WITOON PURAHONG1, 1KWS SAAT SE & CO KGaA, Grimsehlstraße 31, 37574 Einbeck, Germany, 2Osnabrück University of Applied Sciences, Albrechtstraße 30, 49076 Osnabrück, Germany.
Abstract View Article
Virus yellows is a disease affecting sugar beet that can lead to significant economic losses in Europe. This disease is linked to a complex of three different viruses: Beet yellows virus (BYV), Beet mild yellowing virus (BMYV), and Beet chlorosis virus (BChV). The primary vector for these viruses is the peach-potato aphid, Myzus persicae, which has many host plant species besides sugar beet. However, there is limited information on alternative host plants from agroecosystems for these viruses. This knowledge is crucial because weeds and other plant species that host one or more of the viruses and can overwinter may serve as a virus source in the following year. We investigated whether plant species growing in or near sugar beet fields in Europe can act as host plants for one or more of these viruses. These plants can grow as weeds within sugar beet fields, in flower strips adjacent to fields, or as catch crops preceding the main crop. We selected 22 common plant species and inoculated them with M. persicae carrying a mixture of BYV, BMYV, and BChV. We inoculated 8 plants per species, pooled two plants respectively (resulting in 4 samples per plant species), and checked for the presence and identity of viruses using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Out of the 22 plant species, four were infected with BYV, 17 with BMYV, and three with BChV. Depending on the plant species, one to four out of four samples were infected with one or two viruses. For instance, in Cirsium arvense, an economically significant weed species, we identified BYV in three samples and BMYV in one sample. In Phacelia tanacetifolia, a common species in flower strips and catch crops, we detected BYV in three samples and BMYV in four samples. Among the plant species identified as virus hosts, several can overwinter in mild conditions, such as C. arvense and P. tanacetifolia. Therefore, they can serve as a virus source in the following year. These findings should be considered not only for weed management but also when selecting flower mixtures for flower strips and catch crops as part of agri-environmental schemes in Europe. To clarify the risk of transmission from virus host plants identified in this study to sugar beet, further research is needed to test the re-infection from infested plants back to sugar beet.
Amaranthus palmeri survey, confirmation, and resistance testing in Idaho and Oregon sugar beet growing regions.
ALDER, CLARKE G.*1, ALBERT ADJESIWOR2, JOEL FELIX3, SKYLEN HAWKES4 and EMILY CORGATELLI1, 1Amalgamated Sugar Company, Sugar Beet Research, Nyssa, OR 97913, 2University of Idaho Kimberly Research and Extension Center, Kimberly, ID 83341, 3Oregon State University, Malheur Experiment Station, Ontario, OR 97914, 4Amalgamated Sugar Company, Nampa District Ag Staff, Nampa, ID 83687.
Abstract View Article
Amaranthus palmeri has been sighted on only a couple of occasions in southwestern Idaho since 2021. These were isolated incidents and plants were either pulled or otherwise destroyed. In July 2023, staff of Amalgamated Sugar discovered a large patch bordering a potato field in southwest Idaho. Within days of this discovery, Agricultural Staff at Amalgamated Sugar, in collaboration with University of Idaho and Oregon State University staff conducted a full-scale survey of southern Idaho and eastern Oregon to help identify additional populations of A. palmeri. As of the end of 2023, 71 populations of A. palmeri had been identified and 23 sampled for genetic confirmation and resistance testing. Additionally, 4 populations of Amaranthus tuberculatus were discovered in sugar beet fields in southern Idaho. These populations were also sampled and analyzed. As of the end of 2024, 107 populations of A. palmeri and 4 populations of A. tuberculatus have been identified in southern Idaho and eastern Oregon. Nearly all A. palmeri populations sampled tested positive for glyphosate resistance, and some also containing ALS resistance. All populations of A. tuberculatus were positive for glyphosate resistance and only one containing additional ALS resistance. Additional resistance screening is ongoing at University of Idaho to determine additional HRAC group resistance in A. palmeri populations in Idaho. This discovery represents a distinct challenge for sugar beet growers in southern Idaho and eastern Oregon.
Impact of herbicide carryover and drift on sugar beet yield and a decision-making guide for growers experiencing impact from non-target herbicide effects.
ALDER, CLARKE G.*1, ALBERT ADJESIWOR2, EMILY CORGATELLI1 and TYLER MORRIS1, 1Amalgamated Sugar Company, Sugar Beet Research, Nyssa, OR 97913, 2University of Idaho Kimberly Research and Extension Center, Kimberly, ID 83341.
Abstract View Article
Each year crop consultants and agronomists are contacted by growers needing assistance with issues regarding herbicide applications both past and present. Despite pesticide label instructions, consultant recommendations, and advanced spray technologies, issues involving both carryover and drift of herbicides into non-target crops such as sugar beets continue to be a problem each season. On occasion, a grower may experience a scenario where a speedy decision needs to be made whether to keep a sugar beet crop for the duration of the season or replace it with an alternative crop. Trials were initiated at two locations in 2022, 2023, and 2024 evaluating the impacts of both carryover and drift of several herbicides representing HRAC groups common within crop rotations in southern Idaho and eastern Oregon. The object of the studies is to develop a guide aiding growers in the decision of whether to keep an impacted sugar beet crop for the duration of the season. Impacts from the herbicides have been largely yield-driven with minimal impact on sugar content. Storability, processability, and even the ability to harvest properly may be a problem depending on the herbicide group affecting the sugar beet crop and is being assessed for the 2024 trials.
Evaluation of root firmness and cell wall components in sugarbeet lines for reducing sugar loss during post-harvest piling storage.
ADDINGTON, JOLENE*1, AHMET BARKAN BOSTAN1, LISA PREISTER2, KAREN FUGATE2, NATHAN WYATT2, MELVIN BOLTON2, XUEHUI LI1 and CHENGGEN CHU*2, 1North Dakota State University, Department of Plant Sciences, Fargo, ND 58102, 2USDA-ARS, Edward T. Schafer Agricultural Research Center, Sugarbeet Research Unit, Fargo, ND 58102.
Abstract View Article
Roots of sugarbeet (Beta vulgaris L. ssp. vulgaris Doell.) are easily injured during harvesting or making piles for storage prior to processing in sugar factory, which not only increases the risk of roots being infected by pathogens to cause root rot and affecting sucrose extraction, but also enhances respiration of root tissue to provide energy for wound healing and causing significant sugar loss. Increase root firmness to reduce root injuries during harvesting and post-harvest piling storage will provide a solution to reduce post-harvest sugar loss. Root tissue firmness is determined by strength of tissue cells and cell wall structure could be a key determinant of tissue texture. Analyzing content of pectin types (mainly water-soluble pectin, sodium-carbonate-soluble pectin and chelator-soluble pectin) in cell wall will provide critical information of increasing root firmness via breeding selection focuses on cell wall components. In this study, we will evaluate root tissue firmness and cell wall components in 360 sugarbeet genotypes to find out the relationship between root firmness and cell wall pectin compositions before and after roots were cold stored for three months. A genome-wide association study (GWAS) will be conducted to identify genomic regions associated with root firmness and cell wall pectin components to develop markers linked to genes that increase root hardness. This research will identify genetic sources and develop markers for efficiently creating new germplasm with enhanced root hardness towards reducing sugar loss during harvesting and post-harvest piling storage.
Response of sugar beet to group 15 herbicides applied pre or delayed preemergence.
ADJESIWOR, ALBERT1, CLARKE G. ALDER*2 and JOEL FELIX3, 1University of Idaho Kimberly Research and Extension Center, Kimberly, ID 83341, 2Amalgamated Sugar Company, Sugar Beet Research, Nyssa, OR 97913, 3Oregon State University, Malheur Experiment Station, Ontario, OR 97914.
Abstract View Article
During crop years 2023 and 2024 staff at Amalgamated Sugar, University of Idaho, and Oregon State University fielded several calls regarding Palmer amaranth and waterhemp in sugar beet, right-of-way, and several other crops. About 70% of the Palmer samples submitted during 2023 and 100% of the waterhemp samples submitted were confirmed glyphosate resistant. Group 15 herbicides such as acetochlor, dimethenamid-P, and S-metolachlor are very effective or Palmer amaranth and waterhemp control. However, these herbicides are only labeled to be applied when sugar beets reach the 2-leaf stage. Trials were initiated in three locations in Oregon and Idaho during 2024 to evaluate the safety of Warrant®, Dual Magnum®, and Outlook® applied preemergence on sugar beet for early season weed control. An additional factor, a seed treatment, fluxofenim, used as a safener on grain and forage sorghum seed against S-metolachlor injury, was also evaluated for efficacy on sugar beet seed.
Physiology, Genetics, and Plant Pests - Oral
Identification of genomic regions associated with Beet curly top virus resistance in the sugar beet double haploid line, KDH4-9.
MAJUMDAR, RAJTILAK*1, EVAN M. LONG1, CARL A. STRAUSBAUGH1 and IMAD A. EUJAYL1, 1USDA-ARS Northwest Irrigation & Soils Research Lab, 3793 North 3600 East, Kimberly, ID 83341, USA.
Abstract View Article
Beet curly top virus (BCTV) reduces sugar beet yield and sucrose production in semi-arid sugar beet growing regions. Control of BCTV in commercial sugar beet cultivars is based on low to moderate genetic resistance and the use of neonicotinoid insecticide seed treatments which may be restricted in the future due to environmental concerns. The Kimberly ARS program has developed sugar beet double haploid breeding lines such as KDH4-9 (R) exhibiting strong resistance to BCTV. When KDH4-9 was crossed with the highly susceptible (S) line, KDH19-17, the BCTV resistance trait segregated into three distinct resistance groups in the F2 population. We used graded-pool sequencing to rapidly map QTLs through whole-genome sequencing and bulked-segregant analysis. Graded-pool sequencing of individuals from the R and S groups (segregating population) and parents when combined with transcriptome analysis, revealed resistance associated QTLs primarily localized in chromosome 3 and a few minor QTLs in chromosomes 1, and 2. Resistance related genes identified were Bevul.3G031800 (RNA transport), Bevul.1G037400 (DNA-directed RNA polymerase III), Bevul.2G154500 (amino acid transmembrane transport). The putative genomic markers associated with BCTV resistance in KDH4-9 will be valuable for future trait introgression into commercial cultivars.
Incorporating disease onset indicators for Cercospora leaf spot risk modeling and fungicide application management.
WYATT, NATHAN A.*1, VIVIANA RIVERA2, GARY A. SECOR2, and MELVIN D. BOLTON1, 1United States Department of Agriculture, Sugarbeet Research Unit, Fargo, North Dakota, USA, 2North Dakota State University, Department of Plant Pathology, Fargo, North Dakota, USA.
Abstract View Article
Cercospora leaf spot (CLS), caused by the fungal pathogen Cercospora beticola, is the most economically important disease of sugar beet. If left unmanaged, CLS can significantly reduce crop yields and sugar content. Timely fungicide applications are critical management tools used by growers to combat CLS with the timing of applications informed by disease forecasting models. Currently deployed CLS forecasting models rely heavily on relative humidity and temperature as indicators of symptom onset but neglect indicators of infection initiation. Recently, field studies involving spore trapping and CLS infection progression monitoring have revealed high spore abundance early in the growing season leading to the initiation of asymptomatic infection prior to symptom onset. As fungicide applications are not curative, synchronizing fungicide applications with asymptomatic infection onset may present an opportunity to better manage CLS and indicators of infection onset need to be incorporated into CLS risk models. Lab and greenhouse studies have shown that C. beticola spores can germinate at temperatures as low as 10℃ if moisture conditions are favorable. Here we used weather sensors and correlated environmental conditions that correspond to favorable spore germination conditions and propose these parameters be incorporated into existing CLS risk models. This two-part model system has potential to increase the utility of CLS risk models to better target fungicide applications to be maximally protectant against CLS disease onset.
Whole genome sequencing reveals population structure of Cercospora beticola to resistant sugar beet cultivars in Germany.
YANG, YIXUAN1, NATHAN WYATT2, MARK VARRELMANN*1, SEBASTIAN LIEBE1, and MELVIN D. BOLTON2, 1Institute of Sugar Beet Research, Dpt. Phytopathology and Dpt. Coordination, Holtenser Landstraße 77, D-37079 Göttingen, Germany, 2USDA-ARS, Sugarbeet Research Unit, Fargo, ND.
Abstract View Article
Cercospora leaf spot (CLS), caused by the fungal pathogen Cercospora beticola, is the most destructive sugar beet foliar disease. Management of CLS relies heavily on fungicide applications, however the emergence of fungicide-resistant populations emphasizes the importance of developing resistant cultivars. Understanding the interactions between cultivar resistance and C. beticola is essential for sustainable CLS management. To gain better insight into the structure of C. beticola populations collected from cultivars bearing distinct resistance properties, field trials were conducted with four sugar beet cultivars with different resistance properties in four geographical locations in Germany in 2022 and 2023. Experimental plots were inoculated with CLS-infested leaf material in 2022. Leaf material from each variety was harvested separately at the end of the season as inoculum for 2023. A population of 25 C. beticola isolates was obtained from each cultivar at each location, resulting in a total number of 800 isolates in both years. Isolates were subjected to whole genome sequencing and variant discovery to perform population genomic analysis. Principal component analysis showed little variation among populations obtained from different locations or cultivars, although location-specific lineages were identified in both years from one of the trial locations. Population genomic scans revealed eight genomic regions associated with certain host genotypes. Further experiments will focus on validating the functions of genes underlying these associated regions.
Breeding for genetic tolerances against leaf diseases based on a unique, combined gene pool.
TSCHOEP, HENDRIK*1, JUAN VEGAS1, BRITT-LOUISE LENNEFORS1, and TYLER RING2, 1United Beet Seeds, Industriepark 15, 3300 Tienen, Belgium, 2United Beet Seeds, 1020 Sugar Mill Road, Longmont, CO 80501, USA.
Abstract View Article
Cercospora beticola is known as the most severe leaf disease in sugar beets, but during recent years Alternaria leaf spot caused by Alternaria spp, in several growing regions has triggered additional production challenges. Resistance to fungicides is identified among both C. beticola and Alternaria spp isolates and therefore sugar beet varieties with broad tolerances are becoming increasingly important. The breeding company United Beet Seed (UBS) has unique access to diverse gene pools from two companies, and combined in a complementary way, different sources of tolerances which provide growers with high-yielding, sustainable sugar beet varieties. Different genetic loci were stacked to achieve a high-level of tolerance against a wide range of isolates, leading to durable tolerance for future sugar beet varieties.
Characterization of Cercospora beticola populations to support integrated Cercospora leaf spot management approaches in Michigan.
WILLBUR, JAIME F.*1, ALEXANDRA P. HERNANDEZ1, SARAH RUTH1, CHRIS BLOOMINGDALE1, and LINDA E. HANSON2, 1Michigan State University, 612 Wilson Road, 35, Plant Biology Lab, East Lansing, MI 48824, 2USDA-ARS, 612 Wilson Road, 37, Plant Biology Lab, East Lansing, MI 48824.
Abstract View Article
Sugarbeets produce over half of domestic sugar, and Michigan is the fourth highest U.S. producer. Cercospora beticola causes one of the most impactful foliar diseases in sugarbeet growing regions, Cercospora leaf spot (CLS). Management of CLS relies heavily on timely and repeated fungicide applications, and fungicide resistance has been observed for several fungicide classes in C. beticola. In 2021 and 2022, in vitro fungicide sensitivity was tested for five demethylation inhibitors (DMI; difenoconazole, fenbuconazole, mefentrifluconazole, prothioconazole, tetraconazole), a quinone outside inhibitor (QoI; pyraclostrobin), a methyl benzimidazole carbamate (MBC; thiophanate-methyl), and triphenyltin hydroxide. Mutations associated with resistance to QoI, MBC, and DMI fungicides also were evaluated using restriction fragment length polymorphisms (PCR-RFLP). While the E198A mutation was significantly associated with C. beticola responses to thiophanate-methyl (P < 0.0001), G143A and Glu169 were not as strongly associated with pyraclostrobin or DMI (prothioconazole and tetraconazole) responses, respectively. Overall, in vitro insensitivity was observed for all tested active ingredients, however, elevated frequencies were observed for thiophanate-methyl (72-86%), tetraconazole (28-56%), prothioconazole (78-92%), and pyraclostrobin (9-33%). Despite these observations, application programs that rotate multiple fungicide groups and use tank-mix partners continue to offer significant CLS control in field studies (P < 0.05). To further diversify and support integrated CLS management, ongoing studies of cultural strategies to reduce C. beticola inoculum survival also are being conducted.
Seasonal spore production, germination and fungicide resistance shifts of Cercospora beticola in commercial sugar beet fields.
SECOR, GARY*1, VIVIANA RIVERA1 and NATHAN WYATT2, 1North Dakota State University, NDSU Dept. 7660, PO Box 6050, Fargo, ND 58108, 2USDA ARS, Schafer Agricultural Research Center, 1616 Albrecht Blvd., Fargo, ND 58102.
Abstract View Article
Cercospora leaf spot (CLS) caused by Cercospora beticola (Cb) is the most important foliar sugarbeet disease. Management includes cultural practices, resistant varieties and timely fungicide applications. Disease prediction models monitor conditions for disease spread and fungicide application after CLS is present, but do not include spore production and germination that may be important for predicting protective fungicide application prior to disease appearance. Laboratory experiments indicate spore germination begins in two hours at 10°C, is higher in free water and increases with time and temperature. At low temperatures, spores from fungicide resistant isolates tend to have lower germination rates compared to fungicide sensitive isolates. Cb spores were detected in commercial sugar beet fields annually from 2021 – 2024 before emergence using Spornado spore traps and PCR testing. Seasonal testing showed changes in fungicide resistance to pyraclostrobin. We conclude that forecasting models for CLS should include spore detection and early wetness conditions and adjusted to recommend fungicide applications earlier in the growing season before infection by Cb.
Extreme-phenotype GWAS identified genomic variation associated with resistance to Aphanomyces root rot in sugar beet.
RUDE, SAMANTHA C.*1, OLIVIA E. TODD2, KEVIN M. DORN2, CORY D. HIRSCH1, and ASHOK K. CHANDA1,3, 1University of Minnesota, Dept. of Plant Pathology, 1991 Upper Buford Circle, St. Paul, MN, 55108, 2USDA-ARS, Center for Agricultural Resources Research, 2150 Center Ave., Bldg. D, Fort Collins, CO, 80526, 3University of Minnesota Northwest Research & Outreach Center, 2900 University Avenue, Crookston, MN, 56716.
Abstract View Article
Aphanomyces root rot (ARR) can result in significant damage to newly emerged sugar beet seedlings. ARR is primarily managed by incorporation of factory waste-lime, use of fungicide seed treatments and use of moderately resistant varieties. Limited breeding efforts have focused on improving adult plant resistance although the mechanisms of resistance are unclear. Ninety-six USDA-ARS pre-breeding lines were screened for ARR resistance using a zoospore inoculation method and scored using a standardized 0-3 ARR rating scale. Individual plants within an accession were selected and pooled into equally sized (n=106) highly resistant or highly susceptible pools. Genomic sequencing was conducted on the pools for an extreme phenotype genome-wide association study (XP-GWAS) to identify genomic variants associated with ARR resistance. A total of 5,286,985 biallelic SNPs were identified and 29 of those were found to be statistically significant. These highly significant variants between pools were used to identify novel regions of the genome associated with ARR resistance.
Virus yellows disease in sugar beet – a resistance mechanism for the future.
ROLLWAGE, LUKAS*1, HILDE VAN HOUTTE2, ROXANA HOSSAIN1, NIELS WYNANT2, GLENDA WILLEMS2, and MARK VARRELMANN1, 1Institute of Sugar Beet Research, Holtenser Landstraße 77, D-37079 Goettingen, Germany, 2SESVanderHave NV., Industriepark 15, BE-3300 Tienen, Belgium.
Abstract View Article
The virus yellows disease (VY) in sugar beet is caused by several aphid-transmissible viruses. Namely, beet yellows virus (BYV, Closterovirus), beet mild yellowing virus (BMYV) and beet chlorosis virus (BChV) (both Polerovirus). Due to a similar transmission mechanism and synergistic effects, beet mosaic virus (BtMV, Potyvirus) is often considered YV associated. Apart from BYV, all aforementioned viruses carry a protein called viral protein genome linked (VPg) that is covalently bound to their genomes 5’ end. The VPg is critical for translation initiation and thus viral protein biosynthesis within its host. Previous studies show that potyviral VPgs often interact with plant susceptibility factors the eukaryotic translation initiation factors (eIFs). Furthermore, initial studies in Arabidopsis thaliana support that this concept might be used to control paleoviruses as well. If the VPg-eIF interaction is disturbed e.g., by mutations, the virus is unable to replicate within the plant. Due to its heredity, this mechanism is called recessive resistance. We used molecular biological methods such as yeast two hybrid and bimolecular fluorescence complementation to identify different sugar beet eIF isoforms as protein-interaction partners for the respective VY-VPgs. Subsequently, T0 knockout sugar beets of the corresponding candidate genes were generated by genome editing and subjected to a resistance test. Knockout of Bv‑eIF(iso)4E resulted in resistance to BChV, which significantly reduced the virus titer as well as infection rates compared to unedited plants. By this making Bv-eIF(iso)4E the first known resistance mechanism to a member of VY in sugar beet and the first recessive resistance to poleroviruses in a food crop. Furthermore, this work shows for the first time the suitability of genome editing in sugar beet by focusing on an agronomical important trait. Meanwhile, resistance to BMYV and BtMV could not be obtained. An explanation for this could be simultaneously detected interactions of BMYV-VPg with Bv-eIF(iso)4E and Bv-eIF4E, therefore potentially requiring a double knockout of the respective eIF4Es, which could not be obtained due to lethality of the mutation. Still, after showing that recessive resistance to poleroviruses can be implemented in the sugar beet host system, this knowledge can be used in the future for breeding purposes to identify natural resistance genes or further editing targets. Rollwage, L; van Houtte, H; Hossain, R; Wynant, N; Willems, G. and Varrelmann, M. (2024) Recessive resistance against beet chlorosis virus is conferred by the eukaryotic translation initiation factor (iso)4E in Beta vulgaris. Plant biotechnology journal, 22, 2129–2141.
Battling Cercospora Leaf Spot in an ever-changing world.
REKOSKE, M. M.*1, H. KEUNECKE2, H. EBMEYER2, and J.C. LEIN2, 1KWS Seeds, LLC. 1325 Valley View Road, Shakopee, MN 55379, 2KWS SAAT SE & Co. KGaA, Grimsehlstrasse 31, 37574 Einbeck, Germany.
Abstract View Article
Cercospora leaf spot (CLS) is one of the most detrimental leaf diseases of sugarbeet and of high economic importance for many sugarbeet production regions worldwide. In 2024, the Eastern commercial growing areas of North America experienced an extraordinary growing season that was characterized by exceptionally high disease pressure of CLS compared to average years. A main driver was favorable weather for CLS development, placing extreme demands on the available CLS management tools, from the use of fungicides and crop rotation to varieties. Even with CR+® hybrids, which have demonstrated high levels of CLS protection over the past three years, CLS control was often challenging in 2024. Contributing factors are currently under analysis including a potential adaptation of the Cercospora population in different growing regions. The composition of the Cercospora population is addressed by KWS large scale monitoring and carried out with isolate specific markers. Continual improvements in the CLS tolerance through breeding and the optimization of other available measures are needed to control CLS effectively.
Identification and characterization of beet necrotic yellow vein virus-derived small RNAs from sugarbeet by high-throughput sequencing.
RAMACHANDRAN, VANITHARANI*1, CHINNARAJA CHINNADURAI2, ALYSSA FLOBINUS2, NATHAN WYATT1, JOHN WEILAND1, and MELVIN BOLTON1, 1USDA-ARS, Edward T. Schafer Agricultural Research Center, 1616 Albrecht Blvd N, Fargo, ND 58102, 2North Dakota State University, Fargo, ND 58102.
Abstract View Article
In plants, virus infection triggers the accumulation of virus-derived small RNAs due to an endogenous host defense mechanism called RNA interference (RNAi). Beet necrotic yellow vein virus (BNYVV) is the causal agent of the economically significant rhizomania disease of sugarbeet. In this study, using high-throughput small RNA sequencing, we analyzed the frequency and distribution of virus-derived small RNAs of BNYVV to explore RNAi-based antiviral strategy for rhizomania disease management. The roots of sugarbeet seedlings were infected with sap obtained from inoculating RNA transcripts of infectious clones of RNA1, RNA2, RNA3, and RNA4 of BNYVV. At three different time points, virus-infected root samples were collected, and high-throughput small RNA sequencing was carried out for each time point along with mock-inoculated control. Mapping of virus-derived small RNAs in both sense- and antisense-orientations to BNYVV genome revealed the frequency and distribution patterns of small RNA in the root tissue of sugarbeet. This study details small RNA hotspots on various RNA segments of the BNYVV genome, which can aid in developing RNAi-based antiviral strategies for managing rhizomania disease.
Identification and characterization of Cercospora beticola effector candidates associated with adaptation to the sugarbeet resistance gene BvCr4.
NATWICK, MARI B.*1,2, NATHAN A. WYATT1, GARY A. SECOR2, and MELVIN D. BOLTON1, 1United States Department of Agriculture, Northern Crop Science Laboratory, Fargo, North Dakota, USA, 2North Dakota State University, Department of Plant Pathology, Fargo, North Dakota, USA.
Abstract View Article
Cercospora leaf spot (CLS), caused by the fungal pathogen Cercospora beticola, is the most economically important disease of sugarbeet. If left unmanaged, CLS can significantly reduce crop yields and sugar content. Recently, growers have adopted the use of commercial varieties utilizing the resistance gene BvCr4 (CR+) to help manage CLS. Unfortunately, populations of C. beticola that are capable of overcoming BvCr4 have been identified worldwide. Although a genome wide association study (GWAS) conducted on European populations of C. beticola identified a single gene (AvrCr4) associated with resistance mediated by BvCR4, there was no subsequent functional validation of this gene. Moreover, it is not clear if American C. beticola populations also utilize this gene to overcome the BvCr4 resistance mechanism. To gain an understanding of how American C. beticola strains have adapted to BvCr4, we conducted a population genomic analysis with populations collected from sugarbeet plants throughout American growing regions with and without BvCr4. Through this analysis, we identified 14 fungal genes putatively associated with the ability to overcome BvCr4. We have developed C. beticola mutants lacking these candidate genes to functionally validate them. Characterization of these mutants will be presented. This research aims to elucidate the interaction between C. beticola and the CLS-resistance gene, BvCR4, in order to broaden understanding of the BvCr4 resistance phenotype and predict durability of similar resistance genes. In addition, this knowledge will allow for the design of molecular-based assays to identify BvCr4-resistant strains.
Exploring the origin and phylogeny of Cercospora beticola resistance gene BvCR4 in geographically diverse populations of wild sea beet (Beta vulgaris subsp. maritima).
MARTINEZ, ANA L.*1,2, GARY SECOR2, CHENGGEN CHU1, MELVIN D. BOLTON1 and NATHAN A. WYATT1, 1USDA-ARS-PA, Edward T. Schafer Agri Research Center, 1616 Albrecht Blvd. North, Sugarbeet Research, Fargo, ND 58102, 2North Dakota State University, 1340 Administration Ave., Plant Pathology, Fargo, ND 58102.
Abstract View Article
Cercospora beticola is the causal agent of Cercospora leaf spot (CLS), a devastating disease of sugarbeet worldwide. The gene BvCR4 (CR+) that encodes a plant pattern recognition receptor conferring resistance to CLS was recently introgressed from sea beet into cultivated sugarbeet. To investigate the origin and evolution of this gene, 619 wild and cultivated beet accessions from geographically diverse backgrounds were retrieved from the Germplasm Resource Information Network that contained historic CLS disease phenotypic data. Accessions were screened for BvCR4 homologs by PCR and the obtained amplicons were sequenced. Phylogenetic relationship between homologs was determined on both coding and amino acid (AA) sequences. A total of 27 accessions produced amplicons, 19 encoded proteins with 79 to 92 % AA sequence identity with BvCR4, while eight were predicted to be non-functional proteins due to premature stop codons. Phylogenetic analyses showed that Italy or Greece is likely the geographic origin of the resistant trait. BvCR4 homolog sequence variation exhibits a high number of AA differences that may impact binding affinity with the corresponding ligand. However, a 25 AA segment was exclusively present in resistant accessions, potentially critical for pathogen pattern recognition and host resistance. Functional variations of BvCR4 in sea beet populations will be valuable for CLS resistance breeding and provide a better understanding of BvCR4 resistance against Cb to anticipate how the pathogen may subvert resistance.
Identification of genomic regions associated with Beet curly top virus resistance in the sugar beet double haploid line, KDH4-9.
MAJUMDAR, RAJTILAK*1, EVAN M. LONG1, CARL A. STRAUSBAUGH1 and IMAD A. EUJAYL1, 1USDA-ARS Northwest Irrigation & Soils Research Lab, 3793 North 3600 East, Kimberly, ID 83341, USA.
Abstract View Article
Beet curly top virus (BCTV) reduces sugar beet yield and sucrose production in semi-arid sugar beet growing regions. Control of BCTV in commercial sugar beet cultivars is based on low to moderate genetic resistance and the use of neonicotinoid insecticide seed treatments which may be restricted in the future due to environmental concerns. The Kimberly ARS program has developed sugar beet double haploid breeding lines such as KDH4-9 (R) exhibiting strong resistance to BCTV. When KDH4-9 was crossed with the highly susceptible (S) line, KDH19-17, the BCTV resistance trait segregated into three distinct resistance groups in the F2 population. We used graded-pool sequencing to rapidly map QTLs through whole-genome sequencing and bulked-segregant analysis. Graded-pool sequencing of individuals from the R and S groups (segregating population) and parents when combined with transcriptome analysis, revealed resistance associated QTLs primarily localized in chromosome 3 and a few minor QTLs in chromosomes 1, and 2. Resistance related genes identified were Bevul.3G031800 (RNA transport), Bevul.1G037400 (DNA-directed RNA polymerase III), Bevul.2G154500 (amino acid transmembrane transport). The putative genomic markers associated with BCTV resistance in KDH4-9 will be valuable for future trait introgression into commercial cultivars.
Genomic regions responsible for rhizomania resistance in a mutagenic sugar beet line identified through graded-pool sequencing and transcriptome analysis.
LONG, EVAN M.*1, RAJTILAK MAJUMDAR1, CARL A. STRAUSBAUGH1, ERIC D. VINCILL1, and IMAD A. EUJAYL1, 1USDA-ARS Northwest Irrigation & Soils Research Lab, 3793 North 3600 East, Kimberly, ID 83341, USA.
Abstract View Article
Rhizomania in sugar beet caused by Beet necrotic yellow vein virus (BNYVV) vectored by the soilborne plasmodiophorid, Polymyxa betae, significantly reduces yield and sucrose content globally. Genetic resistance to rhizomania in commercial cultivars is obtained through the presence of Rz1 and Rz2, resistant genes. Recently, Rz mediated resistance in commercial cultivars have shown some compromise under field evaluations. Through EMS mutation and genetic selection, sugar beet breeding line KEMS12 was developed, which is highly resistant (R) to rhizomania. KEMS12 when crossed with a rhizomania susceptible (S) line, KPS24, showed segregation of rhizomania resistance trait in the F2 population. We implemented a quantitative trait locus (QTL) mapping method, graded-pool sequencing, to rapidly map QTLs through whole-genome sequencing of graded-pool samples from F2 progeny using bulked-segregant analysis. Graded-pool whole genome sequencing combined with transcriptome analysis identified putative resistance associated QTLs primarily localized in chromosomes 2, 6, and 8. Candidate genes whose expression showed similar pattern between the highly R and S individuals in the segregating population and parents, included Bevul.2G022500 (small nucleolar RNA-associated protein), Bevul.2G022400 (spliceosome complex disassembly), and Bevul.8G104700 (protein phosphatase inhibitor). The putative rhizomania resistance markers identified in KEMS12 will be greatly valuable for future trait introgression into commercial cultivars.
Virus Yellows resistant/tolerant varieties for sustainable sugar beet production, when seed treatments are banned.
LENNEFORS, BRITT-LOUISE*1, EMMA THORELL1, KARINE HENRY2, TYLER RING3, and HENDRIK TSCHOEP1, 1United Beet Seeds, Industriepark 15, 3300 Tienen, Belgium, 2United Beet Seeds, 3 rue Florimond Desprez, Cappelle-en-Pévèle, France, 3United Beet Seeds, 1020 Sugar Mill Road, Longmont, CO 80501, USA.
Abstract View Article
Virus Yellows is caused by several aphid-transmitted virus species. For many years, the disease was successfully controlled by neonicotinoids, but as insecticides were banned in the EU in 2018, Virus Yellows has again evolved to become one of the major threats to the sugar beet crop in Europe. Breeding has continued for many decades with the development of hybrids with resistance and/or tolerance to Virus Yellows. Evaluation of new lines and hybrids is continuously done in greenhouse, semi-field and field trials for both single virus infections, as well as multiple virus infections. Tests are done in quarantine conditions to check the sustainability of resistance/tolerance by using several virus isolates with different origins. New hybrids are successfully developed with low or no yield gap. Additionally, hybrids with green canopy also have a robustness against different leaf diseases.
Finding solutions against viral yellows in Europe without phytosanitary products.
LAUDINAT, VINCENT*1 and FABIENNE MAUPAS*1. 1Institut Technique de la Betterave ITB-France, 45 rue de Naples 75008 Paris.
Abstract View Article
In Europe, public authorities, in response to very strong societal expectations, systematically encourage the reduction of synthetic phytosanitary product uses. Thus, in 2018 in France, all the neonicotinoid range insecticides including products with similar action modes, were, in order of the preservation of foraging insects, completely banned in sugar beet, and on all other crops. In 2020, the various viruses of beet yellows, following an explosion of aphid vector populations, no longer controlled by neonicotinoid insecticides, developed strongly on almost all of the 400,000 French sugar beet hectares, leading to a loss of 30% of production at the national level, impacting the entire economy of our French sugar beet sector, while questioning its sustainability in the most affected regions. In 2021, a national research program partially funded by the government focusing on solving these recurring viral threats was launched. This program was intended to be without restriction of scientific approach, including new prospects for the control of insect populations such as the repulsion of aphid vectors using smells. The first phase of this program (PNRI) started in 2021 to identify new solutions to fight viral yellows has been completed and we have moved on to the operational stage of deploying these solutions in real crop conditions. To do this, we rely on an original network of about sixty pilot farms deployed throughout the country to conduct experiments in real growing conditions in the different production contexts sugar beets are grown in France. The control strategy is currently based on 4 pillars: reducing viral reservoirs through prophylaxis, reducing the presence of aphids, maximizing biological regulation and increasing varietal tolerance to viruses. We propose to present during the ASSBT congress the research approach and the results obtained for each lever tested. The first bases of reasoning for the combination of these levers will also be discussed, as well as the obstacles to adoption by farmers.
Speed breeding practices in sugar beet: An application to Yellowing disease resistant breeding.
KURODA, YOSUKE*1, HIROAKI MATSUHIRA1, TSUBASA NARIHIRO1, KAZUYOSHI KITAZAKI2, and TOMOHIKO KUBO2, 1Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization, 9-4 Shinseiminami, Memuro, Kasai, Hokkaido 082-0081, Japan, 2Hokkaido University, Laboratory of Genetic Engineering, Research Faculty of Agriculture, Sapporo 060-8589, Japan.
Abstract View Article
Biennial sugar beet (Beta vulgaris) can ensure high sugar production in the field by continuing to grow vegetatively until harvest, but they require several months of vernalization period to induce flowering to obtain seeds. This absolute vernalization requirement is a decisive factor in the lifecycle of a biennial plant. Interestingly, two strains have been found in biennial sugar beet that can flower quickly under a 24-hour condition without being exposed to cold temperatures. We named the flowering characteristics ‘BLOND’ (bolting by longer than natural daylength) and made crossings between strains of the BLOND and normal biennial to obtain segregating generations of F1, F2 and BC1F1. Their flowering rates were investigated under a 24-hour day length by supplementing light at night both in summer and winter. Those results suggested that they were controlled by a few strong genes with dominant effect. Thus, the flowering tendency of BLOND can be applied for speed breeding. Furthermore, as a case study, this study demonstrates that by simultaneously using its speed breeding platform based on BLOND and the already developed DNA marker selection, yellowing disease resistance breeding can be achieved in a short period of time.
The de novo sequencing, assembly, annotation, gene expression, and public database housing genomic dataset sequences for the sugar beet root maggot Tetanops myopaeformis, TmSBRM_v1.0.
KLINK, VINCENT*1, SUDHA ACHARYA2, MUHAMMAD MASSUB TEHSEEN3,4, CHENGGEN CHU3, and NADIM W. ALKHAROUF2, 1USDA-ARS-NEA-BARC, Molecular Plant Pathology Laboratory, Building 004, Room 122, BARC-West, 10300 Baltimore Ave., Beltsville, MD 20705, 2Towson University, Department of Computer and Information Sciences, Towson, MD, 21252, USA, 3USDA-ARS-NA- Northern Great Plains Research Laboratory, 1307 N 18TH ST Northern Crop Science Laboratory, Fargo, ND 58102, USA, 4North Dakota State University, Department of Plant Sciences, Fargo, ND 58102, USA.
Abstract View Article
The sugar beet root maggot (SBRM), Tetanops myopaeformis (von Röder), is a devastating insect pathogen of sugar beet (SB), Beta vulgaris, ssp. vulgaris (B. vulgaris), an important food crop, while also being one of only two plants globally from which sugar is widely produced, and accounting for 35% of global raw sugar with an annual farm value of $1 billion in the United States alone. The limited natural resistance of B. vulgaris necessitates an understanding of the SBRM genome to facilitate generating knowledge of its basic biology, including the interaction between the pathogen and its host(s). Presented is the de novo assembled draft genome sequence of T. myopaeformis isolated from field-grown B. vulgaris in North Dakota, USA. Additional work includes its annotation, gene expression, and a web base to access the available information. The SBRM genome sequence TmSBRM_v1.is valuable for molecular genetic marker development to facilitate host resistance gene identification and knowledge, and aid in agronomic improvement and management of sugar beet for stakeholders while also providing information on the relationship between the SBRM and climate change.
Decoding the biotrophic phase of Cercospora beticola through functional characterization of effectors.
JASWAL, RAJDEEP*1,2, LEONARD BARNABAS EBINEZER1,2, GARY SECOR2, MELVIN D. BOLTON1,2 and NATHAN WYATT1,2, 1United States Department of Agriculture – Agricultural Research Service, Sugarbeet and Potato Research Unit, Edward T. Schaffer Agricultural Research Center, 1307 18th Street North, Fargo, ND 58102, 2North Dakota State University, Department of Plant Pathology, Walster Hall 306, Fargo, ND 58102.
Abstract View Article
Cercospora beticola, causal agent of Cercospora leaf spot (CLS), is the most prominent foliar disease of sugarbeet. The infection caused by C. beticola can significantly deteriorate sugarbeet foliar tissue leading to severe yield losses. Despite being a pathogen of high economic importance, very limited information is available regarding the molecular mechanisms deployed by C. beticola to manipulate the sugarbeet host. Recently, we sequenced a large globally diverse population of C. beticola to identify effector genes that are conserved among the species and leveraged RNAseq data to select potential effectors that were transcriptionally active during the biotrophic phase of C. beticola infection. Candidate effectors were transiently expressed in Nicotiana benthamiana using to screen for cell death inducing activity. Majority of the selected candidate did not induce any cell death inducing phenotype in N. benthamiana leaves however four effectors demonstrated cell death suppression activity. Effectors suppressing cell death were also able to suppress the production of reactive oxygen species (ROS) induced due to host defense pathways activation. Further, In planta localization studies of fluorescently tagged candidates highlighted chloroplast to their potential target site. The current study provides a framework for understanding the function of C. beticola biotrophic effectors and their role in plant defense manipulation.
Unmanned aerial vehicle-based high-throughput phenotyping in sugar beet fields.
KIM, JAMES Y.*1 and CHENGGEN CHU1, 1USDA-ARS Edward T. Schafer Agricultural Research Center, Sugarbeet Research Unit, Fargo, ND, 58102.
Abstract View Article
Germplasm trait evaluation is essential to identify genetic sources for sugarbeet breeding. Accurate phenotypic assessment requires plants grown in replicate field trials under different environments. Manually scoring traits of germplasm at a large scale in the field is laborious and inconsistent. Utilizing computer vision-driven phenotyping can expedite the breeding process pipeline and deliver consistent phenotypic metrics thus becomes an urgent need. Unmanned aerial vehicle (UAV)-based high-throughput plant phenotyping is proposed in this study to characterize the performance of sugarbeet traits using an in-house image analytic software. Experiment was conducted in two sugarbeet root maggot nurseries close to St. Thomas and Buxton, ND, respectively. A UAV was deployed to map the sugarbeet field with various altitudes (32ft and 100ft) and overlaps (20%, 50%, and 80%) to evaluate image quality and stitching performance. Plot-level metrics is calculated by defining an area of interest (AOI) of the field boundary and processing geofenced pixels for vegetation and leaf area indices in a sub-AOI aligned with each plot using a gridding method through segmentation, filtering, masking, and clustering. The result indicated that UAV-based crop mapping integrated with open-source analytic software can perform high-throughput phenotyping in an effective manner and can facilitate the data processing pipeline in plant breeding and biotechnology. The system is scaled up to direct geo-referencing of UAV images for improved mapping time and coverage.
Several faces of Colletotrichum on sugar beet.
HANSON, LINDA E.*1, JAN BYRNE2, DOUGLAS H. MINIER3, and JAIME F. WILLBUR3, 1USDA-ARS, 612 Wilson Road, East Lansing, MI 48824, 2Michigan State University, Plant & Pest Diagnostics, 578 Wilson Road, East Lansing, MI 48824, 3Michigan State University, Dept. of Plant, Soil and Microbial Sciences, 612 Wilson Road, East Lansing, MI 48824.
Abstract View Article
Fungi in the genus Colletotrichum have been detected on sugar beet in several regions. In many areas, no symptoms have been reported. However, in recent years we have observed a variety of symptoms caused by different Colletotrichum species on beet. A seedling damping-off was first found in Michigan in 2016, with plant symptoms primarily of stunting and discoloration. Few beets died, but growth was delayed. This has continued to be present, with samples found around the Michigan production region every year that seedling samples were collected since that time; one of two Colletotrichum species has been confirmed for each sample. In addition to seedling damage, unusual foliar symptoms were observed in 2016, with oblong lesions on the petiole of beets. From these, Colletotrichum incanum was isolated consistently. While this symptom has only rarely been observed, in foliar inoculations it was observed that symptoms similar to those caused by Phoma betae formed on the blades of leaves. The lesions were large, round with irregular borders, and often had at least one darker ring, but showed less distinct browning than generally observed with Phoma leaf spot. Two species of Colletotrichum could be isolated from lesions on the leaf blade, C. incanum or C. lineola. Both caused similar lesions in leaf inoculations. A third Colletotrichum has been isolated from beet roots. Colletotrichum coccodes was first isolated in Idaho but a similar Colletotrichum sp. has now been found on beet in Michigan. This causes shallow lesions on the root that have many small “black dots” (leading to the disease name on potato). It has been reported to increase susceptibility to some other fungi in potato but its impact in beet is unknown. Anthracnose caused by Colletotrichum dematium had been reported in Japan, with similar symptoms to the foliar and seedling damage observed in the US. This pathogen also could cause loss of flowers in seed production as well as infesting seed. It is unknown whether any of the recently identified Colletotrichum can be associated with seed production issues and their overall impact is still being determined.
Evaluating BEETcast™, a disease forecasting model, for timing fungicide sprays with CR+™ varieties.
GUZA, COREY J.*1 and ZACHARY YOUNG1, 1Michigan Sugar Company, 122 Uptown Dr., Suite 300, Bay City, MI 48708.
Abstract View Article
Varieties are available that have a strong tolerance to Cercospora Leafspot (CR+™). In the Michigan Sugar Company growing region, growers have sprayed fungicides for Cercospora Leafspot using calendar dates and a disease forecasting model BEETcast™. With the stronger variety tolerance to Cercospora Leafspot studies were conducted to determine the optimal spray interval by variety. Multiple varieties with a range of Cercospora Leafspot tolerance from high to low were tested by spraying fungicides at intervals of every 15 days, 35 disease severity values (DSV’s) and 55 DSV’s. Varieties with strong Cercospora Leafspot tolerance performed well when sprayed every 55 DSV’s. Varieties with weaker Cercospora Leafspot tolerance needed to be sprayed every 15 days or at 35 DSV’s.
Leaf shape and size are highly heritable in sugar beet breeding populations and may be a valuable source of diversity for improving resiliency in changing climates.
DENEEN, AIDAN1, DAN CHITWOOD1, PRANIT AHUJA1, and RACHEL P. NAEGELE*2, 1Michigan State University, East Lansing, MI 48824, 2USDA-ARS 1066 Bogue St. East Lansing, MI 48824.
Abstract View Article
Sugar beet leaf morphology and plant architecture has largely been unselected over the past 150 years of breeding. Yet leaf characteristics and plant architecture have an impact on canopy closure and air flow, drought and cold tolerance, light capture, and ultimately sucrose accumulation in roots. Using greenhouse grown plants, we evaluated leaf morphology [e.g. color, size, shape, venation patterns] for 269 sugar beets representing 27 heterogenous accessions and 18 genetic families from the USDA ARS East Lansing sugar beet breeding program through a python-based Procrustean morphospace pipeline. Significant variability was observed within and among beet accessions and families for total leaf area, width, length, shape, and color. The greatest variability in shape was observed along the base of the leaves near the petiole attachment and the upper 1/3 of the leaf blade. Using a genome-wide association approach, SNPs associated with total leaf area and shape were identified.
Effects of synonymous and nonsynonymous Cyp51 mutations on DMI resistance in Cercospora beticola.
COURNEYA., ISAAC*1, NATHAN WYATT1,2, GARY A. SECOR2, VIVIANA V. RIVERA-VARAS2, and MELVIN D. BOLTON1,2, 1USDA-ARS ETSARC, Fargo ND 1307 18th St N, 2North Dakota State University, Dept. of Plant Pathology, 355 Walster Hall, Fargo ND.
Abstract View Article
Cercospora leaf spot (CLS), caused by the fungal pathogen Cercospora beticola, is the most economically important disease of sugarbeet worldwide. One of the primary means of combatting this disease is the timely application of fungicides. Demethylation inhibitors (DMIs) are a class of fungicide that target Cyp51, a key enzyme in the synthesis of an essential fungal cell membrane component called ergosterol. While DMIs are important for managing CLS, resistance to these fungicides has been observed. Previous work in our lab has shown that resistance to DMIs is highly correlated with synonymous and non-synonymous mutations in the Cyp51 gene. One such mutation, L144F, is found in two codon variants TTC and TTT, where TTC is associated with resistance and TTT is associated with sensitivity even though both codons encode phenylalanine. Notably, resistance is also strongly associated with the synonymous mutation E170. We have identified five Cyp51 haplotypes exhibiting different combinations of these mutations. To improve our understanding of the effects of silent mutations on DMI resistance, we have undertaken a variety of studies to characterize the role of this gene in mediating DMI resistance. The results and potential implications of these studies will be presented.
Exploring gene sources for sustainable improvement of sugarbeet.
CHU, CHENGGEN*1, MUHAMMAD MASSUM TEHSEEN2, LISA PREISTER1, AHMET BARKAN BOSTAN2, JOLENE ADDINGTON2, HEE KYUNG LEE1, NATHAN WYATT1, MELVIN D. BOLTON1, KAREN FUGATE1, VANITHARANI RAMACHANDRAN1, MARK BOETEL3, ERIC BRANCH4, and XUEHUI LI2, 1USDA-ARS, Edward T. Schafer Agricultural Research Center, Sugarbeet Research Unit, Fargo, ND 58102, 2North Dakota State University, Department of Plant Sciences, Fargo, ND 58102, 3North Dakota State University, Department of Entomology, Fargo, ND 58102, 4North Dakota State University, Department of Plant Pathology, Fargo, ND 58102.
Abstract View Article
Sugarbeet (Beta vulgaris L. ssp. vulgaris Doell.) was originally selected from white fodder beet in the 1780s and is now an important source of sucrose production. The relatively short history of sugarbeet leads to its narrow genetic base and limited genetic resources for sugarbeet trait improvement. Exploring gene sources for sustainably improving sugarbeet is urgent to increase sugar productivity as well as enhance crop resilience to climate change. The USDA-ARS sugarbeet genetics program at Fargo, ND has evaluated 1,936 sugarbeet germplasm that are largely maintained by the U.S. National Plant Germplasm System (NPGS). Genetic diversity analysis using SNPs covering the whole genome identified wild sea beet (B. vulgaris ssp. maritima (L.) Arcang., the progenitor of all cultivated beet) accessions that were genetically distinct from cultivated beet, indicating their great potential to broaden the sugarbeet genetic base. Evaluation of disease and insect resistance in the sea beet identified accessions showed a high level of resistance to Cercospora leaf spot (causal organism: Cercospora beticola) and sugarbeet root maggot (Tetanops myopaeformis). Our ongoing study aims to detect genome regions of sea beet associated with resistance to diseases and insects, and then develop markers for efficient introgression of these traits. Taken together, our research identified important genetic resources that can be used to increase sugarbeet sustainability and will facilitate the development of new varieties to improve sugar productivity.
The sugarbeet phyllosphere harbors bacteria capable of inhibiting Cercospora beticola, the causal agent of Cercospora leaf spot.
CHRISTENSON, MADISON J.*1,2, DANIEL BACK2, MARI B. NATWICK1,2, LORENA I. RANGEL2, GARY A. SECOR1, and MELVIN D. BOLTON 1,2, 1North Dakota State University, Department of Plant Pathology, Fargo, ND, USA, 2Edward T. Schafer Agricultural Research Center, Northern Crop Science Laboratory, United States Department of Agriculture, Fargo, North Dakota, USA.
Abstract View Article
Cercospora beticola is a fungal pathogen of sugarbeet (Beta vulgaris L. ssp. vulgaris) that causes Cercospora leaf spot (CLS). Unfortunately, C. beticola has proven adaptable to current management practices due in part to high levels of genetic diversity found in most field populations. The phyllosphere is composed of the above ground areas of a plant that serve as a habitat for microbes, which can be influenced by abiotic and biotic stressors. We hypothesized that the sugarbeet phyllosphere harbors bacterial communities that may have properties to ward off plant pathogens like C. beticola. To that end, CLS-infected sugarbeet leaves were utilized to create a collection of culturable epiphytic bacteria. A total of 447 bacterial isolates were sequenced from CLS-resistant and -susceptible cultivars across five sampling timepoints. Cercospora beticola isolates were challenged with bacteria from the collection to ascertain antagonistic phenotypes. The assays revealed that Burkholderia contaminans reduces fungal growth without direct mycelial contact or via volatile compounds. Bacterial and fungal RNA-seq was conducted to provide insight into the interactions between B. contaminans and C. beticola. Secondary metabolite molecules putatively involved with C. beticola growth inhibition will be discussed. Identification of the compound(s) responsible for inhibition could provide us with a new management tool for CLS.
Strategies for successful management of Cercospora leaf spot and emerging foliar diseases in sugar beet.
CHANDA, ASHOK K.*1,2 and AUSTIN K. LIEN1,2, 1University of Minnesota, Dept. of Plant Pathology, 1991 Upper Buford Circle, St. Paul, Minnesota, 55108, 2University of Minnesota Northwest Research & Outreach Center, 2900 University Avenue, Crookston, Minnesota, 56716.
Abstract View Article
Cercospora leaf spot (CLS), caused by Cercospora beticola, continues to be a major threat to sugar beet production in the USA and other sugar beet growing areas of the world. CLS epidemics have become more frequent partly due to favorable weather conditions for CLS development, widespread insensitivity of C. beticola populations to commonly used fungicides and adaptation of C. beticola to new hybrids with extreme tolerance to CLS (CR+ hybrids). In a short span of 3 years of research with CR+ hybrids, we observed initiation of CLS earlier in the season and CLS severity in nontreated control plots is gradually reaching levels of economic threshold. Based on these observations, timely fungicide application remains critical for effective management of CLS irrespective of the level of tolerance of the varieties to CLS. Our research trials demonstrated that utilization of multiple modes-of-action fungicides in a spray program is essential to maintain effective CLS management especially when C. beticola is known to be insensitive to some fungicides. As the proportion of C. beticola isolates that can infect CR+ hybrids increase, it will be difficult to justify reduced number of fungicide sprays on CR+ hybrids. We also observed emergence of Alternaria leaf spot (ALS) and Stemphylium leaf spot (SLS) diseases based on the diagnostic samples received in our laboratory since 2021. In 2024 a field trial was set up to evaluate the efficacy of standard CLS fungicide spray program against ALS and SLS on three different sugar beet hybrids. If the fungicides currently used for CLS do not have the same efficacy in managing ALS and SLS, incidence of these diseases is expected to increase as the acreage for CR+ hybrids expands further in MN and ND.
Management of Cercospora Leaf Spot on CLS improved sugarbeet varieties.
BHANDARI, SUNIL*1, SUSHMITA KALIKA-SINGH1, ERIC BRANCH1, LUIS DEL RIO1, and MOHAMED F.R. KHAN1, 1North Dakota State University, Dept. 7660, PO Box 6050, Fargo, ND, 58108-6050.
Abstract View Article
Cercospora leaf spot (CLS), caused by the airborne fungus Cercospora beticola, is the most destructive and economically significant foliar disease affecting sugar beet. Yield losses can exceed 40% when environmental conditions favor disease development. While CLS is typically managed with fungicides on a timely basis, excessive use not only adds to production costs but also increases the selection pressure for fungicide resistance in the pathogen. Since 2021, improved sugar beet varieties with the BvCR4 gene that confers higher CLS tolerance (CR+) have become available to producers. Since there is a history of C. beticola populations overcoming the effectiveness of fungicides, we conducted field research to observe the ability of C. beticola to infect CR+ varieties in the presence and absence of fungicides. Field research was conducted from 2022 to 2024 in Moorhead, Minnesota, using two CR+ and one standard susceptible variety, to evaluate the effectiveness of these improved varieties against C. beticola and revealed that their tolerance is beginning to break down. Disease infection and severity was very low and limited to a few plants in 2022. The frequency of C. beticola infection and disease severity increased significantly in 2023 and 2024. Initially, the disease progressed slowly, with the first spots appearing 2-3 weeks later than in a standard susceptible variety; however, disease progression eventually matched that of these susceptible varieties. In untreated CR+ controls, the area under the disease progress curve (AUDPC) steadily increased from 0.7 and 1.3 to 15 and 24 (P < 0.05), indicating a decline in CLS tolerance over time. In contrast, fungicide applications initiated on a calendar basis, at disease onset, and as needed significantly reduced the disease severity, with AUDPC values of 2.9, 5.9 and 6.1 respectively, (P < 0.05), compared to the untreated control (AUDPC = 19, P < 0.05). Although the calendar-based application yielded the lowest AUDPC, recoverable sugar (RSA) did not differ significantly compared to early-start or as-needed applications (P < 0.05), with as needed approach requiring fewer fungicide applications. These results indicates that the fungus, over a period of 3 years, was able to overcome the natural durability of the improved CR+ varieties, underscoring the importance of timely and judicious fungicide application to prevent significant economic losses and help sustain fungicide effectiveness over the long term.
Characterization of the resistance protein Rz2 and its interaction with the avirulence protein triple gene block I from beet necrotic yellow vein virus.
BENJES, KRISTIN1, MARK VARRELMANN*1 and SEBASTIAN LIEBE1, 1Institute of Sugar Beet Research, Department of Phytopathology, Holtenser Landstraße 77, 37079 Göttingen, Germany.
Abstract View Article
Beet necrotic yellow vein virus (BNYVV) causes the devastating disease rhizomania in sugar beet. For decades, the disease has been successfully controlled by using the resistance genes Rz1 and Rz2. However, the spread of Rz1-resistance breaking populations emphasizes the importance of Rz2. The resistance protein encoded by Rz2 has been identified as a nucleotide binding (NB) and leucine-rich-repeat receptor (Capistrano-Gossmann et al., 2017). In a transient assay in Nicotiana benthamiana, Rz2 was shown to recognize BNYVV triple gene block I (TGB1) as the corresponding avirulence protein leading to a hypersensitive response (HR) with cell death (Wetzel et al., 2021). Colocalization experiments and interaction assays were conducted to characterize the interaction. A mutation was introduced into the phosphate binding loop inside the NB domain of Rz2 to abolish the rapid HR for fluorescence visualization. Coexpression of this K201A Rz2 mutant fused to GFP and BNYVV TGB1 fused to mRFP demonstrated that Rz2 and BNYVV TGB1 colocalize in the cytoplasm and nucleus. Manipulation of the subcellular localization by fusion of Rz2 and BNYVV TGB1 with a nuclear localization signal (NLS) resulted in a greatly reduced HR, whereas fusion of both with a nuclear export signal (NES) did not affect the HR, suggesting that the cytoplasmic distribution of Rz2 and BNYVV TGB1 is important for Rz2 mediated resistance. In a yeast-two hybrid (Y2H) assay, no direct physical interaction between Rz2 and BNYVV TGB1 was detected. Similar to Y2H, bimolecular fluorescence complementation (BiFC) with the K201A Rz2 mutant failed to show a direct interaction in planta. Missing evidence for a direct interaction suggests an indirect interaction with a conserved host protein present in sugar beet as well as in N. benthamiana. Proximity labeling coupled with mass spectrometry will help to identify a potential intermediate host protein as well as other unknown interaction partners.
Physiology, Genetics, and Plant Pests - Poster
Characterization of pathogens causing leaf spot diseases of sugarbeet in Michigan.
WEEDON, EMILY J.*1, LINDA E. HANSON1,2, ALEXANDRA P. HERNANDEZ1, SARAH RUTH1 and JAIME R. WILLBUR1, 1Michigan State University, 612 Wilson Road, 35 Plant Biology Lab, East Lansing, MI 48824, 2USDA-ARS, 612 Wilson Road, 37 Plant Biology Lab, East Lansing, MI 48824.
Abstract View Article
Sugarbeet account for over half of all sucrose production nationwide in the United States. Domestic sugarbeet production is challenged by a variety of diseases and pests impacting yield, quality, and storability. Cercospora leaf spot, the most important of these foliar diseases, has had issues with fungicide resistance reported in several sugarbeet producing regions. In addition, reports of increased prevalence and fungicide resistance within the historically minor disease, Alternaria leaf spot, has indicated a need for improved management. This study aims to characterize current Alternaria spp. impacting Michigan sugarbeet crops and, further, to investigate the stability of fungicide resistances within isolates of causal agents, Alternaria spp. and Cercospora beticola. Isolates were collected from symptomatic sugarbeet leaf samples and characterized using a detached-leaf assay for virulence. An initial spiral gradient dilution screen was conducted to assess responses to common fungicide classes, including demethylation inhibitors (DMI), benzimidazoles, quinone outside inhibitors (QoI), and organo-tin fungicides. From two years of sampling, approximately 60-90% of lesion area caused by isolates of Alternaria spp. varied significantly from the controls on an Alternaria-susceptible commercial variety. Characterized cultures of eight virulent Alternaria spp. isolates and seven virulent C. beticola isolates were exposed to cold temperatures (20, 4, and -20°C), mimicking mild to extreme overwintering conditions. Isolates were screened after two weeks and then every subsequent month for seven months to monitor potential between-season shifts in fungicide sensitivity. Following seven months of cold exposure, no biologically significant shifts in sensitivities to two DMIs, difenoconazole, or tetraconazole, or a benzimidazole, thiophanate-methyl, were observed for any isolates. However, tin sensitivities for both pathogens increased significantly over time (P < 0.05), which indicates that a return to tin sensitivity could be observed over several months regardless of temperature. None of the fungicides screened displayed meaningful shifts due to environmental factors, however, second year of data is continuing for both pathogens. These studies will help to inform in- and between-season management considerations for major sugarbeet leaf spot pathogens.
Development of a greenhouse screening assay to evaluate sugar beet germplasm for resistance to Beet Curly Top virus (BCTV).
VINCILL, ERIC D.*, LAURIE K. JACKSON and CARL A. STRAUSBAUGH, USDA-ARS, NWISR Sugar Beet Breeding and Research Laboratories, Kimberly, ID 83341.
Abstract View Article
Beet curly top virus (BCTV) in sugar beet is an important yield limiting disease problem in semi-arid production areas of the western U.S. BCTV is transmitted exclusively by the insect beet leafhopper, Circulifer tenellus. Resistant sugar beet cultivars remain a primary control measure, however most commercial cultivars only contain low to moderate levels of resistance because the resistance is thought to be quantitatively inherited and is difficult to maintain in parental lines used to create commercial hybrids. This is further complicated by the fact the mutation rate of BCTV far exceeds the breeding timeline of sugar beet. Methods to screen sugar beet germplasm for new and novel traits of resistance is complicated by the fact that the virus is transmitted by the beet leafhopper which is difficult rear and maintain in the laboratory setting. Field evaluations for BCTV resistance in sugar beet has traditionally been limited to areas in the western U.S. that are conducive to beet leafhopper habitats during the growing season and carry the BCTV virus. Beet leafhoppers are also known to co-currently carry and transmit multiple variants of the BCTV virus (Severe, Cal-Logan, etc.) at the same time which further complicates the interpretation of screening outcomes in field trials. To expedite the screening of sugar beet germplasm for new and novel sources of resistance traits, we have developed an agroinfiltration shuttle vector system (Agrobacterium t.) that bypasses the need for the beet leafhopper to transmit the BCTV virus into sugar beet seedlings. Once agroinfiltrated into the plant, the BCTV full-length virus is mobilized and replicated de novo resulting in systemic and persistent BCTV disease symptoms in the seedling. Methods to use this agroinfiltration vector system as a high-throughput method to screen USDA sugar beet plant introductions (PI lines) or other sugar beet lines of interest will be discussed.
Always on the watch: supplementing networks with technology to monitor changing sugar beet pathogen and pest composition and distribution.
PETERSON, JENNIFER J.*1, HARALD KEUNECKE2 and FRANCISCO E. MENDEZ CASTRO2, 1KWS SEEDS, LLC, 1325 Valley View Rd, Shakopee, MN 55379, U.S.A, 2KWS SAAT SE & Co. KGaA, Grimsehlstrasse 31, 37574 Einbeck, Germany.
Abstract View Article
The monitoring of pathogens and pests has become increasingly critical. This urgency arises not only from the adaptation of pathogens to control measures but also from the heightened movement driven by the global economy and climatic instability. Compounding this issue is the reduced availability of chemical treatments, particularly in Europe, to manage pest outbreaks. This situation underscores the pressing need for sugar beet varieties with stable resistance to diseases and pests. Fortunately, advances in expertise, data collection, and technology are helping to combat these problems. Networks connecting farmers, sales representatives, breeders, and researchers can provide early warning signals of potential problems from unusual observations in the field. Digital technologies including drones and camera traps provide large and small views of the field that were previously unavailable. Molecular analysis allows us to quickly identify changes in pathogen populations that once required a time-consuming process of growing test plants with characterized resistances. GIS technology enables us to monitor high pressure areas and shifts in pathogens/vectors over time. Using a centralized database system, we can track changes in diseases/pests of concern both in the United States and abroad. At KWS, current monitoring efforts are focused on pathogens and vectors of high concern including Cercospora Leaf Spot, leafhoppers, aphids, and Beet Necrotic Yellow Vein Virus. However, our system is flexible enough to pivot if a new major threat is identified. By monitoring both established and emerging pathogen and insect pests, as well as distribution patterns, we are better able to determine what new strategies are needed to ensure stability of resistance traits and continued sugar beet profitability. To that end, vigilance can be a powerful tool, and everyone involved in sugar beet production should be encouraged to be alert to potential concerns and empowered to communicate their observations.
Using nanoparticles to enhance fungicide efficacy for management of Cercospora leaf spot of sugar beet.
NEUBAUER, JONATHAN*1, RAJ HAZRA2, MOHI QUADIR2, CHENGGEN CHU1, MELVIN BOLTON1 and LONG JIANG2, 1USDA-ARS, Edward T. Schafer Agricultural Research Center, Sugarbeet Research Unit, Fargo, ND 58102. 2North Dakota State University Department of Mechanical Engineering, Fargo, ND 58108.
Abstract View Article
Cercospora leaf spot (CLS) of sugar beet is a devastating disease caused by the fungal pathogen Cercospora beticola. Management of CLS mainly relies on the timely application of fungicides. However, many populations of C. beticola around the world have become resistant to most fungicide classes. Therefore, the objective of this study was to use cellulose nano fibers (CNFs) to enhance efficacy of fungicide for disease management. Five treatments were used; inoculum only, CNF-Cu at 2 mg/ml, CNF-CU at 1mg/ml, CNFs and buffer only. These treatments were applied to 5-week-old sugar beet plants. Three days later, C. beticola was inoculated at 10^5 spores per ml and disease severity was evaluated two weeks later to evaluate efficacy of CNF-Cu treatments. Disease inoculation of all CNF-Cu treated plants showed significantly less CLS than non-treated plants or plants treated with CNF or buffer alone, which suggests CNF-Cu can inhibit disease development on sugar beet leaves. An additional potential benefit of using CNF-Cu is the nano scaled cellulose fibers can tightly adhere to plant leaves and therefore may avoid rainfall wash off to provide longer defense against fungus. Future greenhouse experiments will cover multiple high and low concentrations of CNF-Cu as well as rainfall simulated events to provide great insight for future field applications. This study shows that CNFs based nano technology has the potential to be a useful management strategy for Cercospora leaf spot.
Simulated canopy loss due to Cercospora leaf spot.
NEHER, OLIVER T.*, CLARKE ALDER, EMILY CORGATELLI and TYLER MORRIS, Amalgamated Sugar Company, PO Box 1766, Nyssa, OR 97913.
Abstract View Article
Cercospora beticola, causal agent of Cercospora leaf spot (CLS), can have destructive effects on the sugar beet canopy if left uncontrolled. Some studies have investigated the effects of partial or full defoliation as a single event caused by hail or insect damage, but we are not aware of studies looking at multiple canopy losses such as what CLS can cause throughout the growing season. In 2024, a study was conducted comparing 5 single-date to 4 multi-date defoliations. Defoliations were conducted beginning the week of July 8 and continued every two weeks. Plots were mechanically defoliated using a hedge trimmer without injuring the crown tissue. Canopy coverage, internal beet root and ambient temperature (delta T), and yield data were collected at the end of the growing season. Earlier defoliations (7/8, 7/22) showed the greatest difference for delta T. Plots defoliated multiple times on 7/8 followed by (fb.) 8/5 and 8/22 fb. 8/19 showed a similar trend. Strong correlations between percent canopy coverage and delta T were observed with late season defoliations (8/19, 9/2, 8/5 fb. 9/2, 7/8 fb. 8/5 fb. 9/2) resulting in smaller canopy coverage and reduced delta T. Increased internal root temperature due to defoliation can potentially hamper harvest operations based on existing heat policies. All treatments had significantly lower sucrose content (% sucrose) when compared to the untreated control. Percent sucrose was less affected by single defoliations with earlier events resulting in higher % sucrose than later defoliations. The opposite was observed for yield with earlier defoliations showing numerically lower yields than later defoliations. However, multiple defoliations nearly always resulted in the lowest % sucrose and yield of all treatments. This study demonstrates the importance of a healthy sugar canopy throughout the growing season and the need for effective control measures for CLS.
Development of a mid-density AgriSeq genotyping chip for sugar beet and its relatives.
NAEGELE, RACHEL P.*1, EVAN LONG2, KEVIN DORN3, PIERGIORGIO STEVANATO4 and IRWIN GOLDMAN5, 1USDA-ARS, 1066 Bogue St., East Lansing, MI 48824, 2USDA-ARS, 3793 North 3600 East, Kimberly, ID 83341, 3USDA-ARS, 210 Centre Avenue, Ft. Collins, CO 80526, 4University of Padova, Viale Universita 16 Legnaro, Italy, 5University of Wisconsin, 1575 Linden Drive, Madison, WI 53706.
Abstract View Article
Cost-effective and standardized genotyping platforms are essential for the rapid improvement of crop species. For many major crop species, such as wheat, soybean, corn, potato, etc. these resources are available and have enabled community-wide testing and comparisons across germplasm. In sugar beet and related Beta vulgaris crops, these resources are unavailable as of yet, due to historically limited genomic resources and the high up-front costs associated with their development. Using the recently released EL10 genome, as well as whole genome data from Beta relatives and breeding lines, we have selected approximately 4,500 high frequency targets (SNP containing amplicons) for inclusion onto an amplicon-based SNP chip (AgriSeq chip). These targets include those associated with disease resistance to major pathogens such as Rhizoctonia and Cercospora, sugar beet quality such as sugar accumulation, as well as unassociated SNPs distributed across the genome. This resource provides a cost-effective and standardized option for genotyping large numbers of individuals and is suitable for diversity evaluation, SNP discovery, and genomic selection.
Chromosome One QTLs associated with response to bacterial leaf spot in Beta vulgaris.
MORRISON, AUDREY K.* and IRWIN. L. GOLDMAN, University of Wisconsin, Department of Plant and Agroecosystem Sciences, 1575 Linden Drive, Madison, WI 53706.
Abstract View Article
Bacterial leaf spot (BLS), caused by Pseudomonas syringae pathovar aptata (Psa), is an emerging seedborne, foliar disease affecting both table beet and Swiss chard. There is no known resistance to BLS in beet or chard. The University of Wisconsin-Madison table beet breeding program has been screening for resistance in the Beta vulgaris crop complex. A diversity panel, modified from the Wisconsin Beta Diversity Panel (WBDP) and comprised of 219 accessions from the Beta vulgaris crop complex, including sugar beet and fodder beet, was assembled and genotyped for SNP data. These accessions were screened by foliar inoculation of Psa and visually evaluated for percentage of diseased leaf tissue. Overall, sugar beet and Beta maritima accessions had the lowest BLS response (indicating high potential for sources of resistance), whereas table beet accessions had the largest range of responses, with several matching the resistance levels of sugar beet. Phenotypic means were adjusted using best linear unbiased estimates, and two different software programs, GWASpoly and GAPIT3, were utilized to conduct a genome wide association study (GWAS). An association with BLS response was detected on chromosome one in the full WBDP, explaining upwards of 21% of the variation in the phenotype. Eleven candidate genes, described and annotated in sugar beet, were associated with this QTL. Marker Chr1_61344476 may be useful in breeding for BLS resistance in members of the Beta vulgaris crop complex.
The impact of sugarbeet variety mixtures on the epidemiology of Cercospora leaf spot.
LIEN, AUSTIN K.*1,2, NATHAN A. WYATT3 and ASHOK K. CHANDA1,2, 1University of Minnesota Dept. of Plant Pathology, 1991 Upper Buford Circle, St. Paul, MN, 55108, 2University of Minnesota Northwest Research & Outreach Center, 2900 University Avenue, Crookston, MN, 56716, 3USDA-ARS, Edward T. Schafer Agricultural Research Center, Sugarbeet Research Unit, Fargo, ND 58102.
Abstract View Article
Cercospora leaf spot (CLS), an economically significant foliar disease of sugarbeet caused by Cercospora beticola, continues to be a challenge for growers. Sugarbeet varieties with high tolerance to CLS with BvCR4-mediated monogenic resistance have been commercially available to sugarbeet growers since 2020. The potential for C. beticola adaptation to these new varieties is suspected to increase with widescale adoption of these varieties. There is limited knowledge on the impact of variety mixtures on CLS epidemiology and the evolution of C. beticola populations; however, variety mixtures have been shown to reduce disease severity in other crops despite unclear effects on preserving durability of resistance genes. A field trial was conducted in 2024 in which a randomized split-plot design with four replications was used. Main plot treatments consisted of two sugar beet varieties, one BvCR4 variety (CLS rating ~2.0) and one moderately susceptible variety (CLS rating ~4.9), planted in pure stand compared to mixtures consisting of either 25:75, 50:50, or 75:25 ratios. Plots were inoculated with a mixture of CLS-infested leaves collected from both BvCR4 varieties and CLS-susceptible varieties in 2023. Fungicide spray programs were assigned as subplots within variety mixtures and consisted of a nontreated control and a reduced fungicide program with one DMI application within a three-spray program. A rigorous sampling strategy was employed to obtain representative single-conidium isolates of C. beticola from the field trial (n = 320). Significant two-way interactions were present for overall CLS severity and sucrose yield, suggesting reduced fungicide programs can be utilized in varietal mixtures as well as pure stands of a BvCR4 variety. By investigating the impact of variety mixtures and reduced fungicide applications on CLS epidemiology, we aim to guide the utilization of sugarbeet varieties with BvCR4-mediated resistance to preserve the durability of the BvCR4 gene and mitigate the prevalence of C. beticola insensitive to DMI fungicides.
Evaluating the efficacy of essential oils and botanical extracts to manage sugarbeet storage pathogens.
KANDEL, SHYAM L.1, PRABIN TAMANG2 and MALICK BILL*1,3, 1USDA-ARS, Edward T. Schafer Agricultural Research Center, Fargo, ND 58102, 2USDA-ARS, Natural Products Utilization Research, University, MS 38677, 3North Dakota State University Department of Plant Pathology, Fargo, ND 58102.
Abstract View Article
Sugarbeet (Beta vulgaris L. subsp. vulgaris) is a major crop in the U.S. for sugar production which contributes nearly 60% of the total domestic sugar production. In the U.S., sugarbeet is cultivated across nine states including California, Colorado, Idaho, Michigan, Minnesota, Montana, Nebraska, North Dakota, and Wyoming spanning approximately 450,000 hectares of planting area. In general, sugarbeet roots are harvested and stored in factory yards, outdoor piles, or in ventilated sheds for several months prior to processing. During storage, different plant pathogens can infect, colonize, and deteriorate sugarbeet roots resulting in significant sucrose loss causing economic damage as high as tens of millions of dollars each year. Therefore, reduction of postharvest pathogens and sucrose loss in sugarbeets during storage is critical to mitigate economic loss to the sugar industry. However, comprehensive research on managing postharvest diseases in sugarbeet remains limited to date. In this study, we conducted in-vitro screening of 82 essential oils (EOs) against major postharvest pathogens of sugarbeet such as Penicillium, Trichoderma, and Mucor species. EOs showed variable response to different pathogen isolates. Approximately 25 EOs showed antifungal activity against Penicillium spp.;11 EOs against Trichoderma while only one EO was active against Mucor spp. The common EO that was active against all the tested pathogens was Birch (Betula lenta) EO. Our preliminary studies showed that EOs would be effective to minimize postharvest pathogen growth and improve the storage quality of sugarbeet roots. Future studies will warrant to develop a formulation of these compounds and conduct in-vivo studies to assess their efficacy in storage disease management in sugarbeets.
Non-toxic CLS disease control of sugar beet using hot water.
KIM, JAMES Y.* and NATHAN WYATT, USDA-ARS Edward T. Schafer Agricultural Research Center, Sugarbeet Research Unit, Fargo, ND, 58102.
Abstract View Article
Cercospora leaf spot (CLS) on sugarbeet, caused by the pathogen Cercospora beticola (Cb), is a critical biotic factor that affects sugarbeet growth and leads to the decrease of sucrose quantity and quality. Fungicide applications are a common practice to manage CLS, often informed by weather-based recommendation models and occurring up to 6 times during the growing season. Such heavy fungicide usage has led to Cb developing genetic resistance to fungicides and causes environmental pollution. Non-toxic CLS management is proposed as an innovative approach for sustainable CLS disease management. Heating water to a high temperature can be as deadly as toxic fungicides, often killing pathogens in seconds. The goal of this study is to develop an environmentally friendly way to eradicate Cb in crop residue by utilizing hot water. Application of hot water is one of the most effective energy transfer methods and has the added benefit of not harming crop plants or the climate. The cost for tap water is less than a penny per gallon, compared to up to $600 per gallon for commercial fungicide. This study uses heat transferred via water to reduce Cb prevalence, as water infiltrates into crop residues and reaches target pathogens fast and effectively. Experiments were conducted in a greenhouse on dry and fresh crop residues. Each of those samples is placed on a 15-cm diameter plate and evaluated to optimize conditions of temperature at 50-90°C, volumes between 10-100 ml, and droplet size between 100-400 microns of hot water and monitored for fungal growth or death for 3-5 days. The result indicated that boiling water can effectively kill Cb on crop residues and can facilitate sustainable management of Cb inoculum levels in sugarbeet fields. The system is scaled up for outdoor applications by deploying a UGV equipped with an on-demand water heater to heat water instantaneously through a heat exchanger. The benefit extends to applications on other biotic stresses (e.g., pest and weeds) for sustainable management of pesticides and herbicides.
Evaluation of Cercospora leaf spot and post-harvest handling on sugarbeet storage.
HENDERSHOT, CARLY*1, CHRIS BLOOMINGDALE1, HOLLY CORDER1, TOM GOODWILL2, SARAH RUTH1, RANDY BEAUDRY1, LINDA E. HANSON2 and JAIME F. WILLBUR1, 1Michigan State University, Plant Biology Laboratory, 612 Wilson Road, Room 35, East Lansing, MI 48824, 2USDA-ARS, Plant Biology Laboratory, 612 Wilson Road, Room 37, East Lansing, MI 48824.
Abstract View Article
In Michigan, sugarbeets (Beta vulgaris) are stored for up to 200 days post-harvest, resulting in lower sugar yield due to rot and regular energy use from respiration. Cercospora leaf spot (CLS) has been considered a potential predisposing factor for increased storage rot and respiration. Over the past several years, Michigan studies of in-season effects of CLS on post-harvest rot susceptibility and North Dakota studies on respiration and other storage properties did not identify a significant interaction in hand-harvested beets. However, previous research and observations in commercial storage present conflicting evidence of leaf spot impacts on storability. To further investigate the impact of CLS on commercial storage, post-harvest symptom development was evaluated in bruised beets with relatively high or low in-season CLS severity. Bruised and non-bruised tissue of roots of each CLS level were inoculated with Fusarium graminearum, Botrytis cinerea, or Penicillium vulpinum and symptoms assessed after eight weeks. Over two years and six timepoints, CLS level did not significantly impact rot length, width, depth, and volume in bruised beets for the tested pathogens and varieties (P > 0.05). Despite numerically elevated electrolyte leakage, CLS did not significantly impact relative electrolyte leakage in the two years and four timepoints measured (P > 0.05). These results indicate CLS did not predispose beets to harvest damage or increased rot from the tested pathogens. Bruise significantly increased total rot volume at all six timepoints across crown and mid-beet impact sites (P < 0.05), and significantly increased rot depth at five timepoints, width at three timepoints, and length at two timepoints (P < 0.05). Commercial storage facilities continue to stress the apparent correlation between high CLS and poor storage. Research is continuing to attempt to elucidate the cause of these observations including the effects of CLS on beet physiology and other storage rot pathogens.
Screening root maggot lines/hybrids for genetic tolerance.
HANSEN, ADAM*, DAN BJUR and TYLER RING, United Beet Seeds, 1020 Sugar Mill Road, Longmont, CO 80501, USA.
Abstract View Article
Sugarbeet Root Maggots, Tetanops myopaeformis is an insect pest of Sugarbeet that has caused increased production challenges in several growing regions. The main areas of concern where growers have seen economic loss are Northeastern North Dakota, Northwestern Minnesota, Wyoming/Colorado, and Idaho. Root Maggot’s reduce plant stands, plant vigor and can cause a significant reduction of grower yield and profit. With uncertainty year to year on key insecticides being registered for use and the longevity that they will be available to use as a tool to control SBRM in the future has caused concern for growers. In addition to spraying insecticide and other common practices, growers are also reliant on Sugarbeet insecticide seed treatments to help with control. United Beet Seeds recognizes the importance and continued need for SBRM tolerant hybrids and is actively placing emphasis on our SBRM breeding program to address the growing concern of Root Maggots throughout the US. A combination of line development and line evaluation has taken place in the Red River Valley and is showing positive results for improved SBRM tolerance in United Beet Seed varieties. We plan to continue these research efforts to bring our growers the best hybrids possible for years to come.
Abiotic factors affecting accumulation of betaine in sugarbeet leaves and roots.
EIDE, JOHN D.*1, ABBAS LAFTA2, FERNANDO FINGER3 and KAREN K. FUGATE1, 1USDA, Agricultural Research Service, ETSARC, 1616 Albrecht Blvd. N., Fargo, ND 58102, 2North Dakota State University, Dept. of Plant Pathology, Fargo, ND 58108, 3Federal University of Viҫosa, Visҫosa, MG, Brazil.
Abstract View Article
Environmental stresses stimulate accumulation of osmoregulators in plants, including sugarbeet (Beta vulgaris L.). Betaine is a compound synthesized in response to abiotic stresses, including high soil saline content and drought. The application of growth regulators implicated in abiotic stress responses, methyl jasmonate (MeJA) and salicylic acid (SA), stimulate increased betaine levels in many plant species. Little information is currently available regarding the effects of abiotic stresses and SA and MeJA on sugarbeet betaine levels. Research, therefore, was conducted to determine betaine levels in sugarbeet seedlings and roots in response to abiotic stress and exogenously applied MeJA and SA. Drought stress significantly increased root betaine concentrations by 156% and 148% in roots of 16-week-old plants at harvest and after 12 weeks in storage, respectfully. In seedlings, betaine concentrations were elevated by 520% in response to drought and 321% in response to high saline conditions. When sugarbeet seedlings were treated with 0, 0.1, or 1 mM SA or 0, 0.1, or 10 µM MeJA, only the SA treated beets had significantly higher betaine than the untreated controls. Overall, these results demonstrate that sugarbeet betaine concentrations increase in roots and seedlings in response to drought, and in seedlings in response to salt stress and exogenous application of salicylic acid.
Sugar beet defense responses are significantly modulated during a susceptible interaction with Cercospora beticola.
EBINEZER, LEONARD BARNABAS*1,2, MARI NATWICK1,2, LORENA RANGEL1, MELVIN BOLTON1 and NATHAN WYATT1, 1USDA, Northern Crop Science Laboratory, 1307 18th Street North, Fargo, ND 58102, 2North Dakota State University, Department of Plant Pathology, Walster Hall 306, Fargo, ND 58102.
Abstract View Article
Sugar beet (Beta vulgaris L.) is the sole source of sucrose production in temperate regions worldwide and hence, is a very important economic crop. Among the many constraints to sugar beet cultivation, Cercospora leaf spot (CLS), caused by the fungal pathogen Cercospora beticola, is the most destructive foliar disease globally. To better understand the sugar beet – C. beticola interaction, it is pertinent to elucidate the sugar beet defense mechanisms that are modulated during infection. Herein, we present a time-course RNAseq analysis of a compatible (susceptible) interaction between sugar beet and C. beticola spanning the asymptomatic (3, 7 days post inoculation (DPI)) and symptomatic (11, 17, 25 DPI) phases of the infection process. We found that the defense response was the most altered functional category of differentially expressed genes with various classes of cell surface receptors and intracellular receptors mediating pathogen recognition being differentially expressed. We also observed differential expression of genes associated with reactive oxygen species generation, Ca2+ homeostasis, mitogen-activated protein kinase activation, callose deposition, stomatal movement, and defense associated transcription factors. A major proportion of the defense response associated genes that were upregulated at 3 DPI were repressed at 7 DPI but were strongly upregulated again during the symptomatic phase (11-25 DPI). Our results have provided preliminary indication that C. beticola could target and proficiently modulate these mechanisms to successfully evade, suppress or induce sugar beet defense ostensibly for its advantage. Taken together, our results have provided a transcriptomic snapshot of the sugar beet defense responses that were modulated during C. beticola infection.
Mapping-by-sequencing identifies loci linked to Rhizoctonia Root and Crown Rot resistance.
DORN, KEVIN M.*, NICHOLAS METZ, ANN FENWICK, AMY FALL and OLIVIA TODD, USDA-ARS, 1701 Centre Avenue, Fort Collins, CO 80526.
Abstract View Article
While significant breeding progress towards improved yield and agronomic traits in sugar beet, continued losses due to multiple pests and pathogens highlight the need for continuing efforts to improve host plant resistance. The crop wild relative sea beet (Beta vulgaris spp. maritima) has served as an essential source of novel disease resistance traits not present in the primary gene pool. Rhizoctonia Root and Crown Rot (RRCR), caused by the ubiquitous soil-borne fungal pathogen Rhizoctonia solani in sugar beet, regularly causes upwards of $50 million in actualized losses in the US annually. We have successfully mapped the genomic location of a novel quantitative trait loci (QTL) for RRCR resistance derived from a USDA-ARS Fort Collins pre-breeding germplasm called FC709-2 using bi-parental mapping in two beet populations. To continue searching for additional forms of RRCR resistance, we have extended this search into a sea beet mini-core collection. To enable the use of a modified bulk segregant analysis and sequencing approach to map resistance loci from this collection, we have developed the first fully-phased chromosome scale assembly of a RRCR resistant sea beet plant. Our results suggest the identified sea beet RRCR resistance is from this panel is genetically distinct from the FC709-2 sugar beet derived resistance. Work is ongoing to reconcile the genomic regions previously identified in previous QTL mapping studies, as well as to identify causal candidate gene(s) from the sea beet genome. The identification of these loci linked to RRCR resistance should enable the stacking of robust resistance to a globally important disease facing beet production, plus providing the genomic and germplasm resources necessary to better study the molecular interaction between Rhizoctonia and beet.
Genomic insights into the evolution, diversity, and trait discovery in cultivated beets (Genus Beta).
DHIMAN, AMAR S.*1, DEMETRIS TALIADOROS2, EVA H. STUKENBROCK3 and CHRISTIAN JUNG4, 1KWS SAAT SE & Co. KGaA, 37574, Einbeck, Germany, 2Department of Medical Biochemistry and Microbiology, Genetics and Genomics, Matthew Webster, Uppsala University, BMC, Husargatan 3, 752 37 UPPSALA, Sweden, 3Botanical Institute, Christian-Albrechts-University of Kiel, Am Botanischen Garten 1-9 24118 Kiel, Germany, 4Plant Breeding Institute, Christian-Albrechts-University of Kiel, Olshausenstr. 40, D-24098 Kiel, Germany.
Abstract View Article
The genus Beta encompasses important crops like sugar, table, fodder, and leaf beets. These cultivated beets are believed to have originated from wild progenitors of sea beet (Beta vulgaris subsp. maritima). Sugar beet has been selectively bred over the past 200 years to enhance root yield and sucrose content, making it economically significant. To investigate genetic diversity, a Beta diversity panel comprising wild and cultivated beet accessions was assembled. Whole-genome sequencing identified 10.3 million single nucleotide polymorphisms (SNPs) and revealed four genetic clusters: table beet, sugar beet, Mediterranean sea beet, and Atlantic sea beet. Phylogenetic analysis indicated that cultivated beets, especially those with storage roots, are genetically closer to Mediterranean sea beet, suggesting a shared ancestor. Regions with reduced nucleotide diversity in cultivated beets highlighted areas of selection during domestication. Candidate genes in these regions are linked to key traits such as root development, flowering time regulation, and sucrose metabolism. A novel sucrose transporter on chromosome 6 exhibited reduced nucleotide diversity in sugar beet, indicating its role in sucrose storage. Further analysis revealed high nucleotide diversity among accessions with contrasting root phenotypes, highlighting two genes encoding auxin response factors critical for root development and thickening. Genome-wide association studies (GWAS) identified marker-trait associations for nine phenotypic traits, clarifying the genetic control of important characteristics. Notably, the identification of the M locus controlling monogermity—a desirable trait that facilitates efficient planting—was a major finding. The strongest association for monogermity was found at the end of chromosome 4, marking a breakthrough for developing uniform monogerm sugar beet varieties. The study also investigated the genetic basis for pigmentation in table and leaf beets. Accumulation of betalain pigments, responsible for red and yellow colors, was associated with regions near two key genes: Cytochrome P450 and a MYB-like transcription factor, both vital for betalain biosynthesis. Moreover, significant genetic associations for resistance to Cercospora leaf spot, a damaging fungal disease in beets, were identified on chromosomes 1, 2, 7, and 9, corresponding to genes encoding leucine-rich repeat (LRR) and nucleotide-binding site (NBS) proteins involved in plant defense. This analysis of genetic and phenotypic diversity within Beta clarifies the evolutionary history and domestication of these crops and provides valuable genetic resources for breeding programs aimed at enhancing sucrose content, root development, disease resistance, and pigmentation. The identification of key genes related to traits like monogermity and betalain biosynthesis marks significant advances in understanding beet genetics, benefiting both commercial cultivation and scientific research.
Exploring genetic structure and diversity of breeding material producing three-way hybrids in sugar beet: impacts on male-female differentiation, and genome-wide patterns.
DESPREZ, AUGUSTIN*1,2, MAUD TENAILLON2, KARINE HENRY1, ALAIN CHARCOSSET2, BRUNO DESPREZ1, PIERRE DEVAUX1 and LAURENCE MOREAU2, 1Florimond Desprez Veuve & Fils SAS, BP 41, 59242, Cappelle-en-Pevele, France, 2INRAE, CNRS, AgroParisTech, GQE-Le Moulon, Université Paris-Saclay, 91190, Gif-Sur-Yvette, France.
Abstract View Article
Since F.V. Owens’ discovery of cytoplasmic male sterility (CMS) in 1945, and G.J. Hogaboam first hybrids in 1957, to manage both heterosis and fixation of alleles, a lot of progress have been done, resulting into male and female pool structuration. Sugar beet hybrids are also characterized by numerous successive introductions of specific and important traits such as monogermity from SLC101 (Savitsky, 1950), or rhizomania tolerance/resistance from Holly sugar germplasm, wild beets WB42 and WB41… These traits’ introductions using more or less distant donors disrupted the structuring of male and female pools, with often loss in genetic values. Identifying the key aspects of the resulting evolution of structuration, and the impacts of these successive introductions is a first step in developing more efficient breeding and pre-breeding methods. Genotyping data are now available and can be used to study diversity within the different breeding programs, both globally and across the genome, and its evolution. Linking these findings with phenotypic data will also help understand the impact of breeding methods. This will enable the development of new efficient strategies for a sustainable response to environmental challenges through optimized breeding approaches for both short- and long-term efficiency in diversity management. This study is then focusing on genetic diversity and its genome distribution in a sugar beet breeding context with a wide panel of 30 thousand plants (parental components and resulting hybrids) covered by 10k biallelic SNPs. One may identify genomic regions that exhibit patterns in response to differentiation between populations, past introductions of traits or genetic drift. Results demonstrate how breeding design is structuring pools globally, and how local regions are impacted. This can provide opposite patterns depending on the breeding methods involved. Using Fst (Weir & Cockerham, 1984) between male and female accessions, as well as diversity indexes, highlight shifts in allelic frequencies in those two pools linked with breeding.
Rotating DMI fungicides within spray programs to manage Cercospora beticola in sugar beet.
DELEON, JAMES A.1, AUSTIN K. LIEN1,2 and ASHOK K. CHANDA*1,2, 1University of Minnesota Northwest Research & Outreach Center, 2900 University Avenue, Crookston, MN, 56716, 2University of Minnesota, Dept. of Plant Pathology, 1991 Upper Buford Circle, St. Paul, MN, 55108.
Abstract View Article
Cercospora leaf spot (CLS), a devastating foliar disease of sugarbeet, is caused by the fungal pathogen Cercospora beticola and is primarily managed by use of fungicides. Demethylation inhibitor (DMI) fungicides are typically applied two to three times in a growing season as a tank-mix with a broad-spectrum contact fungicide. In recent years, C. beticola isolates have developed patterns of cross-resistance to select DMI fungicides. In 2023 and 2024, field trials were conducted to evaluate the efficacy of 4-spray programs with two alternating DMI fungicides, beginning with either tetraconazole (TET) or mefentrifluconazole (MEF) in the first application and rotating with either a pre-mix of difenoconazole (DIF) + propiconazole or prothioconazole (PRO) in the third application. Weekly CLS severity ratings were used to calculate the standardized area under the disease progress stairs (sAUDPS). Additionally, 5 leaves were collected from each plot at the end of the season to assess CLS severity and obtain multiple single-conidia isolates. The effective concentration of fungicide that inhibits 50% of growth compared to the control (EC50) was determined for TET, MEF, DIF, and PRO using a microplate-based assay. A multiplex probe-based qPCR assay was used to distinguish between five potential haplotypes (A-E) of CbCyp51, which encodes the DMI target enzyme. CLS severity was significantly lower in all fungicide treatments compared to the nontreated control. Despite known cross-resistance among TET and PRO, and MEF and DIF, these fungicides effectively managed CLS in spray program that utilized multiple mode-of-action fungicides as tank-mix partners. In 2023, haplotype E was predominant among the isolates which is known to be associated with decreased sensitivity to all four DMI fungicides. Overall, the evaluation of DMI fungicide spray programs and their impact on the development of fungicide resistance will help maintain their continued effectiveness and minimize economic losses in sugarbeet.
SBR and RTD - A new threat challenging breeding and agronomy in sugarbeet cultivation in Europe.
CZARNECKI, OLAF, HENNING EBMEYER* and MARIO SCHUMANN, KWS SAAT SE & Co. KGaA, Grimsehlstrasse 31, 37574 Einbeck.
Abstract View Article
Two new sugarbeet diseases, “Syndrome des Basses Richesses” (SBR) and “Rubbery Taproot Disease” (RTD), are spreading in certain regions of Southern and Eastern Germany, Switzerland as well as South-East European countries. Both diseases are transmitted by plant hoppers (e.g. Pentastiridius leporinus, Reptalus quinquecostatus or Hyalesthes obsoletus), whose occurrence varies across Europe. They serve as vectors for two bacterial pathogens, the proteobacterium Candidatus Arsenophonus phytopathogenicus and/or the phytoplasma Candidatus Phytoplasma solani. Currently, it is assumed that the proteobacterium is the main causal agent of SBR, while RTD-expressing sugarbeet are infected with phytoplasma. SBR-infected sugar beet plants display severe symptoms including leaf yellowing and necrosis, asymmetric growth of lancet-shaped leaves, brown discoloration of taproot tissue, but most importantly, a loss of sugar content of up to 5% (absolute) and yield losses of up to 25%. RTD-infected sugarbeet suffer from severe water loss at very late stages of plant development, resulting in rubbery tissue that affects storage and processing. Severe yield reductions and susceptibility to secondary fungal and bacterial infections are the result. Therefore, the diseases must be brought under control by sugar beet breeders introducing genetic resistance and developing SBR- and RTD-tolerant or resistant varieties. Breeding programs are being established that also include the screening of wild beet accessions as genetic resources for SBR and RTD tolerance. A large-scale monitoring program is in place to monitor the spread and composition of pathogens and the flight activity of planthoppers. In addition, a rearing of planthoppers is being established to enable year-round experiments under controlled conditions. The agronomic approaches include investigation of the timepoint of planthopper immigration by controlled planthopper immigration with the use of net cover over the trial plots. Further investigations will evaluate the potential of seed treatment to control the planthoppers in the nymphal stage within the winter wheat crop rotation.
Development of a high-throughput computational pipeline to identify new chemical management strategies for sugar beet pathogens.
CHAPPIE, JOSHUA S.*1, NATHAN A. WYATT2 and SAMUEL WYATT2, 1USDA-ARS Soil Management and Sugarbeet Research Unit, Crops Research Laboratory, 1701 Centre Ave, Fort Collins, CO 80526, 2USDA-ARS Edward T. Schafer Agricultural Research Center, Sugarbeet Research Unit, 1616 Albrecht Blvd. North, Fargo, ND 58102.
Abstract View Article
Fungal and viral diseases cause significant economic losses to U.S. sugar beet farmers. Despite extensive efforts to breed more resilient sugar beet varieties, pathogens like Cercospora beticola, Rhizoctonia solani, Fusarium oxysporum, and beet curly top virus remain problematic, owing in part to evolutionary shifts in virulent populations as a response to the selective pressures imposed by different agronomic practices (e.g., application of fungicides). The persistence of these harmful agents underscores the need for new management strategies. Here we describe a computational pipeline that leverages advances in machine learning, protein structure modeling, and high-throughput drug discovery to identify compounds that can reduce fungal and viral infections in a field setting. This framework implements small molecule docking protocols to screen diverse chemical libraries in silico, specifically targeting the molecular interactions and functional activities that contribute to pathogenesis and disease progression. Our approach is highly adaptable to different biological contexts and complementary to existing defense mechanisms within a host plant. By this methodology, we found several compounds that may function as putative inhibitors of secreted Cercospora effector proteins. Experiments to validate the specificity of these chemicals and characterize their effectiveness in combatting Cercospora leaf spot disease are ongoing.
Impact of fungicide application timing on Cercospora beticola latent infection, symptom development, and fungicide resistance profiles during the growing season.
BRANCH, ERIC A.*1, ANDREW W. FUCHS1, SOPHIA TRUSCOTT2 and NATHAN A. WYATT2, 1North Dakota State University and University of Minnesota, NDSU, Dept. 7660, PO Box 6050, Fargo, ND 58108-6050, 2USDA-ARS, Edward T. Schafer Agricultural Research Center, Sugarbeet Research Unit, Fargo, ND 58102
Abstract View Article
This project assessed performance of spray programs with early first applications and generated preliminary data on the relationship between different fungicide programs, latent Cercospora beticola infections, and the fungicide resistance profile of the C. beticola population. In North Dakota and Minnesota, Cercospora leaf spot (CLS) caused by the fungus C. beticola, continues to limit sugarbeet yields and economic returns for growers and cooperatives. Growers rely on fungicide applications made at or around sugarbeet row closure and continued throughout the season. Two small-plot replicated field trials were conducted in the 2024 season near Foxhome, MN. Ten treatments, including a nontreated control, were tested on both a CR+ and non-CR+ sugarbeet variety. Treatments differed in timing of the initial fungicide application (12-Jun, 28-Jun, Jul-12, or 29-Jul), and the interval between subsequent applications (based on calendar days, CLS disease infection values, or a combination). Fungicide products and active ingredient rotation were the same between treatments once applications began. Initiating the fungicide program beginning on 12-Jun offered no statistically significant benefit compared to a 28-Jun start, but did provide numeric improvements in CLS ratings and recoverable sucrose per ton. Prior to the start of fungicide applications, leaf samples were collected from each plot, and again prior to each subsequent application in the program. Analysis of C. beticola DNA extracted from leaf tissue showed changes in the relative abundance of strobilurin (G143A), benzimidazole (E198A), and triazole (E170, L144F) fungicide resistance alleles over time as the growing season progressed.
Exploration of secondary metabolites as biocontrol agents against sugar beet disease.
BACK, DANIEL C.*1, MADISON CHRISTENSON2, MARI NATWICK2 and MELVIN D. BOLTON1, 1USDA-ARS, Edward T. Schafer Agricultural Research Center, Sugarbeet Research Unit, Fargo, ND 58102, 2North Dakota State University, Fargo, ND, 58102.
Abstract View Article
Sugar beet pathogens are a major issue that greatly impact the yield of sugar beets harvested each year. Developing new strategies to combat sugar beet disease is vital for the growth and survival of the sugar beet industry. The sugar beet plant is home to its own microbial community that relies on the health of the sugar beet plant to ensure its survival. Therefore, certain sugar beet endemic microorganisms have developed their own strategies to defend themselves against sugar beet diseases. The aim of this study is to identify small molecule secondary metabolites from the sugar beet microbiome that aid in the defense against sugar beet pathogens. In this study we have identified a variety of secondary metabolites from Burkholderia contaminans, a bacterial species isolated from sugar beet leaves shown to inhibit the growth of the sugar beet pathogen Cercospora beticola. Additionally, the identification and biosynthesis of metabolites from C. beticola is a key aim of this study to better understand the strategies used by C. beticola to infect the sugar beet plant. Finally, explorations of metabolites from an entomopathogenic fungus isolated from sugar beet root maggot is underway to gain insight into strategies used to combat this pest.
Development of a rapid seedling assay for evaluating sugar beet Fusarium tolerance.
ANFINRUD, MARK*1, VIVIANA RIVERA2, GARY A. SECOR2, MELVIN D. BOLTON1, CHENGGEN CHU1 and NATHAN A. WYATT1, 1USDA-ARS, Edward T. Schafer Agricultural Research Center, Sugarbeet Research Unit, Fargo, ND 58102, 2North Dakota State University Department of Plant Pathology, Fargo, ND 58102.
Abstract View Article
Fusarium species, such as F. oxysporum and F. secorum, are damaging soil borne pathogens of sugar beet that cause root rot and foliar damage. Management of Fusarium diseases is heavily reliant on the use of resistant sugar beet cultivars as no fungicide management has been established for Fusarium pathogens. Breeding for tolerance to Fusarium species is laborious due to the lack of rapid assays to screen available germplasm for Fusarium tolerance. Here we developed a sugar beet seedling assay that distinguishes between Fusarium tolerant and susceptible sugar beet varieties. The assay is performed using sterile pleated paper inoculated with Fusarium conidia that were incubated for seven days at 25℃. Significant differences in disease scores were observed between established tolerant sugar beet seedlings and susceptible seedlings. This new assay will be a valuable tool both for supplementing traditional field trials for Fusarium tolerance and as a rapid assay to screen germplasm for Fusarium tolerance.
Processing - Oral
Functional equivalent of a direct discharge - Do discharges to ground water at your facility require an NPDES Permit?
SINNER, KATE*, Barr Engineering Co., 325 South Lake Avenue, Suite 700, Duluth, MN 55802.
Abstract View Article
The April 2020 Supreme Court of the United States (SCOTUS) decision in County of Maui v. Hawaii Wildlife Fund, 140 S. Ct. 1462 (Maui Decision) held that a National Pollutant Discharge Elimination System (NPDES) permit is required “when there is a direct discharge from a point source into navigable waters or when there is the functional equivalent of a direct discharge.” Specifically, the Maui decision determined that underground injection wells at a wastewater treatment plant on the Island of Maui, Hawaii, which discharged wastewater to groundwater that travels to the Pacific Ocean, needed an NPDES permit because the discharge is the functional equivalent of a direct discharge of pollutants to a water of the United States (WOTUS) (Pacific Ocean). In November 2023, the EPA released draft guidance to provide additional permitting considerations and direction for the implementation of the Maui decision in the NPDES permit program. The EPA’s draft guidance applies to point source discharges that reach a WOTUS via groundwater or other subsurface flow. EPA held a comment period for the draft guidance in November and December 2023. EPA’s next action to publish final guidance is expected at any time. Prior to the Maui decision, case law was divided on whether the Clean Water Act governs discharges into groundwater that is hydrologically connected to a WOTUS. The Maui decision was the first decision at the SCOTUS level to clarify that the scope of the NPDES permitting program includes some discharges through groundwater to surface waters. The Maui decision and forthcoming final guidance document will affect facilities that have potential functional equivalent discharges. Sugar beet facilities with discharges to groundwater from features such as unlined ponds, spray fields, or land application sites may need to conduct functional equivalent evaluations. This presentation will provide a brief regulatory background behind the Maui Decision, discuss the draft guidance (or final guidance, if this is released by the time of the presentation), potential implications to sugar beet facilities, and NPDES permitting precedence to date for the functional equivalent issue.
Measurement and mitigation strategies for elevated viscosities resulting from microbes present in sugar beet processing.
TERRELL, EVAN*1 and GILLIAN O. BRUNI, 1USDA, Agricultural Research Service, Southern Regional Research Center, 1100 Allen Toussaint Blvd., New Orleans, LA 70124.
Abstract View Article
Microbes are introduced into processing streams from infected beets, piles, and soils, creating operational challenges and overall sugar losses. Contaminating bacteria consume sucrose while producing exopolysaccharides (EPS), like dextrans and fructans, resulting in potentially increased viscosities and associated difficulties with sugar recovery and crystallization. In this study, a set of ~50 bacterial strains from juices and biofilms representing some common genera among isolates were grown in high sucrose-containing medium. Culture broth viscosities were measured using a Brookfield model DV-II+ viscometer with a UL spindle and small sample adapter. A smaller set of samples were grown in the presence of (A) an oxidizing antimicrobial agent, (B) a dextranase enzyme, and (C) an antimicrobial and dextranase concurrently. These samples were measured for viscosity and optical density (OD). In total, 37 flask cultures were classified as watery, 7 flask cultures were classified as intermediate, and 10 flask cultures were classified as highly viscous. The juice and biofilm isolate culture samples had similar absolute numbers of watery and intermediate class samples (juice, watery: n=18; juice, int.: n=4; biofilm, watery: n=19; biofilm, int.: n=3). However, the biofilm-derived isolates had roughly double the number of viscous cultures compared to juice (juice, viscous: n=3; biofilm, viscous: n=7). These results seem consistent with observations that that EPS from biofilm isolates may result in higher viscosities than EPS from juice (planktonic) isolates. Finally, there seemed to be a correlation between increased viscosity and observations of mucoid colony morphology on beet juice agar during the microbial isolations. For experiments on the effect of processing aids, results show that untreated controls of bacterial cultures had high culture viscosity and OD, with treated bacterial cultures being lower. Application of dextranase as a treatment alone resulted in somewhat elevated OD and viscosity values for resulting culture solutions. The antimicrobial treatment was more effective at inhibiting microbial growth; however, the lowest OD and viscosities resulted from the concurrent application of both enzyme and biocide together. This provides evidence to support the hypothesis that EPS mitigation strategies may introduce synergistic microbial control when antimicrobial agents are applied. The results show that there is a wide variety among processing bacteria for propensity to form EPS and elevated viscosities. Through the application of treatments like dextranases, bacteria present during processing may have less opportunity to form EPS and biofilms. As a result, this could predispose dextran-forming bacteria to control measures, like the application of antimicrobial agents. Overall, this strategy may improve operational efficiencies by decreasing required volumes/costs for processing aids and increasing sugar production.
Minimizing the carbon footprint of the Billings factory.
ZIMMERMAN, ROBERT*1, LUKE P. MCLAUGHLIN2, JEREMY SMENT2, JEFFREY GIFFORD3, BRANDON KRUSE4, 1The Western Sugar Cooperative, 1221 8th Ave, Suite E, Greeley, CO 80631, 2Sandia National Laboratories, 1515 Eubank Blvd SE, Albuquerque, NM 87123, 3National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401, 4Magaldi Technologies, LLC, 30000 Milcreek Avenue, Suite 385, Alpharetta, GA 30022.
Abstract View Article
The Billings Factory is the only remaining Western Sugar Cooperative (WSC) factory with coal boilers. WSC is looking at technologies to replace these boilers with reduced carbon emissions technologies. These include higher pressure natural gas boilers with steam turbine electrical generation capability for the base load steam production. This will be supplemented with photovoltaic electrical (PV) generation coupled with a thermal battery to allow for steam and electrical generation 24 hours/day. The PV system would be used to generate electricity year-round with surplus being sold to the grid when the factory is not operating. The thermal battery would allow for dispatching electrical energy from PV during peak usage utilizing the steam turbine generator set. In combination with steam conservation projects, the overall carbon footprint will be reduced by 50-75% at the completion of this project. The PV/Thermal Battery system is modular so further reductions in carbon emissions are available with additional spending. A Phase I study partnered with the National Renewable Energy Laboratory, Sandia National Laboratories and Magaldi Technologies, LLC is underway. This paper will discuss the technology and findings of this study.
Functional equivalent of a direct discharge - Do discharges to ground water at your facility require an NPDES Permit?
SINNER, KATE*, Barr Engineering Co., 325 South Lake Avenue, Suite 700, Duluth, MN 55802.
Abstract View Article
The April 2020 Supreme Court of the United States (SCOTUS) decision in County of Maui v. Hawaii Wildlife Fund, 140 S. Ct. 1462 (Maui Decision) held that a National Pollutant Discharge Elimination System (NPDES) permit is required “when there is a direct discharge from a point source into navigable waters or when there is the functional equivalent of a direct discharge.” Specifically, the Maui decision determined that underground injection wells at a wastewater treatment plant on the Island of Maui, Hawaii, which discharged wastewater to groundwater that travels to the Pacific Ocean, needed an NPDES permit because the discharge is the functional equivalent of a direct discharge of pollutants to a water of the United States (WOTUS) (Pacific Ocean). In November 2023, the EPA released draft guidance to provide additional permitting considerations and direction for the implementation of the Maui decision in the NPDES permit program. The EPA’s draft guidance applies to point source discharges that reach a WOTUS via groundwater or other subsurface flow. EPA held a comment period for the draft guidance in November and December 2023. EPA’s next action to publish final guidance is expected at any time. Prior to the Maui decision, case law was divided on whether the Clean Water Act governs discharges into groundwater that is hydrologically connected to a WOTUS. The Maui decision was the first decision at the SCOTUS level to clarify that the scope of the NPDES permitting program includes some discharges through groundwater to surface waters. The Maui decision and forthcoming final guidance document will affect facilities that have potential functional equivalent discharges. Sugar beet facilities with discharges to groundwater from features such as unlined ponds, spray fields, or land application sites may need to conduct functional equivalent evaluations. This presentation will provide a brief regulatory background behind the Maui Decision, discuss the draft guidance (or final guidance, if this is released by the time of the presentation), potential implications to sugar beet facilities, and NPDES permitting precedence to date for the functional equivalent issue.
Reduction in Energy consumption by maximizing the dry substance of the beet pulp leaving your Beet pulp press – Long term case history from Süd Zucker.
SCHOFIELD, PHILIP and MARKUS WEINGARTNER, ANDRITZ Fiedler, Weidener Str. 9, 93057 Regensburg, Germany.
Abstract View Article
Sugar beet mills who dry their pulp in order to pelletize, can save significant energy consumption by increasing the dry substance of the pulp during the pressing stage of the process. This can be achieved by using maximum open area, duplex material, drilled screens with a developed profile specific to this application along with optimum gap between screw flight tip and the screens. An eight year’s study of Beet Press performance at Sud Zucker demonstrates the benefits. ANDRITZ Designs, Manufactures and installs drilled screens and provides maintenance of screw flights.
Experiences with the installation of new gas boiler and turbine/generator at Western Sugar Cooperative, Scottsbluff location.
RUGRODEN, PAUL R.*, Western Sugar Cooperative, 7555 East Hampden Avenue Suite 520 Denver, CO 80231.
Abstract View Article
Western Sugar Cooperative in Scottsbluff, NE recently installed two new Babcock and Wilcox shop assembled package natural gas boilers. The boilers are a model FM120-136 water tube design capable of providing 225,000 pounds per hour each of 6000F steam at 400 psig. The reasons for the installation include: unreliable and aged coal boilers, environmental regulatory requirements, and lessening of personnel load. Howden MONO AFA 6 single blade turbine coupled with a 7MW TDPS generator was installed and commissioned the following year. Westinghouse 5MW AC turbine generator had failed two years prior. This paper aims to provide lessons learned for future factory boiler conversions and installations.
Sustainability and energy efficiency in United States beet sugar processing.
RING, TANNER K.*, EAPC Industrial Services, 3100 Demers Ave, Grand Forks, ND 58201.
Abstract View Article
As legislation and shifting energy markets reshape the United States business landscape, sustainable business practices, carbon reduction, and highly energy-efficient operations are becoming increasingly crucial across all industries. No current federal legislation has established a cap-and-trade system or similar pricing for carbon emissions in the United States. Despite this, 48 states and the District of Columbia have developed State Climate Action Plans and/or Priority Climate Action Plans. Additionally, 23 states, along with the District of Columbia and Puerto Rico, have set specific greenhouse gas reduction goals. The United States beet sugar industry must be prepared to adapt to potential state and federal mandates on emissions reductions and carbon pricing. The European Union and Canada, with their mature tax and secondary market systems for carbon pricing, serve as models for the potential future. These systems have significantly influenced both their regional and global markets and promoted a shift towards sustainable energy solutions. They have also created new opportunities for alternative revenue streams from renewable energy production which could benefit from substantial tax credits and price incentives. To take advantage of these opportunities, increase energy efficiency, and prepare for market changes due to emissions pricing, the U.S. beet sugar industry must embrace a combination of traditional energy efficiency methods, modern sustainability practices, and innovative technologies. Projects that offer a positive return on investment with minimal to no reliance on tax credits or other incentives, cutting-edge technologies aimed at complete decarbonization and sustainable energy production, and every solution in between should be concurrently investigated and made ready to be implemented at the plant and company level to ensure the industry is prepared for all potential futures.
Improved energy usage of continuous crystallisation with vapour compression.
POPOV, WJATSCHESLAW*1, NILS TRÄBER1, DEIKE LAUE1 and ANDREAS LEHNBERGER1, 1BMA Braunschweigische Maschinenbauanstalt GmbH, Am Alten Bahnhof 5, 38122 Braunschweig, Germany.
Abstract View Article
By combining continuous crystallisation with mechanical vapour compression, the energy requirement can be significantly reduced. In crystallisation, the temperature difference between the heating steam and the vapour has to be kept low, which makes the vertical continuous pan VKT (“Verdampfungskristallisationsturm”) with minimum energy input particularly suitable for this process. Using custom strategies for a VKT with a plant configuration that comprises mechanical vapour compression, the energy requirement in the sugar house can be further reduced. This paper discusses the effect on the overall energy requirement of a beet sugar factory.
Membrane-based no-waste extract decolorization process.
PEACOCK, STEVE*1 and CY GAUDET1, 1Amalgamated Research LLC, 2531 Orchard Drive East, Twin Falls, Idaho 83301, USA.
Abstract View Article
Molasses desugarization using simulated moving bed chromatography has been widely adopted within the US beet sugar industry. Processing of the high-purity extract fraction from this operation can be integrated into the factory in a number of ways. However, since the extract is typically higher in both color and purity than thick juice, extract crystallization using the standard three-boiling scheme can be challenging. Adding the extract into the white boiling allows the extract to be more easily exhausted down to an acceptable final molasses purity through all three stages of crystallization but yields a higher product sugar color. Alternatively, adding the extract into the high raw boiling allows for more effective color elimination, but raises the purity of the final molasses due to the difficulty of exhausting such high purity material in only two crystallization stages. Ion exchange decolorization of the extract prior to crystallization is an attractive solution to this problem but would normally result in the generation of unacceptably large quantities of brine waste for disposal due to the high color loads involved. Recently, this problem was solved when Amalgamated Research developed and patented a “no-waste” decolorization process that uses membrane nano-filtration to recover inorganic salts from the separator raffinate stream and uses these to regenerate an ion exchange resin in extract decolorization service. After regeneration, the spent regenerant is recombined with the raffinate stream without affecting its value as a coproduct. Extensive pilot testing with materials from several facilities has demonstrated that high resin color loadings can be achieved, while providing extract decolorization of more than 70%. This promising technology offers the possibility of improving overall sugar-end performance by reducing sugar recycle, thereby increasing overall sucrose extraction.
Split & dual continuous pans – Design aspects for maximizing the benefit to sugar mills.
PANDURANGA RAO MEESALA*, SHRIJEE PROCESS ENGINEERING WORKS LTD, A 504 Dynasty Business Park, Andheri – Kurla Road, Andheri East, Mumbai 400059. India.
Abstract View Article
Indian and overseas sugar mills are expanding capacity and looking for innovative designs of various process house equipment which could minimize, steam consumption, power consumption, process losses, man power, required space for installation and better capacity utilization. To increase the revenue, sugar mills have to install the energy saving equipment in the process house to reduce steam & power consumption in addition to the improvement in the product quality. Split/Dual Continuous vacuum pans provides significant advantages such as complete automation, minimal supervision, steam economy, better exhaustion, uniform crystals, high rate of crystallization, high throughput, improved quality and maximum capacity utilization. The design concept of Split /Dual continuous pans has been developed by “M/S Shrijee Process Engineering Works Ltd”, Mumbai, India, to ensure the above benefits to the sugar mills. These pans can run with V3/V4 vapor from multiple effect evaporators. The split continuous vacuum pan is having the flexibility to operate at 50% to 100% of the designed capacity in correlation with beet/cane processing; and either side 50% of the pan can be taken for water boiling without stopping the complete pan. Dual continuous pan can be utilized for simultaneous boiling of any two grades of massecuite or any one grade of massecuite boiling or one massecuite boiling can be stopped and other massecuite can be boiled as and when required. Both, Split and Dual continuous pans, are having the provision to utilize the different pressure vapor simultaneously at different sides of the calendria. These pans have been running successfully for A, B and C massecuite in India and overseas. Shrijee Split continuous pans of each 180 m3 (6356.64 ft3) volume for “C” massecuite, “B” massecuite and “A” massecuite have been working with excellent results since 2018, 2020 and 2023 respectively in LASUCA, USA. A Split/Dual continuous vacuum pan provides major advantages to achieve more effective use of installed volumetric capacity than batch pans. Additional advantages are reduced supervision, lesser steam consumption, consistency in the product massecuite and easy operation. In order to maximize the benefits, Split/Dual continuous pans must be operated to achieve high rate of crystallization, maximum exhaustion and high throughput so as to get shorter return on investment. This paper shall emphasize several important designs and operating criterion for maximizing the benefits to the sugar mill from Split/Dual continuous pans.
Pond odor control 101: Practical solutions for retention pond odor management in sugar beet processing.
NELSON, MICHAEL L.*1, BRENDA E. BLAKE*1, BRADY G. OVERTON1, 1USP Technologies, 5640 Cox Rd, Glen Allen, VA 23060.
Abstract View Article
Odor control in retention ponds, especially those used in high-loading industrial processes like sugar beet processing, is essential for protecting environmental quality, maintaining positive community relations, and in some cases, meeting permit requirements. These odors originate from high biological oxygen demand (BOD) and chemical oxygen demand (COD) loadings, which deplete oxygen in the pond and lead to anaerobic conditions. This process results in the release of volatile, odorous compounds such as hydrogen sulfide (primarily), ammonia, amines, mercaptans, and organic acids, which can be a significant nuisance if left unaddressed. This presentation takes a wholistic approach to managing pond odors, starting with looking at how to keep sugar hits from happening in the first place (process improvements & high-risk events inside the process.) We will also go over best sampling and monitoring techniques to help identify and quantify the components causing nuisance odors. Using these methods also help to evaluate each treatment strategies’ efficacy. USP’s recommended approach involves monitoring parameters like COD/BOD, pH, temperature, dissolved oxygen, and key odorant compounds, along with specialized procedures, such as the Purge Test, to help assess volatile odorants in liquid samples. Additionally, we will cover the use of handheld and temporary/permanent installation for fence line and point source odor sniffing. With a clear assessment of pond conditions, targeted odor control strategies can be implemented to prevent volatilization and directly address pond odors. Strategies for reducing odorous compound volatilization include the use of binding agents, pH adjustments, and physical containment measures like controlled aeration timing or pond covers. Addressing pond septicity is equally important and may require aeration and oxygenation solutions, ranging from mechanical aeration to the addition of hydrogen peroxide, nitrates, or biocides to support biological balance. Controlling the pond’s microbial environment is another strategy for odor management. Through bioaugmentation, specific strains can be encouraged to proliferate and out-compete odor-producing bacteria. Additionally, liquid-phase oxidation treatments—whether through pond capping, targeted area applications, or full-volume treatment—can provide efficient odor reduction and an operational lever to pull during process upsets. For vapor-phase emissions, options like masking agents, cover-and-treat methods, and improved ventilation help to manage off-gassing effectively. These targeted approaches provide sustainable, reliable odor management solutions for retention ponds, supporting both sugar beet processing operations and the broader community by mitigating nuisance odors. This presentation will cover a wide variety of topics and will use the experience and expertise of multiple operators and vendors.
Experiences from automated centrifuge wash water control.
MUIR, BARBARA M1 and BJARNE C. NIELSEN*2, 1Tereos Corporate Technology Centre, Burchstraat 10, Aalst 9300, Belgium, 2Neltec Denmark A/S, Vestergade 35, 6500 Vojens, Denmark.
Abstract View Article
The agricultural raw material is received with significant variations. During processing some of these variations can be reduced, but other variation may be introduced by the process. After crystallization the separation in centrifuges gives an opportunity to adjust the final sugar color by varying the amount of wash water to remove more or less of the crystals’ surface film of mother liquor. Due to rapid changes in the massecuite, it is customary to wash more than strictly necessary, so crystals with color outside specifications can be avoided with high probability. Factories check the quality by samples taken at 1 hour, 2 hours, or larger intervals, either as snap or composite samples. The amount of wash is adjusted accordingly, but always leaving a comfortable safety margin to the color limit. Real-time colorimeters mounted so they can measure the crystal color just after wash eliminate the risk of variations between samples and ensure the measurements are representative, as all sugar is measured (except for sugar covered by discharges from other centrifugals). Such instruments have been installed in factories for real-time adjustment of the wash. Result about reduced water usage, reduced dissolution of sucrose, and other process improvements will be given. Keywords: sugar color, centrifuge wash water, automation, real-time measurement
Quantitative analysis of sucrose, raffinose, and moisture content in sugar beets utilizing hyperspectral imaging and advanced machine learning techniques.
MOJOKO, IVANNA1, NALOVA LISINGE*1, BILLY G. RAM2, EWUMBUA MONONO2, ZHAO JIN3, XIN SUN2 and SHYAM L. KANDEL4, 1North Dakota State University, School of Natural Resources Sciences, Fargo, ND 58108, 2North Dakota State University, Department of Agricultural and Biosystems Engineering, Fargo, ND 58108, 3North Dakota State University, Department of Plant Sciences, Fargo, ND 58102, 4USDA-ARS, Edward T. Schafer Agricultural Research Center, Fargo, ND, 58102.
Abstract View Article
Sugar beets account for 20-25 percent of the global sugar demand. However, post-harvest losses due to sucrose degradation can significantly impact on the overall yield and quality of sugar. Accurate and timely monitoring of sucrose content is crucial to minimize these losses. Traditional laboratory methods, while precise, are often time-consuming, labor-intensive, and destructive. Hyperspectral imaging emerges as a promising alternative, offering rapid, non-destructive, and accurate analysis of various quality parameters, including sucrose content. To develop robust hyperspectral imaging model for sugar beet quality assessment, sugar beet samples will be randomly collected from three locations: Moorhead and Renville in Minnesota, and Wahpeton, North Dakota. Sugar beet roots will be washed, sliced into 15 mm thickness, and spectral images will be acquired using the Specim SWIR camera in the spectral range of 1,000-2,500 nm. Images will first undergo radiometric correction to remove noise and mitigate any illumination variations. Pre-processing methods, such as Standard Normal Variate for normalization and Savitzky-Golay filtering for derivative-based signal smoothing, will be applied. For feature extraction, discriminant analysis and Principal Component Analysis will be employed. The 10 most significant features, representing sucrose, raffinose, and moisture content, will be selected from the raw 224 spectral signatures. The extracted features and pre-processed signals obtained from standard procedures will be combined into a single dataset. This dataset will then be split into training, validation, and testing sets in a 6:2:2 ratio. Various machine learning and deep learning algorithms will be used for classification. It is anticipated that Linear Discriminant Analysis and Artificial Neural Networks will perform best, with high correlations of 0.90 and 0.95 and standard errors of prediction of 0.51 and 0.56. These results are expected to support the claim that machine learning models trained on spectral signatures cannot destructively predict sucrose, raffinose, and moisture content in sugar beets. Keywords: Hyperspectral imaging, Sucrose, Machine learning, Deep learning.
Assessment of sucrose solubility in American Crystal Sugar Company’s Technical Sucrose Solutions.
KOBRINSKY, HUDI S.*, NICK MORITZ, DAVID R GROOM, and BILL MCKEWAN, American Crystal Sugar Company, 1700 North 11th Street, Moorhead, MN 56560.
Abstract View Article
Currently, American Crystal Sugar Company (ACSC) evaluates seeding refractive dissolved solids (RDS) by calculating supersaturation (SSAT) at a given RDS, temperature, and purity. This was traditionally completed utilizing Grut’s coefficients of impure solution solubilities to calculate ysat for a given non-sugar to water ratio (ns/w). During the past several campaigns, ACSC realized the need for higher seeding RDS on batch crystallization pans. The necessity to maintain higher seeding RDS was made evident by realization of increased instances of dissolved mill seed at a target SSAT. To better understand the need for higher seeding RDS at ACSC, an evaluation of sucrose solubility on ACSC’s technical solutions was undertaken. ACSC collected monthly samples of saturated spin-off syrups for high green, intergreen, and molasses during the fiscal years 2024 and 2025 beet slicing and extract campaigns. The samples were prepped to obtain different ns/w ratios. The samples were crystallized with excess sucrose at 80⁰C for 24 hours for each run. Mother liquor was separated from the crystals for each sample pneumatically and evaluated for RDS and purity. This data was used for calculation of qsat,i. The ns/w ratios were charted against the saturation coefficient to obtain a new 4th order polynomial equation. To date, it appears this will be a more accurate representation of ACSC’s technical solutions at a given ns/w for estimating the saturation coefficients. The saturation coefficient of a specific ns/w ratio at a given RDS, purity, and temperature is then used for calculating sucrose supersaturation. ACSC was interested in how much ACSC’s technical solutions sucrose solubility changed over the course of a campaign as non-sugar load shifted through extended storage days.
Refractory lining – A key component for lime kilns.
HUENERLAGE, MICHAEL1*, HARALD MERKER1 and JEREMY TERRY2, 1Refratechnik GmbH, Rudolf-Winkel Strasse 1, 37079 Göttingen, Germany, 2Refratechnik North America Inc., 530 Maryville Centre Drive, Suite 420, St. Louis, MO 63141, U.S.A.
Abstract View Article
A continuously changing demand challenges the lime kiln operation in beet sugar factories. There is the request for increased lime capacities, for a flexibility in fuel usages such as coke vs anthracite or natural gas vs biogas, for an increased lifetime and less maintenance efforts and finally for energy saving measures respectively fuel cost reduction. In some factories the lime kiln is a very long existing asset. The outer steel structure is given, but the inner shape can be adapted by a modified refractory lining to improve the material flow and the air-energy distribution. At the end of the day a chance to improve kiln performance. In detail the paper touches the topics (1) concept of a refractory lining, (2) different requirements for coke or natural gas operation, (3) documentation and reporting as basis for improvements, (4) wear phenomena, (5) chemical infiltration and thermal impacts, (6) damages and port-mortem analysis, (7) veneering and maintenance repairing, (8) energy saving with refractory bricks, (9) warehousing and hydration prevention.
A refractory lining is by far not a consumable. Reliability during the campaign, durability and certain capability to cover process or mechanical events inside the lime kiln makes it necessary to consider a refractory lining as a key component of a lime kiln in a beet sugar factory. The paper will contain drawings, pictures, microscope images and videos and will bring the topic of refractory and the real life inside a lime kiln to the audience.
Integration of SugarsTM Process Simulation Data into dataPARC visualization and analytics.
KAHRE, SCOTT*1, SIGRID BAETH2 and SCOTT HYER2, 1Capstone Technology, 1700 Main St., Suite 222, Washougal, WA 98671 and Sugars International LLC, 305 Inverness Way S, Unit 204, Englewood, CO 80112, 2Amalgamated Sugar Company , 138 W Karcher Road, Nampa, ID 83687.
Abstract View Article
The Amalgamated Sugar Company, in partnership with dataPARC and Sugars International LLC have created a proof-of-concept integration between the SugarsTM mass and energy balance tool and dataPARC factory data visualization and analytics. The new application utilizes factory data from dataPARC to set the SugarsTM model input parameters, then imports the SugarsTM balance results back to dataPARC. These simulation results can be viewed alongside actual factory process data in a single dataPARC trend display, providing insight into potential optimization opportunities, equipment malfunctions, and/or measurement errors.
Efficient operations management at sugar factories with a focus on industrial separators.
GAUDET, CY*1, IRMA ARRIETA1, and STEVE PEACOCK1, 1Amalgamated Research LLC, 2531 Orchard Drive East, Twin Falls, ID 83301.
Abstract View Article
Amalgamated Research LLC (ARi) has been a provider of molasses desugarization system (MDS) technology for the American beet sugar industry. After the equipment is designed, constructed and operating parameters are initially optimized, process stability becomes paramount to ensure optimal performance is maintained. This presentation will provide an understanding of the factors that influence efficient operations management from a holistic standpoint, providing guidance on how to recognize process red flags and the main mechanical aspects that have an effect on process consistency. A discussion on the foundations of the process management hierarchy is provided, and as well as the various maintenance strategies that need to be adopted to eliminate functional failures through early detection, identifying issues before production losses take place. Additionally, base cases on recent MDS commissioning and optimization are discussed, including an overview on how to take advantage of monitoring programs, analytical tools, instrument trends and other elements available for process troubleshooting is included in an attempt to provide managers, supervisors, planners and operators the knowledge that facilitates better decision-making that leads to successful operations.
Non-sugar driven, automated milk of lime flow control.
DEBRULER, CAMDEN R.*1, SCOTT W. HYER1, SIGRID BAETH1, ADAM M. MCMAHON1, 1Amalgamated Sugar Company, 1951 S. Saturn Way, Suite 100 Boise, Idaho 83709.
Abstract View Article
Amalgamated Sugar’s Nampa Idaho facility has historically only had manual control of milk of lime (MOL) flow to the purification process. This prevented the factory from efficiently responding to changes in juice flow or quality, and also led to widespread overliming as there was no real-time visibility on usage. Beginning in crop year 2023, the Nampa facility commissioned new control logic to their MOL dosing system which calculates MOL flow based on mass flow of non-sugars coming in with the raw juice. Non-sugar loading is determined by lab-calculated raw juice AP every hour and fed into the automation. MOL mass flow is calculated with online density measurements on MOL and on sweetwater. The MOL flow splits to pre, cold, and hot limers are user controlled based on lab calculated invert destruction, and optimum prelimer alkalinity. With this new control, Nampa reduced their CaO/NS loading by 20%. This effectively eliminated previous factory bottlenecks due to limekiln throughput limitations, while simultaneously improving limekiln health and stability.
Advancing industrial processes with innovative chromatography solutions.
CHARBONNEAU, ANTOINE*1, ALEXANDRA GIMBERNAT2 and CYRIL LEENHARDT1,1Process Solutions Americas LLC, dba “”APPLEXION””, 560 Peoples Plaza, Suite 281 – Newark, DE 19702, USA, 2APPLEXION SAS, 5 chemin du Pilon, 01700 Saint Maurice de Beynost, France.
Abstract View Article
Chromatography has long been a critical technology across various industries for the separation and purification of complex mixtures. Recent breakthroughs in chromatography technology offer new opportunities for increased efficiency and cost savings for both new and existing industrial sites. This presentation will explore key innovations coming from the numerical world. Advanced digital tools, including AI and digital twin technology, are revolutionizing chromatography systems. These tools provide real-time data monitoring, proactive recommendations, and predictive modeling, enabling operators to optimize system performance, achieve desired outcomes, and reduce resource consumption. An industrial case study will be presented in detail to show the outcomes of the implementation of such technologies. Benefits will be measured not only in terms of operational cost savings but also from an environmental perspective. This presentation will discuss the broad applications and advantages of these innovative chromatography solutions, demonstrating their potential to enhance process efficiency, lower costs, and improve overall operational performance across various industries.
Decarbonizing sugar production through advancements in heat exchange technology.
CASKEY, K. JILL*, 4720 106 Ave SE #250, Calgary, AB T2C 3G5.
Abstract View Article
Advances in cooling technology are providing sugar beet factories with new options when it comes to the dual objective of upgrading outdated equipment and decarbonizing their existing operations. This presentation will explore the approaches that different heat exchange technologies are taking to improve producers’ operational sustainability by reducing energy consumption, greenhouse gas emissions and overall carbon footprint. Specifically, the discussion will focus on vertical plate-style moving bed heat exchangers (MBHEs) that are located just prior to storage or bagging. By cooling sugar indirectly via water in a closed-loop system and avoiding the use of high-horsepower fans, this near-zero emissions solution only consumes an average of 0.4 kWh/ton of sugar – a 90% reduction when compared with typical rotary drums or fluid bed coolers that require upward of ~5 kWh/ton. Other technologies to be discussed will include heat pipe heat exchangers (HPHEs), which, while decades old, has recently been providing industries such as sugar with new opportunities for waste heat recovery. HPHEs have demonstrated their economic value across a range of industries such as food processing, energy and automotive. These systems are well suited at recovering thermal energy from challenging sources such as hot and particle-laden exhaust streams. The presentation will conclude with real-world examples of how these technologies are actively decarbonizing operations worldwide.
A study for the chemical markers of Pseudomonas punonensis.
BUSCHETTE, LYNN,* ELLA MULDER, CONNOR LIUDAHL, STEPHANIE HEIDEN, and DAVID GROOM. American Crystal Sugar Company, 1700 North 11th Street, Moorhead, MN 56560.
Abstract View Article
At American Crystal Sugar (ACS), many microbial species have been identified using deep sequencing of 16S rRNA gene PCR amplicons with the MinION sequencing device (Oxford Nanopore Technologies). These identifications are from the evaluation of sugar beets, sugar beet storage conditions, and factory process samples. There have been many common microbes identified, such as Leuconostoc, Pseudomonas, and Aerococcus. There have also been lesser known microbes found in abundance, such as Gloeothece, Sphingobacterium, and Agronema. Leuconostoc and many of the lactic acid bacteria have been well documented for the metabolites that they generate in the metabolism of sucrose, glucose, and fructose. The lesser known microbes identified at ACS have unknown effects to their existence in the beets and process juices. In a current study, a common species, Pseudomonas punonensis, was evaluated for the potential capability to metabolize sucrose and subsequent metabolite production. P. punonensis, a Gram-negative bacilli, was grown both aerobically and anaerobically at 280 C and below 100 C in tryptic soy broth. The metabolites (chemical markers) monitored at ACS are lactic acid, volatile fatty acids (VFAs), ethanol, and invert sugars (glucose and fructose) by high-performance liquid chromatography (HPLC). This species of Pseudomonas was not able to metabolize sucrose (lack of invertase) as an energy source. It did, however, utilize glucose and fructose as energy sources.
First results in online NIR technology for the beet processing at Pfeifer & Langen.
BURKHARDT, MARK OLIVER*1 and WOLFGANG KLOSTERHALFEN2, 1Pfeifer & Langen IP GmbH Germany, Köln, 2Wolfgang Germany, 50858 Köln, Aachener Strasse 1042 a Pfeifer & Langen GmbH & Co KG.
Abstract View Article
Over the last 5 years, Pfeifer & Langen has been developing an IoT platform to optimize the sugar beet process. In the first step, the focus was on developing and adapting new sensors to control the sugar production processes from beet to silo in real time. One of the adapted technologies is the use of NIR process devices in the sugar process from beet to molasses, which provide real-time feedback on the quality of the raw material, by-products, juices in different production steps and syrups in the final sugar product. All with the intention of supporting operators to run the process proactively. However, the main goal of this development is how the devices support our own developed “predictive models” to run the factory in autopilot mode. The presentation shows our concept of a fully automated process and where we already continuously measure the previously mentioned products. We explain how the data flow works and where and how we can use the data in our general concept of a fully automated process. Finally, we present the products we have developed together with our cooperation partner and how they can also support third parties.
Identification of bacterial isolates from North American sugar beet factories and determination of optimal lab-scale antimicrobial dosing.
BRUNI, GILLIAN O.*1, USDA-Agricultural Research Service, Southern Regional Research Center, 1100 Allen Toussaint Blvd, New Orleans, LA 70124.
Abstract View Article
Microbial-infected sugarbeets with rot along with associated soil microbes are carried into factory processing streams during raw sugar extraction. These microbes consume sucrose and commonly cause downstream processing issues. In particular, bacterial exopolysaccharides often cause operational challenges by clogging filters, increasing viscosity, and interfering with clarification and sucrose crystallization. Therefore, isolation and identification of the microbes and key phenotypes are central toward ongoing efforts to reduce microbial-related sucrose losses. This study was conducted in order to obtain relevant microbial isolates from sugar beet factories to facilitate further investigation into impacts on processing as well as to determine optimal antimicrobial dosing. To this end we conducted a microbial isolation study from juice and biofilm material broadly sampling from eighteen sugarbeet processing factories across North America. Juice and biofilm material was collected by factory operators into cryovials containing glycerol to produce a cryostock that could be frozen while preserving microbial viability until samples could be shipped to the research laboratory in New Orleans, LA and cultured for identification. This effort resulted in 612 isolates. In total, 379 isolates, belonging to 22 genera, were obtained from 33 diffuser tower juice samples, and 233 isolates, belonging to 26 genera, were obtained from 21 biofilm samples. Susceptibility testing of key groups of microbial isolates by microdilution assay has been used to assess the minimum inhibitory concentration (MIC) values of conventional antimicrobial agents to prevent microbial growth. The MIC value of sodium hypochlorite for most Leuconostoc, Weissella, Bacillus, and Peribacillus isolates was typically 250 ppm with a few Leuconostoc outliers showing increased resistance at significantly higher doses. Pantoea and Rahnella typically had higher MIC values near 500 ppm of sodium hypochlorite. MIC doses of Hydritreat 2216 with peracetic acid active ingredient ranged from 63-250 ppm for Leuconostoc, Weissella, Bacillus, Peribacillus, Pantoea, Rahnella, and Acinetobacter isolates. Hops Betastab XL was tested against Gram-positive isolates and showed MIC values typically in the range of 16-31 with a few outlier MIC values for more resistant strains of Leuconostoc above 1000 ppm. Avancid GL50 (glutaraldehyde) showed relatively lower effectiveness against isolates with MIC value ranges of 250-1000+ ppm. Magnacide D showed better inhibition of Gram-negative isolates at MIC values between 16-250 ppm whereas it was generally less effective against Gram-positive isolates. These studies also indicate that high temperature of 50 ℃ or more is advantageous for controlling growth of key microbial contaminants and may be more impactful for processing than some antimicrobial agents.
The ability for realtime measurement of sugarbeet quality on the cossette belt.
BERNHARDSON, DUANE*1, CHAD DAHLEN1 and STANLEY NWADE1, 1KWS Seeds LLC, 1500 S 40th ST, Grand Forks ND 58201.
Abstract View Article
In the manufacturing of most products there is control of the inputs. This control is designed to maintain the standard of the manufacturing process and the quality of the end product. Processing of sugarbeets into sugar involves taking the harvested root, either fresh or stored and converting it into refined sugar. This Primary Processing takes the root and converts it into crystal sugar which allows it to be stored. After entering the plant the sugarbeets are washed and then sliced into cossettes, it is from these cossettes that the sugar is extracted. The cossettes are brought into the process on the ‘cossette belt’ and it is at this stage in the process that samples are collected with a given frequency and analyzed for their quality. The capacity of a sugarbeet plant is measured in tons sliced per hour or day. The slice capacity of a plant can range from 200 tons to over 700 tons per hour. For most plants they take a sample of the incoming cossettes about every 2 hours. This sample provides only a snapshot of the incoming beets due to the large volume of material that can be sliced between the sampling and the range of geography that the beets are received from. To improve the knowledge of the incoming raw good and provide real time information, KWS has developed a methodology to use NIRS technology from its BEETROMETER®. We will share with you how this system is able to provide you with quality values for POL Sugar and Dry Matter and the possibilities that the future holds.
Dimple tube technology – An innovation to improve vacuum pan throughput and factory efficiency.
BEZUIDENHOUDT, DARIUS*1. MEETHAN GOKOOL1 and MURRAY STEVENS1, Bosch Holdings, 23A Flanders Drive, Mount Edgecombe, Durban, 4302, South Africa.
Abstract View Article
An innovative vacuum pan tube has been developed, tested, trialed and commercialized; it provides an alternative solution for increasing the capacity at a significantly lower investment when compared to the market equivalents. This technology has been in operation in numerous vacuum pans, some for several seasons, and the benefits to vacuum pan throughput are significant. The dimple tube technology was developed over a four-year period, from an initial concept through to its commercialization, and it has been through a series of analyses, alterations, tests and trials. These analyses included Computational Fluid Dynamics (CFD) to predict its flow and performance characteristics, Finite Element Analysis (FEA) to analyze the direct and fatigue stresses, pressure testing, non-destructive testing, in-service trials and laboratory testing. Manufacturing equipment and systems have been designed, developed and implemented as part of the commercialization process, in order to ensure the reliable and repeatable production of the technology, which has been taken from a concept, through to a commercially available and value-adding product. The outcome is a new tube technology that increases the heating surface area of an existing vessel by approximately 15%. The performance benefits of the additional heating surface area are as expected, but the increased turbulence makes a significant difference to the heat transfer coefficients, resulting in a significant increase in the evaporation rate and an increase in the throughput. The trial operation has shown no drawbacks or compromises to the existing operations. Retrofitting to the existing vessels is as simple as a retube of the vacuum pan. Due to the improved heat transfer coefficients when utilizing the dimple tube technology, rigorous lab-scale tests and full-scale trials have also shown that the tubes provide the opportunity to utilize lower grade steam to achieve the same heat transfer, enabling energy savings in the factory. Keywords: vacuum pan, tubes, heat transfer, turbulence, continuous vacuum pan, batch vacuum pan, dimple tubes, energy savings
Process development of beet sugar factories: Masterplan for the reduction of fossil energies based on technology improvements.
BAGHERZADEH, ARASH and QUIDO SMEJKAL*, EnProCo Berlin GmbH Volmerstr. 7A 124 89 Berlin, Germany.
Abstract View Article
The rising prices of fossil fuels and increasing costs for environmental protection are prompting sugar manufacturers to invest in innovative processes aimed at reducing production costs. As a result, development projects around the globe are primarily focused on minimizing steam and electricity consumption, as well as optimizing the secondary utilization of waste energy and CO2 emissions. One significant challenge in implementing new technologies at certain sugar factories is the reliance on outdated or under-invested machinery. Depending on the specific technology and processing methods employed in each factory, various scenarios and developmental pathways can be recommended. A major obstacle, particularly for factories with moderate to high energy demands, is the gradual and costly transition required to reach a processing level where these innovative measures can be effectively applied. This paper summarizes insights from a long-term development project involving beet factories, highlighting several technological improvements achieved between 2009 and 2022. It provides a detailed account of experiences in optimizing process technology and reducing manufacturing costs in a form of “master plan” of process development. The contribution presents practical process scenarios of the master plan that have emerged from recent technological advancements. The actual campaign performance is described through footprint balances, leading to identification of possible process modifications. The first scenario focuses on moderate investments aimed at optimizing heat exchange and evaporation. Another scenario discusses the extension of the evaporation station from five to six effects, enhancing optimal vapor utilization. A subsequent scenario examines the impact of low temperature drying on fossil energy demand. Finally, the paper presents a scenario involving vapor compression in both evaporation and sugar house processing. Selected projects are categorized by investment costs, ranging from moderate and mid-term improvements to more strategic investments. The payback period and return on investment are central themes throughout this paper. The primary challenge remains how to reduce the overall energy requirement of the process while simultaneously identifying the right technologies that significantly lower CO2 emissions in a financially viable manner.
Maximizing operational efficiency – A study into proper valve technology selection for critical sugar processing applications.
BAKER, MAX H.*1, 1Bray International, Inc., 13333 Westland East Blvd., Houston, Texas.
Abstract View Article
The North American market has witnessed a significant surge in sugar demand over the past few years. As a result, sugar beet plants are under heightened pressure to escalate their production capabilities while maintaining profitability. Profit margins in the industry are generally low, prompting plant managers to set ambitious efficiency goals to reduce operational disruptions and unplanned outages. One of the crucial aspects of maintaining high efficiency in sugar beet production is through the use of reliable technology. Leading producers are now investing in advanced machinery and equipment designed to last the entirety of their estimated lifespan, as operational hiccups and unplanned outages can have a detrimental effect on production. These issues can be mitigated through the use of advanced technologies and robust equipment that are specifically designed to withstand the demanding conditions of sugar beet processing. In this technical discussion, I will review the major challenges that valves face in critical sugar processing applications. Furthermore, I will drill into the revolutionary valve technologies that mitigate the common problems that stem from the negative effects of the harsh media, while enhancing service life and reliability.
Processing - Poster
Centrifuge control ensures operational safety: state of the art.
SPANGENBERG, DIRK, ANDREAS LEHNBERGER*, EDUARDO LIMA and AREND WITTENBERG, BMA Braunschweigische Maschinenbauanstalt GmbH, Am Alten Bahnhof 5, 38122 Braunschweig, Germany.
Abstract View Article
Occupational and operational safety is in the industry a key issue worldwide, including in sugar refineries. Management is responsible for the safety of employees. While occupational safety is only regulated locally, the manufacturers of equipment are also required to implement operational safety. Batch centrifugals in sugar factories turn large masses at high rotation. The energy stored in the operating phases of acceleration, spinning and deceleration has the potential to end catastrophically in the event of an accident. The manufacturers of batch centrifugals have always been aware of the dangers associated with this energy and have developed strategies to rule out any risk to life and limb. The hazard analysis, which is part of a machine safety assessment, includes the assessment of risks according to the criteria of severity of injury, frequency and duration of exposure and the possibility of hazard prevention. Based on the results, the equipment is designed according to the principles of the assigned safety category. This paper describes the current status of measures on batch centrifugals that are suitable for preventing uncontrollable and potentially dangerous operating and machine conditions. Taking into account the interaction of the rotating centrifugal basket with the drive package and the control unit, the use of fail-safe components and the implementation of safety-enhancing operating sequences are presented. During operation, regular monitoring is necessary to ensure operational safety throughout the entire service life.
Energy savings through fine filtration of high brix standard liquor.
SCHÖPF, STEFAN*, Lenzing Filtration – Division of Lenzing AG, Werkstrasse 2, 4860 Lenzing, Austria.
Abstract View Article
In times of ongoing energy crisis and uncertainties, it is more important than ever before to reduce energy demand wherever possible, same is valid for the sugar industry. This triggered several European beet sugar factories to rethink dilution factors like for example in the remelt of c-massecuite centrifuge run-off, to save on water input as well as evaporation energy. A bottleneck lies within the filtration of these standard liquor streams, as the sugar content is a limiting factor for many available filter systems. Also sugar contents up to 80 brix and even higher are beyond their capability. This is not the case for the Lenzing OptiFil, which already before has proven to be a highly efficient equipment in 100+ sugar installations and was now challenged with the task of filtering standard liquor streams at 80 brix and finest filter ratings between 20 and 50 µm. Conventional automatic filters have not been able to backwash their filter material properly even at coarser filtration ratings (80 µm and coarser), leading to downtime for manual cleaning and requiring already limited manpower for the same. On the other hand, disposable filter bags have been used. However, due to the presence of sticky particulate in high concentrations, these filters required even more attention for exchange of the bags and therefore high operational expenses (OPEX) and manual labor. Not to mention the disposal of the same. On the other hand, the OptiFil – with its patented backwash mechanism – is capable of filtration down to 20 µm and operates fully automatic throughout a full campaign creating reject rates between 0.6 and 1.0 % of the respective inlet flow rate. Due to this fact and the extraordinary short ROI through reasonable energy savings, this filter system was implemented in six different factories throughout Europe in only one year, even more to come. This paper provides a comprehensive outline on the filtration systems tested for highly concentrated standard liquor and describes the implemented solution at several individual installations.
Minimizing volume-weighted mean of seed crystal fondant by optimizing operation of Sweco Ball Mill.
McKEWAN, WILLIAM, HUDI KOBRINSKY, NICK MORITZ*, RAY DOBRATZ and DAVID GROOM, American Crystal Sugar Company, 1700 North 11th Street, Moorhead, MN 56560.
Abstract View Article
The purpose of this experiment was to optimize the operation of a Sweco Ball Mill in order to minimize the volume-weighed mean (D[4,3]) and span in seed crystal fondant (SCF). D[4,3] and span of the SCF was determined using a Malvern Mastersizer 3000 Particle Size Analyzer and a Malvern Hydro SM sample dispersion unit. American Crystal Sugar Company (ACSC) utilizes batch vacuum pans to produce sugar. SCF is added to the vacuum pans at seeding. Uniform and reproducible SCF is essential for ideal operation of batch pans. Initially, a screening design of experiments (DOE) was used to determine the most significant factors. A response surface methodology (RSM) experiment was then used to determine the best setting for each significant factor that minimized D[4,3] and span. The screening DOE and RSM experiments were designed and analyzed using Minitab Statistical Software. With the optimized Sweco Ball Mill producing quality SCF, all five factories at ACSC will have improved batch vacuum pan operations.
Importance of gassing slot sizing on carbonation gas absorption: A computational approach.
ROTAROV, DAMIAN N.*, SEAN R. RICHARDS and MADELINE M. LANGLEY, Michigan Sugar Company, 2600 S. Euclid Ave. Bay City, MI 48706.
Abstract View Article
In 2024, Michigan Sugar Company Bay City identified hampered gas absorption in pre-carbonation and 1st carbonation. Further investigation revealed that the gassing slot sizes were oversized. The oversized slots were theorized to create inconsistent gas flow from each slot. Both standard and computational fluid dynamics analyses were performed to determine the best slot sizing. The first pass analysis using standard fluid dynamics approach determined a test size, and this size was verified using computational fluid dynamics. The slots were adjusted based on the computational analysis, and absorption was remeasured. After modification, absorption in pre-carbonation and 1st carbonation met specification.
Recovery of acetoin, a chemical pre-curser and produced via fermentation of sugar beet extract, using solvents.
KLASSON, THOMAS K.* and EVAN TERRELL, USDA-ARS Southern Regional Research Center, 1100 Allen Toussaint Blvd., New Orleans, LA 70124.
Abstract View Article
Acetoin (3-hydroxybutanone) is a four-carbon ketone-alcohol used in the food industry and is also a precursor to important industrial chemicals such as butanediols and butanols. In this work, we produced acetoin from sugars in sugar beet extract via fermentation using the bacterium Bacillus subtilis. The product was recovered from the fermentation broth with acetone, another product that can be produced via fermentation of beet extract. The results show that extraction was feasible and a process was designed and a cost analysis was performed using SuperPro Designer.
Helping sugar companies understand incoming sugarbeet quality.
DAHLEN, CHAD*, DUANE BERNHARDSON and STANLEY NWADE, KWS Seeds LLC, 1500 S 40th ST, Grand Forks, ND 58201.
Abstract View Article
Current methodology for measuring incoming sugarbeet quality is to collect a sample every several hours from the cossette belt. This process though, only provides a snapshot of the situation with the small sample collected vs the quantity of incoming beets being sliced. The KWS BEETROMETER® Cossette Belt Unit provides automated continuous analysis for real-time information with the ability to be adjusted to align with each individual factories process. The system provides real-time values for POL sugar and Dry Matter to assist operators in understanding the quality and consistency of the incoming sugarbeets. The calibration can be connected to the beet quality lab using the same NIRS process.
Biodegradable and sustainable antifoam based on modified cellulose.
GUEZEL, CEM1, MARCUS JANSCHEL1, VESNA ALEKSANDROVIC-BONDZIC1, VALERI WERNER2, LARS HERWAEGEHR2 and IGOR SEDOV*2, 1Fraunhofer-Instituts für Angewandte Polymerforschung IAP, Grindelallee 117, 20146 Hamburg, 2Schill+Seilacher “Struktol” GmbH, Moorfleeter Strasse 28, 22113 Hamburg.
Abstract View Article
The aim of this project was the development of a biobased and biodegradable antifoam. A sugar factory uses a substantial amount of antifoam during beet sugar production. Most commonly those consist of fossil or silicone raw materials. Despite the growing demand for sustainable and biodegradable, plant-based alternatives, their foam-breaking performance remains inadequate compared to commercial products. The joint project “BIO-DEFOAM” aims to develop a sustainable and effective alternative to the fossil-based antifoams. Cellulose is a renewable and sustainable raw material. The possibility to functionalize cellulose is already being utilized in the chemical industry, making it one of the most consumed renewable polymer. A first batch of antifoam was produced by Frauenhofer IAP based on the syntheses given in the literature (Z. Hu et al. ACS Sustainable Chemistry & Engineering 5 (2017), 5018). Antifoaming capacity of the nanocrystalline cellulose functionalised with tannic acid were tested in a wide range of applications. Especially during the sugar test at elevated temperatures (70°C/158°F), the nanocrystalline cellulose antifoam performed very well, even comparable to commercial products from Struktol (Figure 1. Nano I). Further modifications of the cellulose have been synthesized and tested for their antifoaming capabilities. In a first step the nanocrystalline cellulose was replaced by cheaper micro- & macrofibre cellulose. Secondly, the functionalization with different vegetable oil was tested, in order to eliminate critical chemicals and ease upscaling into industry size (Figure 1. Nano II). The antifoaming capabilities of those formulations with different combinations of cellulose and methods of functionalization can be found in Figure 1. The graphic displays the foam generation in mL by fumigation of air into a solution consisting of water, sugar and saponin. It can be seen, that the bio defoamer based on macro cellulose and functionalized by esterification performs as well as the already commercially available product STRUKTOL SB 2433. The antifoaming capacity of selected bio defoamers based on modified cellulose was found to be in the range of already available products based on fossil raw materials. It remains to be seen if an industrial implementation of the bio defoamers can be viable.
Sensors in beet processing.
BURKHARDT, MARK O*. and WOLFGANG KLOSTERHALFEN, Pfeifer & Langen GmbH & Co KG, 50858 Köln, Germany.
Abstract View Article
Over the last 5 years, Pfeifer & Langen has been developing an IoT platform to optimize the sugar beet process. In the first step, the focus was on developing and adapting new sensors to control the sugar production processes in real time from the beet to the silo with the intention of supporting operators to make the process more proactive. The poster gives a general overview of sensor developments in beet processing from field to silo and presents two examples from a closer perspective: an example of online NIR analysis in molasses and an example with a Visual Smart Sensor in crystallization. The poster also explains the functionality and concept of our integrated platform, where all information is combined into a data system that supports management, engineers, operators and algorithms to better operate production according to requirements. However, all elements of P&L’s own development aim to support the so-called “predictive control models” to enable the factory to operate in autopilot mode.
Soil moisture sensor evaluation in Idaho sugarbeets.
HEGE, ARIANNA*1, DAVEY OLSEN1, CRAIG FINDLAY1, LINDA SCHOTT2 and CLARENCE ROBISON3, 1Amalgamated Sugar-SBR Research, 911 W 8th Street, Burley, ID 83318, 2University of Idaho, Twin Falls Research and Extension Center, 315 Falls Avenue, Evergreen Bldg, Twin Falls, ID 83301, 3University of Idaho, Kimberly Research and Extension Center, 3793 N 3600 E, Kimberly, ID 83341.
Abstract View Article
Precision irrigation management can increase sugarbeet grower profits by improving crop quality, reducing water and pump costs, and building resilience in drought years. Soil moisture sensors provide a method for monitoring soil water status and ensuring crops do not experience drought stress while avoiding excess irrigation applications. The numerous options of commercially available soil moisture sensors can be overwhelming to growers, making it necessary to establish guidelines for navigating the various technology, installation procedures, prices, and performances. This study evaluated the performances of five different types of soil moisture sensors installed in two southern Idaho sugarbeet fields with silt loam and loamy sand soils. Based on metrics of accuracy and precision, sensors ranked from best to worst performance were as follows: Acclima True-TDR315, AquaCheck Sub-Surface Probe, Watermark 200SS, PR2 Profile Probe, GroPoint Profile (installed using a pilot rod), and GroPoint Profile (installed using auger and slurry procedure). Parameters of relative cost and ease of use are also discussed. With the results of this study, growers can make better informed decisions and build confidence in the technology they are investing in for improved irrigation management.